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Plasmid#143732PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertGTF2I (GTF2I Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG RpH-LAMP1-3xFLAG
Plasmid#163018Purposeexpresses ratiometric sensor of lysosomal lumenal pHDepositorInsertRpH-LAMP1-3xFLAG (Lamp1 Mouse)
UseTags3XFLAGExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo Fluc EGFP
Plasmid#90170PurposeEGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteinsDepositorInsertFLuc EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag FKHR
Plasmid#13507DepositorInsertFKHR (FOXO1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDY0863 PASTEv4 pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT
Plasmid#179106PurposepCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINTDepositorInsertpCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
PcDNA3.1-Flag
Plasmid#210342PurposeEmpty Vector for molecular cloningDepositorInsertFlag tag
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
VinculinTS
Plasmid#26019DepositorInsertVinculin tension sensor
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pC0042-PguCas13b crRNA backbone
Plasmid#103853PurposeFor cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caacDepositorInsertU6-PguCas13b DR-BbsI-BbsI-polyT
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLDPuro-hsnSR100N
Plasmid#35172DepositorInsertSRRM4 (SRRM4 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Drp1K38A
Plasmid#45161DepositorInsertDrp1 (DNM1L Human)
UseTagsHA (AYPYDVPDY)ExpressionMammalianMutationK38APromoterCMVAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TRE3G-EGFP
Plasmid#52343PurposeAAVS1 donor vector for inducible gene expressionDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas9_GFP
Plasmid#44719PurposeCo-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cellsDepositorInsertCas9-2A-GFP
UseCRISPRTags2A-GFPExpressionMammalianMutationPromoterCAGAvailable sinceApril 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSH-CAG-AtAFB2.F74A-IRES-puro
Plasmid#216245PurposeExpress AtAFB2.F74A mutant without tag for AID2DepositorInsertAtAFB2.F74A
UseCRISPR and TALEN; Human safe harbor locus (a avs1…TagsExpressionMammalianMutationF74A mutationPromoterCAGAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
Twinkle-APEX2-V5
Plasmid#129705PurposeAPEX2 fused to the mitochondrial nucleoid protein TwinkleDepositorUseLentiviralTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax-P2A-hP53DD-P2A-hMLH1dn
Plasmid#214102PurposeMammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and P2A-human MLH1dnDepositorInsertPE-Plus
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
phMYT1L-N174
Plasmid#66809Purpose2nd generation lentiviral transfer plasmid. Expresses human MYT1L with G418 resistanceDepositorInsertMYT1L (MYT1L Human)
UseLentiviralTagsExpressionMammalianMutationPromoterHuman EF1aAvailable sinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR
Plasmid#60229PurposeExpresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.DepositorInsertssgRNA
Cre recombinase
UseAAV, CRISPR, Cre/Lox, and Mouse TargetingTagsCre-HAExpressionMammalianMutationPromoterCBh and U6Available sinceOct. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
GFP-TNFalpha
Plasmid#28089DepositorInsertTNFalpha (Tnf Mouse)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCas9-sgAAVS1-1
Plasmid#129726PurposeExpressing SpCas9 and sgAAVS1-1DepositorInsertSpCas9 and sgAAVS1-1
UseCRISPRTagsP2A-PuroExpressionMammalianMutationPromoterhU6Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_c-MYC
Plasmid#26818DepositorInsert5'UTR-c-MYC-3'UTR (MYC Human)
UseTagsExpressionMammalianMutationSequence begins from second ATG; Synthetic UTR se…PromoterAvailable sinceNov. 17, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits