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pOCC305

Plasmid

#118898

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with 3C and C-terminal HIS6, cleavable with 3C

Depositor

PEPC MPI CBG, Marc Bickle

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-TwinStrep-MBP-3C-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette

Use

Tags

HIS6, cleavable with 3C protease and TwinStrep-MB…

Expression

Insect

Mutation

Promoter

polH

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pOEM-GED-EGFP

Plasmid

#102782

Purpose

Baculovirus rescue vector, VSVGED pseudotype, eGFP

Depositor

Elly Tanaka

Article

Oliveira et al Dev Biol. 2018 Jan 15;433(2):262-275. do

Insert

EGFP

Use

Baculoviral rescue shuttle vector

Tags

Expression

Insect and Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac-HS-NLRC4Δcard

Plasmid

#177276

Purpose

Purification of mouse NLRC4 (90-1024) from insect cells

Depositor

Hao Wu

Article

Zhang et al Science. 2015 Oct 23;350(6259):404-9. do

Insert

NLRC4 (Nlrc4 Mouse)

Use

Tags

HIS-SUMO-PreScission

Expression

Insect

Mutation

I586T, N761S, M885V, K915R and F972L- please see …

Promoter

polyhedrin

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pMT-Pav-GFP

Plasmid

#24286

Depositor

Ron Vale

Article

Vale et al J Cell Biol. 2009 Sep 7. 186(5):727-38.

Insert

Pavarotti (pav Fly)

Use

Tags

GFP

Expression

Insect

Mutation

Promoter

Available since

June 29, 2010

Availability

Academic Institutions and Nonprofits only
pXL-BACII-attPGAL4LWL

Plasmid

#26260

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

GAL4 (GAL4 Budding Yeast)

Use

Drosophila transgenesis

Tags

Expression

Bacterial and Insect

Mutation

Promoter

Available since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pFastBac mph1 flag

Plasmid

#1985

Depositor

Robert Kingston

Article

Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

Insert

mPh1 (Phc1 Mouse)

Use

Tags

flag

Expression

Insect

Mutation

Promoter

Available since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
pHW-CIBN-MP-HFW-CRY2-VHH(GFP)

Plasmid

#126027

Purpose

express Flag-tagged LARIAT for optogenetic clustering

Depositor

Eurico Morais-de-Sá

Article

Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

Insert

CIBN-MP; CRY2 PHR-VHH(GFP)

Use

Tags

Flag

Expression

Insect

Mutation

WT

Promoter

Hsp70

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pX4

Plasmid

#183903

Purpose

donor plasmid for CRISPR Cas9 knockin near the Aedes aegypti m / M locus

Depositor

Eric Marois

Article

Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

Insert

GFP

Use

Tags

Expression

Insect

Mutation

Promoter

Aedes aegypti PUb

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pX3

Plasmid

#183904

Purpose

donor plasmid for CRISPR Cas9 knockin near the Aedes aegypti m / M locus

Depositor

Eric Marois

Article

Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

Insert

GFP

Use

Tags

Expression

Insect

Mutation

Promoter

Aedes aegypti Polyubiquitin

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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47 pLIB_GST

Plasmid

#160272

Purpose

N-term 3C cleavable GST tag for insect cell expression

Depositor

John Weir

Article

Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

Type

Empty backbone

Use

Tags

GST

Expression

Insect

Mutation

Promoter

polyhydrin

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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907L

Plasmid

#160237

Purpose

FlightN-ttgR-VP16-Sv40-attB

Depositor

Omar Akbari

Article

Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

Inserts

TtgR repressor
Flightin promoter

Use

Tags

VP16

Expression

Insect

Mutation

Promoter

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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mEos3-caRac1

Plasmid

#92041

Purpose

Expresses mEos3 fusion with caRac1 (Q61L)

Depositor

Ji Yu

Article

Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

Insert

RAC1 (RAC1 Human)

Use

Tags

mEos3

Expression

Bacterial, Insect, and Mamm…

Mutation

Q61L (constitutively active)

Promoter

CMV

Available since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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pKM377

Plasmid

#134210

Purpose

Baculovirus expression of CENP-HKM and His-CENP-I

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

CENP-H/K/M untagged + his-I

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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EKAR2G_design1_mTFP_105_Venus_195

Plasmid

#39821

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

Erk activity reporter

Use

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

CMV

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pF3BGX-T2A-GAL80

Plasmid

#138396

Purpose

RMCE vector for generating C-terminal T2A-GAL80 fusion

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

T2A-GAL80

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pAcGP67B MBP-tagged human Norrin ( 31–133)

Plasmid

#132625

Purpose

expresses MBP-tagged human Norrin ( 31–133) in Hi5 insect cell

Depositor

Wenqing Xu

Article

Shen et al Cell Res. 2015 Sep;25(9):1078-81. doi: 1

Insert

MBP-Norrin ( 31–133)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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EKAR2G_design1_mTFP_wt_Venus_wt

Plasmid

#39813

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

Erk activity reporter

Use

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

CMV

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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hs-calx1

Plasmid

#1740

Depositor

Craig Montell

Article

Wang et al Neuron 2005 Feb 3;45(3):367-78.

Insert

calx1 (Calx Fly)

Use

Tags

Expression

Insect

Mutation

N/A

Promoter

Available since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
hs-calx2

Plasmid

#1741

Depositor

Craig Montell

Article

Wang et al Neuron 2005 Feb 3;45(3):367-78.

Insert

calx2 (Calx Fly)

Use

Tags

Expression

Insect

Mutation

N/A

Promoter

Available since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
pJFRC168-21XUAS-RSRT>-dSTOP-RSRT>-myr::RFP

Plasmid

#32143

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

RSRT> STOP STOP RSRT>

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 2, 2011

Availability

Academic Institutions and Nonprofits only

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