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Plasmid#194701PurposeExpresses dCas9-KRAB-MeCP2 in AstrocytesDepositorInsertGFAABC1D promoter
UseCRISPR and LentiviralTags3xFLAGExpressionMammalianMutationNonePromoterGFAABC1DAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
AX53_pS.Donor.S1.TS
Plasmid#100290PurposeAAVS1-targeting plasmidDepositorInsert18 bp RFLP (MluI+KspAI)
UseCRISPR and Synthetic Biology; Human targetingTagsExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-RET-V5/HIS
Plasmid#205606PurposeExpression of human RET receptor tyrosine kinase in mammalian cellsDepositorInsertRET receptor tyrosine kinase (RET Human)
UseTagsV5-HisExpressionMammalianMutationPromoterCMVAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLZRS-IRES-deltaNGFR
Plasmid#72930Purposeretroviral expression with FACS-selectable marker (extracellular part of NGFR)DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBA409
Plasmid#85971PurposeEF1a-sfGFP-Ubc-NeoDepositorInsertssfGFP
Neo
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-ACE2-his expression plasmid
Plasmid#158089PurposeExpresses ACE2 in mammalian cellsDepositorInsertACE2 (ACE2 Human)
UseTagsExpressionMammalianMutationSee depositor comments belowPromoterAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
SuExp His-Myc-hPLD3
Plasmid#173851PurposeProtein expression plasmid for recombinant His-Myc tag human PLD3DepositorInsertPLD3 (PLD3 Human)
UseTags6xHis, Myc, Factor X cleavableExpressionMammalianMutationintracellular and transmembrane domain truncated,…PromoterCMVAvailable sinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Sec61β
Plasmid#172445PurposeExpression of ER protein Sec61beta with an N-terminal mCherry tagDepositorInsertProtein transport protein Sec61 subunit beta
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
SPLICS NU-MT P2A
Plasmid#210129PurposeDetect the Nucleus-Mitochondria contactDepositorInsertSPLICS NU-MT P2A
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP1-N1-MAHS
Plasmid#90034PurposeTo express GFP-tagged MAHS in mammalian cells transientlyDepositorInsertMAHS
UseTagsAcGFP1ExpressionMammalianMutationPromoterCMVAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNXF-1_K
Plasmid#146740PurposeMammalian Expression of HsNxf-1DepositorInsertHsNxf-1 (NXF1 Human)
UseTagsExpressionMammalianMutationS144G relative to NM_006362.4PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
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ECFP HO
Plasmid#207413PurposeMammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).DepositorInsertECFP
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
ECFP CD
Plasmid#207414PurposeMammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).DepositorInsertECFP
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF2279
Plasmid#144331PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPRDM2 (PRDM2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHA-N1
Plasmid#86049PurposeA mammalian expression vector with a HA-tag at C-terminus.DepositorTypeEmpty backboneUseTagsHA tagExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ccdB-Renilla
Plasmid#87065PurposeMammalian Gateway-compatible expression vector backbone with a C-terminal Renilla luciferaseDepositorTypeEmpty backboneUseTagsRenilla luciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
EK0211 CMV-TO-BFPmut-stop-t17d9b (FLP-IN)
Plasmid#191139PurposeExpression of Trigger 2 in 3' UTR of an inactive BFP in mammalian cells. Used as second input in OR, AND gatesDepositorInsertt17d9b
UseTagsExpressionMammalianMutationWTPromoterCMV-TOAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
TfR-mCh-DHFR
Plasmid#173013PurposeFor light regulated release of transferrin receptor (TfR) from the endoplasmic reticulum. Driven by CMV promoter.DepositorInsertTfR-mCh-DHFR
UseTagsDHFR and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
EK0294 SFFV-mCherry-SG(s70587-W)SG-EGFP (FLP-IN)
Plasmid#191141PurposePositive control for Sensor 1 (EK0285) where the TAG stop codon is replaced with TGG tryptophan codonDepositorInsertmCherry:s70587-W:EGFP
UseTagsExpressionMammalianMutationUAG>UGG (stop>Trp)PromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only