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Plasmid#98607PurposeMammalian DamID lentiviral vector for ELK4 with Dam-V5 using Gateway cloningDepositorInsertELK4 (Elk4 Mouse)
UseLentiviral; DamidTagsV5 and Dam (DNA adenine methyltransferase)ExpressionMammalianMutationPromoterHeat Shock Minimal PromoterAvailable sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCitrine-Mito-7
Plasmid#56563PurposeLocalization: Mitochondria, Excitation: 516, Emission: 529DepositorInsertMito (COX8A Human)
UseTagsmCitrineExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSCD-Casp3-L-tev-S
Plasmid#226230PurposeExpress TEVP-cleavable caspase-3DepositorInsertCaspase-3 (CASP3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
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MARS
Plasmid#205232PurposeExpresses PLEKHA5 aa 143-271 (K163A and R164A) fused to mScarlet-i in mammalian cellsDepositorInsertPLEKHA5 aa 143-271 with K163A and R164A mutations (PLEKHA5 Human)
UseTagsNuclear Export Sequence and mScarlet-iExpressionMammalianMutationamino acids 143-271 of PLEKHA5 (GenBank reference…PromoterCMVAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Zeo-ultraID
Plasmid#207704PurposeEmpty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-ultraID cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-CAG-CreiLOV-P2A-FusionRed
Plasmid#192818PurposeExpresses CreiLOV with FusionRed tag in mammalian cellsDepositorInsertCreiLOV
UseAAVTagsFusionRedExpressionMammalianMutationPromoterchicken β-actin promoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF3099
Plasmid#144575PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertKAT7 (KAT7 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-N1-HsSmg5_H
Plasmid#146474PurposeMammalian Expression of HsSmg5DepositorInsertHsSmg5 (SMG5 Human)
UseTagsExpressionMammalianMutationthree non silent mutation A254G (K85R), T980C (L3…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-SuperNovaGreen-T2A-dTomato
Plasmid#193798PurposeExpresses SuperNova Green in the cytosol of mammalian cells, joined by a T2A cleavage site with dTomatoDepositorInsertSuperNova Green
UseAAVTagsFlag tagExpressionMammalianMutationPromoterCAGAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLgw EcoDam-V5-ELK1
Plasmid#98604PurposeMammalian DamID lentiviral vector for ELK1 with Dam-V5 using Gateway cloningDepositorInsertELK1 (Elk1 Mouse)
UseLentiviral; DamidTagsDam (DNA adenine methyltransferase) and V5ExpressionMammalianMutationPromoterHeat Shock Minimal PromoterAvailable sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-DN-hCUL3-FLAG
Plasmid#15820DepositorInsertCullin3 (CUL3 Human)
UseTagsFlagExpressionMammalianMutationDominant negative: N418PromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K33
Plasmid#17607PurposeMammalian expression of HA tagged ubiquitin with K33 only, other lysines mutated to argininesDepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationK33 only, other lysines mutated to arginines; P19…PromoterAvailable sinceMarch 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mNaChBac-T2A-tdTomato
Plasmid#60650PurposeExpresses mNaChBac-T2A-tdTomatoDepositorInsertmNaChBac-T2A-tdTomato
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEJS2601_AAV-Nme2-ABE8e-i1-U6-2xSapI
Plasmid#201683PurposeAll-in-one AAV plasmid expressing Nme2-ABE8e-i1 and U6 sgRNA cassette. SapI enables cloning new spacers.DepositorInsertNme2-ABE8e-i1
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterU1aAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pCMV-Hs.cargo(Cre)
Plasmid#174863PurposeExpresses human Peg10 cargoRNA encoding Cre recombinaseDepositorInsertCre flanked by HsPeg10 UTRs
UseTagsSV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-N1
Plasmid#54767PurposeLocalization: N1 Cloning Vector, Excitation: 488, Emission: 507DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
AAV-hSyn-Ribo-jGCaMP8s
Plasmid#167572PurposePlease see Addgene item #169247 for an improved version of this plasmid. AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s.DepositorInsertRibo-GCaMP8s
UseAAVTags6xHisExpressionMammalianMutationPromoterhSynAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFH2.32_LV-mCherry-MS2x8-BCS
Plasmid#205539PurposeExpress mCherry-MS2x8 with barcodeDepositorInsertmCherry
UseLentiviralTagsExpressionMammalianMutationWTPromoterEF1Available sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only