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Plasmid#44829DepositorInsertRab11 (Rab11 Fly)
UseDrosophilaTagsdsRedExpressionInsectMutationPromoterAvailable sinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
ORCO-T2A-QF2
Plasmid#162524PurposePlasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Orco geneDepositorInsertOrco-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Orco-right-HDR-arm
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMT-HGP-v3-Nb127D01-hIgG
Plasmid#171564PurposeExpresses hIgG-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-human IgG-TEV-Avi-His) in fly cellsDepositorInsertNb127D01-human IgG-TEV-Avi-His (CXCR2 Human)
UseTagsBiP signal peptide and hIgG1-hinge-N297A-TEV site…ExpressionInsectMutationPromoterAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMT-HGP-v3-NbVHH05-hIgG
Plasmid#171565PurposeExpresses hIgG-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-human IgG-TEV-Avi-His) in fly cellsDepositorInsertNbVHH05-human IgG-TEV-Avi-His (UBE2J1 Human)
UseTagsBiP signal peptide and hIgG1-hinge-N297A-TEV site…ExpressionInsectMutationPromoterAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBacM13-8His-ZZ-LTLT-Hook3-SNAPf
Plasmid#130976PurposeExpression of 8His-ZZ-LTLT tagged Hook3-SNAPf in insect cells for protein purification.DepositorInsertHook3-SNAPf (HOOK3 Human)
UseTags8His-ZZ-LTLT and SNAPfExpressionInsectMutationPromoterPolyhedrinAvailable sinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMKInt-hyg
Plasmid#18953DepositorInsertphiC31 integrase (int Streptomyces phage phiC31)
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-PARP1-Flag-His-7xKQ
Plasmid#111573PurposeExpresses full length PARP1 with K to Q mutations in automodification domain in Sf9 (insect) cellsDepositorInsertPARP1 (PARP1 Human)
UseTagsC-terminal 6xHis and C-terminal FlagExpressionInsectMutationlysine to glutamine mutation at the following ami…Promoterpolyhedrin promoterAvailable sinceJuly 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
RBBP4
Plasmid#25350PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertRBBP4-13 (RBBP4 Human)
UseBaculovirus expressionTagsHisExpressionInsectMutationPromoterpolyhedrinAvailable sinceAug. 19, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBD-ESCRT 0
Plasmid#21499DepositorInsertpFastBac Dual-His/MBP/Hrs-GST/STAM1 (STAM Human, Mouse)
UseTagsGST and His-MBPExpressionInsectMutationnonePromoterAvailable sinceSept. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMT-HA-STX1
Plasmid#89310PurposeExpresses HA tagged syntaxin 1 in Drosophila cellsDepositorInsertSTX1 (Syx1A Fly)
UseTagsHA tagExpressionInsectMutationPromoterP-MT (Metallothionein)Available sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDSAY
Plasmid#62291Purposedocking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, YFP markerDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUASp-Egl-Trbo
Plasmid#179993PurposeExpresses Egl fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system.DepositorInsertEgl-Trbo
UseTagsTurboIDExpressionInsectMutationPromoterUAS-Gal4Available sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-gamma-TuSC
Plasmid#178079PurposeInsect cell expression of gamma-TuSCDepositorUseTagsTEV-His6ExpressionInsectMutationPromoterAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
AT1G66830 X005_pECIA14
Plasmid#114810PurposePrey vector AT1G66830 X005_pECIA14 should be used with bait vector AT1G66830 X005_pECIA2.DepositorInsertAT1G66830 (AT1G66830 Mustard Weed)
UseTagsExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOPINF Ta-sro1 WWE-PARP
Plasmid#192550PurposeE. coli expression vector for Triticum aestivum sro1 WWE-PARP domains (amino acids 2-434)DepositorInsertSIMILAR TO RCD ONE 1 (SRO1) WWE-PARP domains, cultivar Shanrong No. 3 (SR3)
UseTagsHis6, 3C protease cleavage siteExpressionBacterial, Insect, and Mamm…MutationPromoterT7 promoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAcGP67A-mMD2-proA
Plasmid#187878Purposebaculovirus protein expression of mouse MD-2 (residues 19–160) fused to a protein A tagDepositorInsertMD2 (Ly96 Mouse)
UseTagsExpressionInsectMutationincludes residues 19–160 of mouse MD-2PromoterAvailable sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
UAS-FRT-GFP-FRT-u(M)Cas9
Plasmid#127388PurposeCas9 inducible by removal of a GFP flip-out cassetteDepositorInsertFRT-GFP-FRT-uORF(M)Cas9
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
p10xUAS-IVS- Syn21-Voltron-p10
Plasmid#119041PurposeUAS-driven expresion of Voltron in DrosophilaDepositorInsertVoltron
UseTagsExpressionInsectMutationPromoterhsp70Available sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJFRC173-20XUAS-IVS-Dre::PEST
Plasmid#32146DepositorInsertDre::PEST
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-let-7 2x
Plasmid#20929DepositorInsert2x let-7 target sites
UseLuciferaseTagsExpressionInsect and MammalianMutationPromoterAvailable sinceJune 19, 2009AvailabilityAcademic Institutions and Nonprofits only