Addgene

Showing: 1421 - 1440 of 4645 results

Results per page:

Sort results by:

pfast NPC1(NTD)

Plasmid

#25773

Depositor

Johann Deisenhofer

Article

Kwon et al Cell. 2009 Jun 26. 137(7):1213-24.

Insert

npc1 (NPC1 Human)

Use

Tags

His

Expression

Insect

Mutation

aa 23-252 only

Promoter

Available since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only
pFBD-Akt1(K14A)

Plasmid

#86562

Purpose

Expresses human full-length Akt1(K14A) mutant

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Use

Tags

10xHis

Expression

Insect

Mutation

K14A mutation in the PH domain

Promoter

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT-Diaphanous-GFP

Plasmid

#24287

Depositor

Ron Vale

Article

Vale et al J Cell Biol. 2009 Sep 7. 186(5):727-38.

Insert

Diaphanous (dia Fly)

Use

Tags

GFP

Expression

Insect

Mutation

Promoter

Available since

May 10, 2010

Availability

Academic Institutions and Nonprofits only
OA-986B

Plasmid

#124999

Purpose

Expresses dCas9-VPR driven by the Ubiquitin-63E promoter

Depositor

Omar Akbari

Article

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

dCas9-VPR

Use

Piggyback

Tags

Expression

Insect

Mutation

Promoter

Ubiquitin

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pOCC92

Plasmid

#118910

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal and C-terminal eGFP-HIS6

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-HBMss-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette

Use

Tags

eGFP-HIS6

Expression

Insect

Mutation

Promoter

polH

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLib6.4-Aalb_726

Plasmid

#176661

Purpose

Expression of sgRNA under Ae. albopictus U6 (AALF029726) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Tags

Expression

Insect

Mutation

Promoter

Available since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDW445

Plasmid

#8843

Depositor

David Waugh

Article

Duffy et al Anal Biochem. 1998 Sep 10. 262(2):122-8.

Type

Empty backbone

Use

Tags

biotin acceptor peptide

Expression

Insect

Mutation

Promoter

Available since

Nov. 18, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
Hook3(1-522)-SNAPf-Psc-StrepII

Plasmid

#111860

Purpose

C-terminal SNAPf tag on Hook3 (amino acids 1-522) for expression in Sf9 cells. C-terminal SNAPf-Psc-StrepII tag.

Depositor

Andrew Carter

Article

Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

Insert

Hook3(1-552)-SNAPf (HOOK3 Human)

Use

Tags

Psc, SNAPf, and Strep-II

Expression

Insect

Mutation

Optimised for expression in Sf9 cells

Promoter

Available since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pACSV-Ptc

Plasmid

#37675

Depositor

Philip Beachy

Article

Yao et al Cell. 2006 Apr 21;125(2):343-57.

Insert

ptc (ptc Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Actin 5C

Available since

Nov. 12, 2012

Availability

Academic Institutions and Nonprofits only
pUb LwaCas13a + LwaCas13a Guide RNA

Plasmid

#176307

Purpose

Expresses LwaCas13a and its associated guide RNA in Drosophila cells

Depositor

Jeremy Wilusz

Article

Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

Insert

LwaCas13a

Use

Tags

HA tag

Expression

Insect

Mutation

E656G, I1060V, D1076G- please see depositor comme…

Promoter

Ubi-p63e

Available since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLib6.4-Aaeg_763

Plasmid

#176658

Purpose

Expression of sgRNA under Ae. Aegypti U6 (AAEL017763) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
AByF

Plasmid

#111084

Purpose

Integrates marker gene onto D. melanogaster Y chromosome

Depositor

Omar Akbari

Article

Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

Inserts

Y homology arm left
gypsy insulator
3xP3 promoter
tdTomato
SV40 3'UTR
CTCF insulator
Y homology arm right

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
HBM-mVEGF-C-HisTag-pENTR

Plasmid

#64066

Purpose

Plasmid encodes mature form of murine VEGF-C preceded by honey bee melittin secretion signal; entry vector to Gateway cloning system.

Depositor

Joanna Bereta

Article

Klaus et al Protein Expr Purif. 2015 Mar 7. pii: S10

Insert

vascular endothelial growth factor C (Vegfc Mouse)

Use

Tags

His-tag

Expression

Insect

Mutation

Promoter

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pJFRC159-1XUAS-DSCP-B3::PEST

Plasmid

#32137

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

B3::PEST

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pACUH-GFP1-10 sec

Plasmid

#172062

Purpose

UAS vector to express GFP1-10 in the ER lumen

Depositor

Daichi Kamiyama

Article

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

GFP1-10 sec

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
1073B(HomeR2) =pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#2(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]

Plasmid

#159677

Purpose

Plasmid provides the HomeR#2 gene-drive element harboring a rescue, gRNA#2, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #2 via HDR.

Depositor

Omar Akbari

Article

Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

Insert

[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#2(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
1095=Rcd-1r-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

Plasmid

#159673

Purpose

Plasmid supports Cas9 expression in both males and females, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

Depositor

Omar Akbari

Article

Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

Insert

Rcd-1r-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

Use

Tags

T2A-GFP

Expression

Insect

Mutation

Promoter

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pUASP dsRed Rab5 DN

Plasmid

#44823

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab5 (Rab5 Fly)

Use

Drosophila

Tags

dsRed

Expression

Insect

Mutation

S43N (dominant negative)

Promoter

Available since

April 18, 2013

Availability

Academic Institutions and Nonprofits only
p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMycPC

Plasmid

#71812

Purpose

Generating transgenic flies for FLP-inducible DamID

Depositor

Bas van Steensel

Article

Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

Insert

min.hsp70P-FRT-STOP#1-FRT-Dam-Myc-PC

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSK24

Plasmid

#72852

Purpose

template for C-terminal Twin-Strep tag, Puromycin-resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

twin Strep tag

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 4, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge