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Addgene
Showing: 4181 - 4200 of 69439 results

  1. pRDA_118

    Plasmid
    #133459
    Purpose
    U6 promoter expresses customizable Spyo-guide; EF1a promoter provides puromycin resistance. This vector is a derivative of the lentiGuide vector, with minor modifications to the tracrR
    Depositor
    John Doench
    Article
    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1
    Insert
    control guide
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    133459-plasmid-map-sequence-id-261403

    Enlarge

  2. pRDA_103

    Plasmid
    #133460
    Purpose
    H1 promoter with two Tet operator (TetO) sites expresses customizable Spyo-guide; short EF1a promoter (EFS) expresses SaurCas9, 2A provides TetR, and 2A provides blasticidin resistance.
    Depositor
    John Doench
    Article
    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1
    Insert
    control guide
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    133460-plasmid-map-sequence-id-261409

    Enlarge

  8. PB CAG-sfGFP

    Plasmid
    #133568
    Purpose
    CAG sfGFP piggyBac transposon
    Depositor
    Mario Capecchi
    Insert
    sfGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    133568-plasmid-map-sequence-id-274027

    Enlarge

  9. PB CAG-tdTomato

    Plasmid
    #133569
    Purpose
    CAG tdTomato piggyBac transposon
    Depositor
    Mario Capecchi
    Insert
    tdTomato
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    133569-plasmid-map-sequence-id-274028

    Enlarge

  10. PB CAG-F-3xSV40pA-F-td-sfGFP

    Plasmid
    #133570
    Purpose
    CAG tdGFP FLP/FRT reporter piggyBac transposon (3x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Flp/frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    133570-plasmid-map-sequence-id-274033

    Enlarge

  11. PB CAG-F-bpA-SV40pA-F-td-sfGFP

    Plasmid
    #133571
    Purpose
    CAG tdGFP FLP/FRT reporter piggyBac transposon (bpA 1x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Flp/frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    133571-plasmid-map-sequence-id-274056

    Enlarge

  12. PB CAG-F-bpA-2xSV40pA-F-td-sfGFP

    Plasmid
    #133572
    Purpose
    CAG tdGFP FLP/FRT reporter piggyBac transposon (bpA 2x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Flp/frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    133572-plasmid-map-sequence-id-274057

    Enlarge

  13. PB CAG-F-bpA-3xSV40pA-F-td-sfGFP

    Plasmid
    #133573
    Purpose
    CAG tdGFP FLP/FRT reporter piggyBac transposon (bpA 3x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Flp/frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    133573-plasmid-map-sequence-id-274063

    Enlarge

  14. PB CAG-L-3xSV40pA-L-td-sfGFP

    Plasmid
    #133574
    Purpose
    CAG tdGFP cre/lox reporter piggyBac transposon (3x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    133574-plasmid-map-sequence-id-274064

    Enlarge

  15. PB CAG-L-bpA-SV40pA-L-td-sfGFP

    Plasmid
    #133575
    Purpose
    CAG tdGFP cre/lox reporter piggyBac transposon (bpA 1x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    133575-plasmid-map-sequence-id-274068

    Enlarge

  16. PB CAG-L-bpA-2xSV40pA-L-td-sfGFP

    Plasmid
    #133576
    Purpose
    CAG tdGFP cre/lox reporter piggyBac transposon (bpA 2x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    133576-plasmid-map-sequence-id-274071

    Enlarge

  17. PB CAG-L-bpA-3xSV40pA-L-td-sfGFP

    Plasmid
    #133577
    Purpose
    CAG tdGFP cre/lox reporter piggyBac transposon (bpA 3x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    133577-plasmid-map-sequence-id-274079

    Enlarge

  18. PB CAG-R-bpA-2xSV40pA-R-td-sfGFP

    Plasmid
    #133578
    Purpose
    CAG tdGFP dre/rox reporter piggyBac transposon (bpA 2x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Dre/rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    133578-plasmid-map-sequence-id-274080

    Enlarge

  19. PB CAG-R-bpA-3xSV40pA-R-td-sfGFP

    Plasmid
    #133579
    Purpose
    CAG tdGFP dre/rox reporter piggyBac transposon (bpA 3x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Dre/rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    133579-plasmid-map-sequence-id-274429

    Enlarge

  20. PB CAG-F-bpA-F-L-td-sfGFP

    Plasmid
    #133580
    Purpose
    CAG tdGFP FLP/FRT reporter piggyBac transposon (bpA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Flp/frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    133580-plasmid-map-sequence-id-274421

    Enlarge

Showing: 4181 - 4200 of 69439 results