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Plasmid#58766PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Raichu-RhoA-CR
Plasmid#40258DepositorInsertRaichu-RhoA-CR (RHOA Synthetic, Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
Watermelon backbone
Plasmid#155258PurposeCan be used to generate a Watermelon libraryDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMOS028: Mitycam calcium sensor (mitochondrial)
Plasmid#163046PurposeMitycam calcium sensor (mitochondrial)DepositorInsertMitycam
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax-SpG-P2A-hMLH1dn
Plasmid#211550PurposePrime editing in mammalian cellsDepositorInsertPEmax-SpG-P2A-hMLH1dn
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Salsa6f
Plasmid#140188PurposeSalsa6f is a fluorescent ratiometric genetically encoded calcium indicator, (tdTomato linked to GCaMP6f by a V5 epitope tag)DepositorInsertSalsa6f
UseTagstdTomato-v5-GCaMP6fExpressionMammalianMutationPromoterCMV Immediate EarlyAvailable sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-BI-hTFR1
Plasmid#218796PurposeRepCap for AAV productionDepositorInsertAAV Rep-Cap BI-hTFR1
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-YFP-rictor
Plasmid#73387PurposeExpresses HA and YFP tagged RictorDepositorInsertRPTOR Independent Companion Of MTOR, Complex 2 (RICTOR Human)
UseTagsHA and YFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP VAMP2
Plasmid#42308DepositorInsertVAMP 2
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
APEX2-csGBP
Plasmid#108874PurposeExpression of APEX2 fused to a conditionally stable nanobody against GFPDepositorInsertAPEX2-csGBP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHS2 (hANGPTL4-V5)
Plasmid#102446PurposeExpresses V5-tagged human ANGPTL4 in mammalian cellsDepositorInsertANGPTL4 (ANGPTL4 Human)
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLX313-Renilla luciferase
Plasmid#118016PurposeConstitutive expression of Renilla luciferaseDepositorInsertRenilla luciferase
UseLentiviralTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
Arp3-pmCherryC1
Plasmid#27682DepositorInsertArp3 (ACTR3 Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMay 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Kinesin11-N-18
Plasmid#55067PurposeLocalization: Motor Protein/Microtubule Filaments, Excitation: 587, Emission: 610DepositorInsertKinesin11 (KIF11 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pC016 - LwCas13a guide expression backbone with U6 promoter
Plasmid#91906PurposeBackbone for cloning LwCas13a guides under U6 promoterDepositorHas ServiceCloning Grade DNAInsertLwCas13a Guide site
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
FUGW
Plasmid#14883Purpose3rd gen lentiviral plasmid with hUbC-driven EGFP; can be used for cDNA expresionDepositorInsertflap-Ub promoter-GFP-WRE
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRVL-5
Plasmid#104583PurposeContains human IgG1 CH1/CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Full Fc-mediated immune effector functionsDepositorInsertHuman IgG1 CH1/CH2/CH3 (IGHG1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-sgRNA-PGK-puromycin
Plasmid#51133Purposeexpresses sgRNA from the U6 promoter.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
TFORF2258
Plasmid#142927PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSOX11 (SOX11 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
Beta-2-adrenergic receptor-mRFP
Plasmid#67145PurposeBeta-2-adrenergic receptor tagged with mRFP on carboxyl terminusDepositorInsertBeta-2-adrenergic receptor-mRFP (ADRB2 Discosoma sp., Human)
UseTagsmRFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only