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pMSCV-BTK-mCherry

Plasmid

#50043

Purpose

Expression of BTK-mCherry in mammalian cells

Depositor

Hidde Ploegh

Article

Strijbis et al PLoS Pathog. 2013 Jun;9(6):e1003446. doi

Insert

BTK (Btk Mouse)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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Red Glifon 300

Plasmid

#163115

Purpose

Red fluorescent protein-based glucose sensor for mammalian cells with higer affinity

Depositor

Takashi Tsuboi

Article

Mita et al Cell Chem Biol. 2021 Jun 29. pii: S2451-

Insert

Red Glifon 300

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 9, 2021

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_PML_RARA

Plasmid

#205897

Purpose

Express mEGFP-tagged fusion protein, PML_RARA from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

PML_RARA (PML, RARA Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-Fos

Plasmid

#131593

Purpose

doxycycline-inducible expression of mouse c-Fos in mammalian cells

Depositor

Filipe Pereira, Kateri Moore

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

FBJ osteosarcoma oncogene (Fos Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE-mCMV

Available since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0037

Plasmid

#181934

Purpose

Dual secreted luciferase reporter (EF1alpha-Gaussia luciferase, CMV-Cypridina luciferase)

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Inserts

Gaussia luciferase
Cypridina luciferase

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and EF1alpha

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pEF1a-Usp9x_CD-mCherry

Plasmid

#170380

Purpose

Vector for mammalian expression of the Usp9x catalytic domain tagged with mCherry, in pEF1a-IRES-Neo backbone.

Depositor

Miguel Ramalho-Santos

Article

Macrae et al Nat Commun. 2021 Mar 25;12(1):1865. doi:

Insert

Ubiquitin specific protease 9x catalytic domain (Usp9x Mouse)

Use

Tags

mCherry-3xFlag

Expression

Mammalian

Mutation

Catalytic domain

Promoter

Available since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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phage UbiC tagRFP-T-DDX6

Plasmid

#119947

Purpose

Lentiviral vector expressing tagRFP-T-DDX6 fusion protein

Depositor

Jeffrey Chao

Article

Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

DDX6 (DDX6 Human)

Use

Lentiviral

Tags

tagRFP-T

Expression

Bacterial and Mammalian

Mutation

Promoter

UbiC

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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PB-CMV-MCS-Puro

Plasmid

#219794

Purpose

Mammalian expression plasmids using piggyBac Transposon System

Depositor

Keiichi Nakayama

Type

Empty backbone

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_ST-SBP-EGFP

Plasmid

#65264

Purpose

synchronize trafficking of ST from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)

Use

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR-ABCB1_STOP

Plasmid

#221424

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Insert

ABCB1 (ABCB1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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ERmoxGFP

Plasmid

#68072

Purpose

mammalian expression of ER localized moxGFP

Depositor

Erik Snapp

Article

Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

Insert

moxGFP

Use

Tags

KDEL ER retention sequence and bovine prolactin s…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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DHFR-myc

Plasmid

#20214

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

DHFR (folA E. coli)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pSL0341 (pTarget)

Plasmid

#200906

Purpose

Encodes an mCherry casette under a minimal CMV promoter. Used as the target plasmid for plasmid-based integration assays, and for plasmid-based transcriptional activation.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

pTarget

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pTubulin-Electra2

Plasmid

#184930

Purpose

Tubulin labeling with blue fluorescent protein Electra2

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

TUBA1B-Electra2

Use

Tags

Electra2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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TFORF2895

Plasmid

#144355

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

MTA1 (MTA1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-SlugCas9-NNG-P2A-puro

Plasmid

#214352

Purpose

Expresses SlugCas9-NNG and puromycin resistance genes

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

SlugCas9-NNG and puromycin resistance genes

Use

Tags

Expression

Mammalian

Mutation

Q782R/S888R/L906R/N984S/E1012K/K1016I

Promoter

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pTet-O-ASCL1-T2A-PuroR

Plasmid

#162345

Purpose

Lentiviral expression of ASCL1 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

ASCL1 (ASCL1 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Mutation

Promoter

Tet-ON

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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Bcr/Abl P210-pLEF

Plasmid

#38158

Depositor

Nora Heisterkamp

Article

Kweon et al J Biol Chem. 2008 Feb 8;283(6):3023-30.

Insert

BCR/ABL P210 (ABL1, BCR Human)

Use

Tags

GST

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 EGFP-3XNLS

Plasmid

#58468

Purpose

Expresses a nuclear-targeted eGFP on a CMV promoter

Depositor

Dyche Mullins

Article

Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

Insert

3X NLS with 3X stop codons

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pEF1a-IRES-Neo

Plasmid

#28019

Depositor

Thomas Zwaka

Article

Dejosez et al Genes Dev. 2010 Jul 15. 24(14):1479-84.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

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