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Plasmid#142594PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMEF2D (MEF2D Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1429
Plasmid#143115PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNR3C2 (NR3C2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
gTBEv5
Plasmid#220621Purposevector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv5) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoterDepositorInsertnSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
UseTagsExpressionMammalianMutationhUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…PromoterhU6, EF1aAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA/Myc TCF4
Plasmid#16512DepositorInsertTCF4 (TCF7L2 Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceMay 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLEX_305
Plasmid#41390PurposeConstitutive lentiviral expression, SV40-puro; PGK-gateway-no tagDepositorTypeEmpty backboneUseLentiviral; Gateway destination vectorTagsExpressionMammalianMutationPromoterPGKAvailable sinceNov. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCW-GFP-CtIP
Plasmid#71109Purposemammalian expession of GFP-CtlPDepositorInsertCtlP
UseLentiviralTagsGFPExpressionMammalianMutationPromoterTRE promoter, Tet ONAvailable sinceDec. 9, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mEos4b-NPHP4-N-18
Plasmid#62777PurposeLocalization: Cilium, N1 Cloning Vector, Excitation:505 / 569, Emission:516 / 581DepositorInsertNPHP4
UseTagsmEos4bExpressionMammalianMutationnonePromoterCMVAvailable sinceMarch 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBS-L1PA1-CH-mneo
Plasmid#51288Purposecontains the fully codon optimized ORF1 and ORF2 of the human L1RPDepositorInsertL1 (L1RE1 Human)
UseTagsmneoI indicator cassette containing an inverted n…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHRdSV40-K560-24xGCN4_v4
Plasmid#72229PurposeA fusion of the Kinesin-1 motor domain (amino acids 1-560) to the v4 SunTag peptide arrayDepositorInsertK560 kinesin
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
P3-Lenti-dCas9-Tet1-GFP
Plasmid#190729PurposeLentiviral construct to express spdCas9-Tet1-GFP.DepositorInsertdCas9-tet1
UseCRISPRTagsSV40 NLS and T2A-EGFPExpressionMammalianMutationChanged Pro434 codon from CCG to CCC and Gly468 f…PromoterUbCAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV.E2.ChETA
Plasmid#220310PurposeDirects ChETA optogenetic actuator to fast spiking interneurons in cortex. Allows for single APs to be evoked.DepositorInsertChETA
UseAAVTagsExpressionMammalianMutationPromoterS5E2Available sinceJune 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-TULP3
Plasmid#218741PurposeExpresses C-terminally EGFP-tagged TULP3 in mammalian cellsDepositorInsertTULP3 (TULP3 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF2771
Plasmid#143614PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertKDM5A (KDM5A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCI-TPI_WT-IL6_3UTR-xrRNA-4H
Plasmid#108373PurposeExpresses wild type TPI reporter; contains partial IL6 3' UTR; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)DepositorInsertUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Ubiquitin
Plasmid#187910PurposeMammalian cell expression of EGFP-tagged UbiquitinDepositorInsertUBB (UBB Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
N-WASP(CRIB)-mScarlet-I-N-WASP(CRIB)
Plasmid#191451PurposeVisualizing endogenous & active Cdc42DepositorInsertN-WASP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBiFC-VN155(I152L)
Plasmid#27097DepositorInsertVN155(I152L)
UseTagsMycExpressionMammalianMutationI152LPromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pH2B-miRFP720-N1
Plasmid#197231PurposeExpresses the fusion protein of H2B-miRFP720 in mammalian cellsDepositorInsertH2B-miRFP720
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag Runx1 FL (P#1790)
Plasmid#14585DepositorInsertRunx1 (Runx1 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCE-hOCT3/4
Plasmid#41813PurposeNon-integrating (episomal) expression of human OCT3/4DepositorInsertPOU5F1 (POU5F1 Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 9, 2013AvailabilityAcademic Institutions and Nonprofits only