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Plasmid#211364PurposeLentiviral expression vector for an insert of interest linked to a P2A-T2A puromycin sequence. Produces virus very efficiently. Can also be used for regular transfectionsDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only
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pLKO.1-puro-shNT
Plasmid#109012Purposeshort-hairpin knockdown of targeted geneDepositorInsertNon-targeting shRNA
UseLentiviralTagsExpressionMammalianMutationPromoterhU6 promoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV2-SEP-GluA1 (M1)
Plasmid#64942Purposemammalian expression of rat GluA1DepositorInsertGluA1 (Gria1 Rat)
UseTagsSEP (superecliptic pHluorin)ExpressionMammalianMutationPromoterCMVAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_T2A_iRFP670 sgAAVS1
Plasmid#192477PurposeAll-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1, V2, or V3 cells as a controlDepositorInsertSpCas9
UseTagsT2A iRFP670ExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF0929
Plasmid#143081PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertATOH8 (ATOH8 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFPN1-human Dynamin 1aa K44A
Plasmid#22197DepositorInserthuman Dynamin 1aa K44A (DNM1 Human)
UseTagsGFPExpressionBacterial and MammalianMutationK44APromoterAvailable sinceNov. 2, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-GCaMP6s-WPRE-sv40
Plasmid#184414PurposeAAV expression of Fluorescent calcium reporter, GCaMP6s from TRE promoterDepositorInsertGCaMP6s
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCW57-MCS1-P2A-MCS2 (Neo)
Plasmid#89180PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleav…TagsExpressionMammalianMutationPromoterTREAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
MCP-NMS
Plasmid#210705PurposeThis plasmid express EF1a promoter driven MCP-NMS transactivation moduleDepositorInsertMCP fused to transactivation domain from Human transcription factor NRF2, MRTF-A and STAT1
UseLentiviralTagsEGFPExpressionMammalianMutationN55K in MCPPromoterEF1aAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
BE4-Gam
Plasmid#100806PurposeExpresses BE4-Gam in mammalian cellsDepositorInsertBE4-Gam
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMK287 (mAID-Hygro)
Plasmid#72825PurposeC-terminal tagging with mAID using CRISPR/Cas9DepositorInsertmAID-Hygro
UseCRISPRTagsmAIDExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG_smFP Myc
Plasmid#59757Purposeencodes multiple copies of an epitope tag MycDepositorInsertsmFP _Myc
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceSept. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEnt L1L3 EF1a-tTA-2
Plasmid#26803DepositorInsertElongation Factor 1 alpha promoter and tTA transgene
UseGateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
Equalizer-L episome
Plasmid#169738PurposeEqualizer-L on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.DepositorInsertmiR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterpCMV-tetO2Available sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-NLS-dPspCas13b-3xmRuby3-NLS
Plasmid#132405Purposeoverexpression in human cellsDepositorInsertdPspCas13b
UseLentiviralTagsExpressionMammalianMutationH133A; H1059APromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX208_CMV::mCherry-myc-VAMP2
Plasmid#199716PurposeExpresses VAMP2, fused to mCherry, in mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.DepositorInsertmCherry-myc-VAMP2
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SFXN1_STOP
Plasmid#161477PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSFXN1 (SFXN1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
Flag-HA-COPS6
Plasmid#22542DepositorInsertCOPS6 (COPS6 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceJune 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
Flag-TRIM28
Plasmid#124960PurposeMammalian expression of Human TRIM28DepositorInserthTRIM28 (TRIM28 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only