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Plasmid#63171PurposeDrosophila codon-optimized Flp2::mODC-PEST fusion gene driven from Drosophila Hsp70Bb Heat Shock PromoterDepositorInserthsFlp2::PEST
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSPLp10
Plasmid#170462PurposeEarlyBac insect cell expression systemDepositorInsertNew vector
UseTagsExpressionInsectMutationPromoterdHsp70Available sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pE2c
Plasmid#17462Purposemodified pENTR vector for C-terminal 3x HA tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTags3xHAExpressionBacterial, Insect, Mammalia…MutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pTriEx4-Rac1-2G
Plasmid#66110PurposeSecond generation Rac1 FRET biosensorDepositorInsertSecond generation Rac1 FRET biosensor
UseTagsHis-MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTriExRhoA2G
Plasmid#40176DepositorInsertRhoA Biosensor
UseTagsHis-MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pIZ-Flag6His-BmAgo3
Plasmid#50559PurposeExpresses FLAG, His tagged BmAgo3 in silkworm cell line BmN4 or insect cellsDepositorInsertBmAgo3
UseTags6xHIS and FlagExpressionInsectMutationPromoterOpIE2Available sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pEGFP
Plasmid#165830PurposeEGFP green fluorescent protein CDSDepositorInsertEGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUAST tdTomato Profilin WT
Plasmid#53509DepositorInsertprofilin (chic Fly)
UseTagstdtomatoExpressionInsectMutationPromoterGAL4Available sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSR657
Plasmid#65214PurposeBaculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-2 cap gene in Sf9 insect cellsDepositorInsertsmodified AAV-2 rep78
modified AAV-2 cap
UseTagsExpressionInsectMutationPromoterp10 and polyhedrinAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1 hBRM
Plasmid#1956DepositorInsertBRM (SMARCA2 Human)
UseTagsflagExpressionInsectMutationPromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCoPURO
Plasmid#17533DepositorInsertPuromycine Acetyl Transferase
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
DDB1
Plasmid#25246PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertDDB1_1 (DDB1 Human)
UseBaculovirus expressionTagsHisExpressionInsectMutationPromoterpolyhedrinAvailable sinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
RhoC FLARE.sc mCer, mVenus - CA
Plasmid#65424PurposeRhoC CA biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC Q63LDepositorInsertRBD, mCerulean, mVenus, RhoC
UseTagsExpressionBacterial, Insect, and Mamm…MutationT153M in mVenus, Q63L in RhoCPromoterAvailable sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
RhoC FLARE.sc mCer, mVenus - DN
Plasmid#65423PurposeRhoC DN biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC T19NDepositorInsertRBD, mCerulean, mVenus, RhoC
UseTagsExpressionBacterial, Insect, and Mamm…MutationT153M in mVenus, T19N in RhoCPromoterAvailable sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8m-p10
Plasmid#218862PurposeGal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertRSET-jGCaMP8m
UseTags6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…ExpressionInsectMutationA25G F286YPromoterhsp70Available sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSR646
Plasmid#65215PurposeBaculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-6 cap gene in Sf9 insect cellsDepositorInsertsmodified AAV-2 rep78
modified AAV-6 cap
UseTagsExpressionInsectMutationPromoterP10 and polyhedrinAvailable sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
Ac5-STABLE2-RFP-NLS-CycB(1-266)_GFP-E2F1(1-230)_neo
Plasmid#73164Purposemulti-cistronic Vector for expression for Fly_FUCCI probes in insect cellsDepositorUseTagsmRFP1, GFPExpressionInsectMutationCyclin B Fragment aa 1-266 & E2F1 Fragment aa…PromoterAvailable sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT-Aurora B-GFP
Plasmid#24285DepositorInsertAurora B Kinase (aurB Fly)
UseTagsGFPExpressionInsectMutationPromoterAvailable sinceMay 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSR660
Plasmid#65216PurposeBaculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-8 cap gene in Sf9 insect cellsDepositorInsertsmodified AAV-2 rep78
modified AAV-8 cap
UseTagsExpressionInsectMutationPromoterP10 and polyhedrinAvailable sinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only