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Plasmid#109191PurposeEncodes full-length engineered CD64 with C-terminal Twin-Strep tag to be expressed in a baculovirus/insect cell expression systemDepositorInsertFull-length engineered CD64, derived from human CD64
UseTagsTwin-StrepExpressionInsectMutationengineered for efficient expression, see publicat…PromoterPolyhedrinAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rac1 DN
Plasmid#52234PurposeDrosophila expression of YFP-Rac1 dominant negative mutantDepositorInsertRac1 (Rac1 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationT17N; DN mutationPromoterGAL4Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmStaufen_B
Plasmid#145947PurposeInsect Expression of DmStaufenDepositorInsertDmStaufen (stau Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUASTattB_NSnoFbox-vhhGFP4
Plasmid#35578DepositorInsertNSnoFbox-vhhGFP4 (slmb Fly)
UseTagsExpressionInsectMutationF box domain of slmb deleted.PromoterAvailable sinceApril 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pOPINMSYB
Plasmid#41118DepositorTypeEmpty backboneUseTagsExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceMarch 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKL
Plasmid#110741PurposePlasmid for the insect cell expression system, acceptor for plasmid fusions, two promoters, Multiplication Module M, Tn7 transposition and LoxP siteDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterPolyhedrin and p10 promotersAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
1161F
Plasmid#183138PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 3 gRNA targeting D.suzukii bTub,Hr5Ie1-eGFP tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[sxl, bTub]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
5XUAS IVS CsChrimson tdtomato_tr
Plasmid#111545PurposeOptogenetics in Drosophila: UAS-induced expression of Chrimson tdTomatoDepositorInsertCsChrimson_tdtomato_trafficked
UseTagsExpressionInsectMutationwtPromoterhsp70Available sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1 PIK3R1-Ni
Plasmid#17744DepositorInsertPIK3R1-Ni (PIK3R1 Human)
UseTagsExpressionInsectMutationPartial cDNAPromoterAvailable sinceApril 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCoofy27
Plasmid#44003DepositorTypeEmpty backboneUseTagsHis6ExpressionInsectMutationPromoterPPHAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
U6-BbsI-crRNA
Plasmid#51026PurposeGenerates crRNA for use in combination with tracrRNADepositorInsertU6-BbsI-crRNA
UseCRISPRTagsExpressionInsectMutationPromoterU6-2Available sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCaSpeR4-tubulin-QF#7m1
Plasmid#46128DepositorInsertQF#7m1
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTSTEP-SNAPf
Plasmid#72335PurposeTissue-specifically swappable (C-terminal TagRFPT-to-SNAPf) genome tagging plasmid with Golic+ selection cassette, for fully transgenic gene targeting (Drosophila melanogaster)DepositorInsertRRS-TagRFPT-p10-[Golic+] RRS-SNAPf
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFastBacDual with LFn + FlaAAA
Plasmid#84867PurposeBaculovirus Expression of LFn-FlaAAA fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.DepositorInsertsLFn
FlaAAA
UseBaculovirusTags6xHisExpressionInsectMutation3 C-terminal leucines have been replaced by alani…PromoterPolyhedrinAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]Myc[open]
Plasmid#71805PurposeGenerating transgenic flies for 4-hydroxytamoxifen-inducible DamIDDepositorInsertFL.hsp70P-Dam[4-HT-intein@L127C]-Myc[open]
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
1073A(HomeR1)=pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
Plasmid#159676PurposePlasmid provides the HomeR#1 gene drive element harboring a rescue, gRNA#1, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #1 via HDR.DepositorInsertpBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJFRC2-INS
Plasmid#26215DepositorInsertmCD8::GFP
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1/V5-HisA-RvCAHS12-GS-AcGFP1
Plasmid#192471PurposeTransient expression of RvCAHS12 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cellsDepositorInsertCAHS12
UseTagsAcGFP1ExpressionInsectMutationPromoterAc5Available sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRB39
Plasmid#72870Purposetemplate for N-terminal eGFP tag, constitutive Puromycin resistanceDepositorInserteGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFastBac flag Bmi1
Plasmid#1967DepositorInsertBmi1 (Bmi1 Mouse)
UseTagsflagExpressionInsectMutationPromoterAvailable sinceMarch 23, 2006AvailabilityAcademic Institutions and Nonprofits only