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Plasmid#62777PurposeLocalization: Cilium, N1 Cloning Vector, Excitation:505 / 569, Emission:516 / 581DepositorInsertNPHP4
UseTagsmEos4bExpressionMammalianMutationnonePromoterCMVAvailable sinceMarch 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
P3-Lenti-dCas9-Tet1-GFP
Plasmid#190729PurposeLentiviral construct to express spdCas9-Tet1-GFP.DepositorInsertdCas9-tet1
UseCRISPRTagsSV40 NLS and T2A-EGFPExpressionMammalianMutationChanged Pro434 codon from CCG to CCC and Gly468 f…PromoterUbCAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV.E2.ChETA
Plasmid#220310PurposeDirects ChETA optogenetic actuator to fast spiking interneurons in cortex. Allows for single APs to be evoked.DepositorInsertChETA
UseAAVTagsExpressionMammalianMutationPromoterS5E2Available sinceJune 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-TULP3
Plasmid#218741PurposeExpresses C-terminally EGFP-tagged TULP3 in mammalian cellsDepositorInsertTULP3 (TULP3 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF2771
Plasmid#143614PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertKDM5A (KDM5A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCI-TPI_WT-IL6_3UTR-xrRNA-4H
Plasmid#108373PurposeExpresses wild type TPI reporter; contains partial IL6 3' UTR; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)DepositorInsertUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLPCXU6PT7SK
Plasmid#27549DepositorInsertU6PT7SK (RN7SK Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ZFN-AAVS1
Plasmid#89707PurposeMammalian expression plasmid for zinc finger nuclease targeting human AAVS1 locusDepositorInsertZFN-AAVS1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEF6-Acly(S455D)
Plasmid#70768PurposeExpresses phosphomimetic mouse ATP-citrate lyase in mammalian cellsDepositorInsertATP Citrate Lyase (Acly Mouse)
UseTagsmyc-epitopeExpressionMammalianMutationS455DPromoterEF1alphaAvailable sinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
CRY2olig-mCherry
Plasmid#60032PurposeExpresses a fusion of CRY2PHR E490G with mCherryDepositorInsertCRY2PHR E490G (CRY2 Mustard Weed)
UseTagsExpressionMammalianMutationE490GPromoterCMVAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Cas9_Blast
Plasmid#125592PurposeLentiviral expression of spCas9 with blasticidin resistance geneDepositorInsertCas9
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationPromoterEFS promoterAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDmelOR-mRho.V5.mER.hOr47a
Plasmid#126478PurposeHuman codon-optimized D. melanogaster Orco (hOrco) and Or47a (hOr47a). Or47a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cellsDepositorUseTagsH. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…ExpressionMammalianMutationcodon optimization for H. sapiens and codon optim…PromoterCMVAvailable sinceJune 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
Str-Ii_SBP-mCherry-Golgin84
Plasmid#65304Purposesynchronize trafficking of Golgin-84 from the ER (RUSH system)DepositorInsertGolgin-84 (GOLGA5 Human)
UseTagsStreptavidin Binding Protein (SBP) and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV-EGFP-Cre
Plasmid#86805PurposeLentiviral vector that expresses an EGFP-Cre fusion protein with a nuclear localization signal from the CMV promoterDepositorInsertCre recombinase
UseCre/Lox and LentiviralTagsEGFP and nuclear localization signal: PKKKRKVExpressionMammalianMutationPromoterCMVAvailable sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1 ZoAl-ENT N-1F (R1103A)
Plasmid#216548PurposeFor expression of N-flag tagged ZoAl-ENT R2 protein in mammalian cellsDepositorInsertR2 retroelement protein
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-ultraID-Zeo
Plasmid#207737PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a ultraID-2A-Zeo cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pcDNA3-FLAG-SOX4
Plasmid#110360PurposeMammalian expression of FLAG-tagged SOX4DepositorInsertSOX4 (SOX4 Human)
UseTagsFLAGExpressionMammalianMutationlacking 5' UTRPromoterAvailable sinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -