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pAAV-G-Creon shRNA[Control]

Plasmid

#181824

Purpose

This vector simultaneously expresses GFP protein and shRNA in a Cre-dependent manner.

Depositor

Eun Mi Hwang

Article

Kim et al J Neurosci Methods. 2021 Dec 21:109452.

Type

Empty backbone

Use

AAV and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

EF1a/U6

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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plenti.CamKII.(synapto).iATPSnFR2.A95A.A119L.HaloTag

Plasmid

#209729

Purpose

Expresses presynaptic low affinity ratiometric ATP sensor

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

(synapto).iATPSnFR2.A95A.A119L.HaloTag

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CamKII

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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ER-GA

Plasmid

#209871

Purpose

Dimerization dependent fluorescent protein GA anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1

Depositor

Sarah Cohen

Article

Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

Insert

ddGFP A

Use

Tags

Targeting domain of CYP2C1 MDPVVVLGLCLSCLLLLSLWKQ…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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Integrin β4-GFP

Plasmid

#205092

Purpose

Express GFP-tagged human integrin β4 in mammalian cells

Depositor

Bianxiao Cui

Article

Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

Insert

Integrin beta 4 (ITGB4 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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BII-CMV-AfmW3A

Plasmid

#133402

Purpose

Piggybac vector for constitutive expression of Afamin + Wnt3a for production of conditioned media in HEK293T cells

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

Afamin and Wnt3a (Afm, Wnt3a Mouse)

Use

Tags

Expression

Mammalian

Mutation

N/A

Promoter

CMV

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_ManII-SBP-mCherry

Plasmid

#65253

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin-KDEL and truncated Mannosidase II

Use

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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pMN-303

Plasmid

#195455

Purpose

Lentiviral Integratable mCherry Reporter for synZiFTR ZF10

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

8X ZF10 BS - ybTATA - mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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T7-RelA(K310R)

Plasmid

#23250

Depositor

Warner Greene

Article

Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

Insert

NFkB RelA (RELA Human)

Use

Tags

T7 gp10

Expression

Mammalian

Mutation

K310R

Promoter

Available since

March 15, 2010

Availability

Academic Institutions and Nonprofits only
pCI-SEP_NR2A

Plasmid

#23997

Depositor

Robert Malinow

Article

Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

Insert

NR2A (Grin2a Rat)

Use

Tags

SEP

Expression

Mammalian

Mutation

Promoter

Available since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-Ace-mNeon2-Kv2.1PR

Plasmid

#195528

Purpose

Green fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoter; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

Ace-mNeon2-Kv2.1 proximal restriction sequence

Use

AAV

Tags

Expression

Mammalian

Mutation

Ace-mNeon 81S; SY linker

Promoter

CaMKII

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pTK229

Plasmid

#71256

Purpose

luciferase reposter with 229 bp of the HSV TK promoter

Depositor

Peter Cockerill

Article

Hawwari et al J Immunol. 2002 Aug 15;169(4):1876-86.

Insert

Thymidine Kinase promoter

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

HSV Thymidine Kinase

Available since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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mTLR4

Plasmid

#13085

Depositor

Ruslan Medzhitov

Insert

TLR4 (Tlr4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ApoE3

Plasmid

#197147

Purpose

Expresses ApoE3 in Mammalian cell

Depositor

Bradley Hyman

Article

Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

Insert

ApoE3 (APOE Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn1-SIO-stGtACR1-FusionRed

Plasmid

#105678

Purpose

Expresses a soma-targeted GtACR1 in Cre-expressing neurons

Depositor

Ofer Yizhar

Article

Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

Has Service

AAV5

Insert

stGtACR1

Use

AAV

Tags

FusionRed and Kv2.1

Expression

Mammalian

Mutation

Promoter

hSyn1

Available since

Feb. 23, 2018

Availability

Academic Institutions and Nonprofits only

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bArr1-V5

Plasmid

#201492

Purpose

Expression of the beta-arrestin1 containing a C-terminal V5 tag.

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

bArr1-V5 (ARRB1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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CALCRL-DuET

Plasmid

#213194

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

CALCRL (CALCRL Human)

Use

Tags

1D4 and FLAG

Expression

Mammalian

Mutation

Promoter

CMV, T7

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2-Arl13b-mCardinal

Plasmid

#194542

Purpose

Expresses Arl13b with mCardinal in mammalian cells and zebrafish embryos

Depositor

Heidi Hehnly

Article

Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

Insert

Arl13b (Arl13b Mouse)

Use

Tags

mCardinal

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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IKK-2 WT

Plasmid

#11103

Depositor

Anjana Rao

Article

Mercurio et al Science. 1997 Oct 31. 278(5339):860-6.

Insert

IKK-2 (IKBKB Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pmCherry Vinculin

Plasmid

#50527

Depositor

Kenneth Yamada

Insert

Vinculin (VCL Chicken)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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c-myc-PT3EF1a

Plasmid

#92046

Purpose

Expresses c-Myc in mammalian cells

Depositor

Xin Chen

Article

Liu et al Hepatology. 2017 Mar 30. doi: 10.1002/he

Insert

C-MYC (MYC Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

July 15, 2017

Availability

Academic Institutions and Nonprofits only

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