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Plasmid#64276PurposeExpresses tobacco etch virus protease (TEV)DepositorInsertTobacco etch virus (TEV) protease
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDV05.0_pEF1a-iRFP720
Plasmid#200407PurposeExpresses iRFP720 constitutively in mammalian cellsDepositorInsertiRFP720
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-EGFP
Plasmid#27082PurposeIntegration-free (episomal) expression of EGFPDepositorInserteGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
pICE-HA-MRE11-WT
Plasmid#82033PurposePlasmid for constitutive or doxycycline-inducible expression of wild-type human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorInsertMRE11 (MRE11 Human)
UseTagsHAExpressionMammalianMutationPromoterCMV-tetAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pN7CAST
Plasmid#190661PurposeExpresses polycistronic N7 CAST where Cas12k-T2A-TnsC and TniQ-T2A-TnsB are separated by an EMCV IRESDepositorInserthuman codon optimized N7Cas12k, N7TnsC, N7TniQ, N7TnsB
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST53-LRRK2-G2019S
Plasmid#25045DepositorInsertLRRK2 (LRRK2 Human)
UseTagsGFPExpressionMammalianMutationGlycine 2019 mutated to SerinePromoterAvailable sinceJune 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pZLCv2-3xFLAG-dCas9-HA-2xNLS
Plasmid#106357PurposeEmpty backbone for CRISPR/Cas9 engineered chromatin immunoprecipitationDepositorTypeEmpty backboneUseCRISPR and LentiviralTags3XFLAG and HAExpressionMammalianMutationPromoterU6Available sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-PM-TurboID-V5_IDG-K
Plasmid#214455PurposeGateway destination clone of PM-TurboID (plasma membrane control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertPM-TurboID-V5
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_NUP98_HOXD13
Plasmid#205871PurposeExpress mEGFP-tagged fusion protein, NUP98_HOXD13 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-EGFP-GRAM-W
Plasmid#211704PurposeLentiviral expression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187W mutation (pLJM1-EGFP-GRAM1b G187W)DepositorInsertEGFP-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
UseLentiviralTagsEGFPExpressionMammalianMutationChanged Glycine 187 to TryptophanPromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSH231-EF1-GFP-HYGRO
Plasmid#115144PurposeSafe harbor site 231 knock-in vector with GFP-HygromycinR expression cassetteDepositorInsertGFP
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-miniGFP1-P2A-FusionRed
Plasmid#188732PurposeExpress miniGFP1 and FusionRed in mammalian cellsDepositorInsertminiGFP1
UseAAVTagsFusionRedExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0174
Plasmid#141541PurposeLentiviral vector for overexpressing the RELA transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertRELA (RELA Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C2-NLRP3
Plasmid#73955PurposeExpress EGFP-tagged human NLRP3 in mammalian cellsDepositorInsertNLR family, pyrin domain containing 3 (NLRP3) (NLRP3 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMV iEAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Cre-IRES2-GFP
Plasmid#26646DepositorInsertCre
UseTagsGFP and IRES2ExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag4-OMA1
Plasmid#61719Purposemammalian expression of human Oma1 with C-terminal FLAG tagDepositorInsertOMA1 (OMA1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPBC-G6s
Plasmid#62812PurposepPBC-G6s expresses PiggyBac inverted terminal repeat-flanked GCaMP6s under the control of the CAG promoter.DepositorInsertITR-CAG-GCaMP6s-ITR
UseTagsExpressionBacterial and MammalianMutationPromoterCAGAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
mRNA-CleanCap-NanoLuc
Plasmid#188247PurposeTemplate plasmid to transcribe mRNA encoding NanoLucDepositorInsertNanoLuc
UseLuciferaseTagsExpressionMammalianMutationPromoterT7Available sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
GPR_control
Plasmid#216222PurposeGFP P2A RFP control reporterDepositorInsertGFP P2A RFP_stop
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT126 lenti pEF-rTetR(SE-G72P)-3XFLAG-LibCloneSite-T2A-mCherry-BSD-WPRE
Plasmid#161926PurposeLentiviral backbone for GoldenGate cloning effectors as a fusion with rTetR(SE-G72P)-3XFLAGDepositorInsertrTetR(SE-G72P)
UseLentiviral and Synthetic BiologyTags3XFLAGExpressionMammalianMutationPromoterpEFAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only