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Showing: 3001 - 3020 of 71643 results

  4. pQCXIP-cathepsinD-YMH

    Plasmid
    #182644
    Purpose
    Mammalian expression vector encoding a protein fusion composed of human cathepsin D fused to to one tyrosine sulfation (TS) motif (Y), myc (M) and His6 (H) epitope
    Depositor
    Martin Spiess
    Article
    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii
    Insert
    cathepsinD
    Use
    Retroviral
    Tags
    His6-tag, TS site from proCCK, and myc-tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    182644-plasmid-map-sequence-id-358628

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  8. pAAV.TBG.PI.eGFP.WPRE.bGH

    Plasmid
    #105535
    Purpose
    AAV expression of EGFP from TBG promoter
    Depositor
    James M. Wilson
    Has Service
    AAV8
    Insert
    EGFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TBG
    Available since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    105535-plasmid-map-sequence-id-202369

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  10. pKK-BiFC-Venus

    Plasmid
    #105804
    Purpose
    Concomitant expression of two proteins in fusion with fragments of Venus protein. Protein interactions studies by bimolecular fluorescence complementation (BiFC), including BiFC-FRET assays.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    VN173 (I152L); VC155
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105804-plasmid-map-sequence-id-203400

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  16. pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0

    Plasmid
    #174081
    Purpose
    Expression of orthogonal Mb tRNA and tRNA synthetase for incorporation of tetrazine3.0 in eukaryotic cells.
    Depositor
    Ryan Mehl
    Article
    Jang et al J Am Chem Soc. 2020 Apr 22;142(16):7245-
    Insert
    NES-Flag-R2-84 M. barkeri tRNA synthetase for tetrazine3.0
    Use
    Tags
    NES-Flag
    Expression
    Mammalian
    Mutation
    L270G, Y271L, N311G, C313A
    Promoter
    CMV
    Available since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    174081-plasmid-map-sequence-id-345715

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  17. pcDNA3.1.Gαs-LgB113

    Plasmid
    #134362
    Purpose
    Nanoluc complementation assay. Expression of Gαs protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 113 and 114 of Gαs. Addition of the HA epitope at N terminus of Gαs.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    Gαs-LgB113 (GNAS Human)
    Use
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134362-plasmid-map-sequence-id-306402

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  20. myc-BioID2-MCS

    Plasmid
    #74223
    Purpose
    To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present downstream of myc tag and BioID2 tag
    Depositor
    Kyle Roux
    Article
    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    Myc and humanized A. aeolicus BioID2 with R40G
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    74223-plasmid-map-sequence-id-334595

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Showing: 3001 - 3020 of 71643 results