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Showing: 1261 - 1280 of 4645 results

  4. pUASp YFP Rac1 CA

    Plasmid
    #52235
    Purpose
    Drosophila expression of YFP-Rac1 constitutively active mutant
    Depositor
    Matthew Scott
    Insert
    Rac1 (Rac1 Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    Q61L; CA mutation
    Promoter
    GAL4
    Available since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  10. pFastBacDual with LFn + PrgJ

    Plasmid
    #84869
    Purpose
    Baculovirus Expression of LFn-PrgJ fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel protein
    Depositor
    Russell Vance
    Article
    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi
    Inserts
    LFn
    PrgJ
    Use
    Baculovirus
    Tags
    6xHis
    Expression
    Insect
    Mutation
    Promoter
    Polyhedrin
    Available since
    Nov. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    84869-plasmid-map-sequence-id-162908

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  12. pOCC254

    Plasmid
    #118889
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal mCherry, cleavable with TEV
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-mCherry-TEV-NotI-ccdB-AscI-stop-HindIII cassette
    Use
    Tags
    HIS6-MBP, cleavable with 3C protease; mCherry, cl…
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118889-plasmid-map-sequence-id-232286

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  13. pFastBacI/MDR3cysless

    Plasmid
    #74400
    Purpose
    expression of MDR3 in insect cells
    Depositor
    Robert Nakamoto
    Insert
    MDR3 (Abcb1a Mouse)
    Use
    Tags
    10His
    Expression
    Insect
    Mutation
    all Cys to Ala
    Promoter
    Polyhedrin
    Available since
    March 2, 2018
    Availability
    Academic Institutions and Nonprofits only

  15. pJET1.2[eb-Tc’bhsp-EGFP-2A-Cre; ye-3xP3-gTc’v-SV40]

    Plasmid
    #124069
    Purpose
    CRISPR/Cas mediated knock-in via non-homologous end-joining in Tribolium; bhsp drives EGFP expression; Dm-ebony and Dm-yellow for linearization; (Transformation plasmid; Black eye marker)
    Depositor
    Gregor Bucher
    Insert
    eb-Tc’bhsp-EGFP-2A-Cre; ye-3xP3-gTc’v-SV40
    Use
    CRISPR and Cre/Lox
    Tags
    Expression
    Insect
    Mutation
    Promoter
    bhsp68
    Available since
    July 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    124069-plasmid-map-sequence-id-240664

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  16. His-ZZ-TEV-BICDN(1-400)-GFP

    Plasmid
    #111862
    Purpose
    C-terminal GFP tag on BICDN (amino acids 1-400) for baculovirus expression in Sf9 cells. 8xHis-ZZ N-terminal tag, TEV site to cleave 8xHis-ZZ.
    Depositor
    Andrew Carter
    Article
    Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do
    Insert
    BICDN(1-400)-GFP (Bicd2 Mouse)
    Use
    Tags
    8xHis tag, GFP, and ZZ tag
    Expression
    Insect
    Mutation
    Codon optimized for Sf9 expression
    Promoter
    Available since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    111862-plasmid-map-sequence-id-215382

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  17. pGal80,y+

    Plasmid
    #26851
    Depositor
    Christopher Potter
    Insert
    Gal80 (GAL80 Budding Yeast)
    Use
    Tags
    Yellow Gene (Drosophila)
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Dec. 22, 2010
    Availability
    Academic Institutions and Nonprofits only
    26851-plasmid-map-sequence-id-12719

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Showing: 1261 - 1280 of 4645 results