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pBacPAK8-His-myc-Rbx1

Plasmid

#29506

Depositor

Ronald Conaway, Joan Conaway

Article

Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

Insert

Rbx1 (Rbx1 Mouse)

Use

Tags

His and myc

Expression

Insect

Mutation

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pAC5.1-dCry-V5His

Plasmid

#136237

Purpose

Protein expression of D. melanogaster (Dm) cryptochrome with C-terminal V5 and His tags

Depositor

Steven Reppert, Aziz Sancar

Article

Zhu et al Curr Biol. 2005 Dec 6;15(23):R953-4. doi

Insert

DmCry

Use

Tags

His and V5

Expression

Insect

Mutation

Promoter

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pOCC120

Plasmid

#118892

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and C-terminal monomeric GFP and HIS6, cleavable with TEV

Depositor

PEPC MPI CBG, Marc Bickle

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-MBP-3C-NotI-ccdB-AscI-mGFP-3C-HIS6-stop-HindIII cassette

Use

Tags

MBP, cleavable with 3C protease and mGFP, HIS6, c…

Expression

Insect

Mutation

Promoter

polH

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pIHEU_miniSOG2 T2A H3.3A-EGFP

Plasmid

#87411

Purpose

UAS vector to express Histone 3.3A GFP fusion and miniSOG2 in Drosophila

Depositor

Xiaokun Shu

Article

Makhijani et al Cell Chem Biol. 2017 Jan 19;24(1):110-11

Insert

mini singlet oxygen generator 2

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pUASTattB-p65AD::CaM

Plasmid

#64717

Purpose

generating transgenic TRIC flies

Depositor

Liqun Luo

Article

Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

Insert

p65AD::CaM

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

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pOCC65

Plasmid

#118865

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal monomeric GFP

Depositor

PEPC MPI CBG, Marc Bickle

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-HIS6-3C-mGFP-NotI-ccdB-AscI-stop-HindIII cassette

Use

Tags

HIS6, cleavable with 3C protease, mGFP

Expression

Insect

Mutation

Promoter

polH

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pFastBac mPh1

Plasmid

#1970

Depositor

Robert Kingston

Article

Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

Insert

mPh1 (Phc1 Mouse)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
mEos3-dnRac1

Plasmid

#92040

Purpose

Expresses mEos3 fusion with dnRac1 (T17N).

Depositor

Ji Yu

Article

Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

Insert

RAC1 (RAC1 Human)

Use

Tags

mEos3

Expression

Bacterial, Insect, and Mamm…

Mutation

T17N (dominant negative)

Promoter

CMV

Available since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCaSpeR-act5cB-QF#7

Plasmid

#46125

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

QF#7

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pTK

Plasmid

#165841

Purpose

ganciclovir sensitivity marker

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

SR39TK

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pOPINO

Plasmid

#41140

Depositor

Ray Owens

Type

Empty backbone

Use

Tags

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

March 27, 2013

Availability

Academic Institutions and Nonprofits only

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pLib6.6B

Plasmid

#176666

Purpose

Expression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB1-2-PT-SA-SD-2-mTagBFP2(11x7)

Plasmid

#172066

Purpose

Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 2 coding intron

Depositor

Daichi Kamiyama

Article

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

mTagBFP2(11x7)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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RP156 pUAST EGFP:Gr21a

Plasmid

#59524

Purpose

N-terminal fusion of EGFP to Drosophila melanogaster Gr21a

Depositor

Leslie Vosshall

Article

Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

Insert

EGFP:Gr21 (Gr21a Fly)

Use

Tags

EGFP

Expression

Insect

Mutation

Promoter

Available since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only
874V=βTub-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

Plasmid

#159672

Purpose

Plasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

Depositor

Omar Akbari

Article

Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

Insert

βTub-NLS-hSpCas9-T2A-GFP

Use

Tags

T2A-GFP

Expression

Insect

Mutation

Promoter

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pPVxRF3

Plasmid

#138385

Purpose

Venus knock-in cassette

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

Venus

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOV2 C450A

Plasmid

#81031

Purpose

Mammalian expression of the dark state of LOV2

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV2

Use

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

C450A

Promoter

Available since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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ArpC4_pLib

Plasmid

#173681

Purpose

ArpC4 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

Depositor

Roberto Dominguez

Article

Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

Insert

ArpC4 (ARPC4 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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BacPAK Myc-S1

Plasmid

#19424

Depositor

Ronald Conaway, Joan Conaway

Article

Yao et al Nat Cell Biol. 2006 Sep . 8(9):994-1002.

Insert

Rpn2 (PSMD1, RPN2 Human)

Use

Tags

His and Myc

Expression

Insect

Mutation

Promoter

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
13XLexAop2 IVS Jaws mCherry_tr

Plasmid

#111551

Purpose

Optogenetics in Drosophila: LexAop2-induced expressionof Jaws mCherry

Depositor

Vivek Jayaraman

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws_mCherry_trafficked

Use

Tags

Expression

Insect

Mutation

wt

Promoter

hsp70

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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