Addgene

Showing: 81 - 100 of 4645 results

Results per page:

Sort results by:

pFastBac-Dual-M2

Plasmid

#135584

Purpose

High eukaryotic dual protein expression shuttle vector

Depositor

Xiao-Wen Cheng

Article

Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

Type

Empty backbone

Use

Transfer vector

Tags

Expression

Insect

Mutation

Promoter

polyhedrin of AcMNPV

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pIZ-EGFP-MYO10-HMM

Plasmid

#87257

Purpose

Expression plasmid to perform NanoSPD assays in insect cells (generates EGFP-tagged bait).

Depositor

Thomas Friedman

Article

Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

Insert

MYO10 (Myo10 Mouse)

Use

Tags

Expression

Insect

Mutation

Myo10 is truncated to contain aa.1-941

Promoter

OpIE2

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-HIS-TEV-CKS1

Plasmid

#177013

Purpose

Generate baculovirus for insect cell expression of CKS1 with N-terminal Polyhistidine-TEV

Depositor

Andrea Musacchio

Article

Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

Insert

CKS1 (CKS1B Synthetic, Human)

Use

Tags

Polyhistidine-TEV

Expression

Insect

Mutation

codon-optimised for insect cell expression

Promoter

Available since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCFD5_w

Plasmid

#112645

Purpose

Ubiquitous expression of one or multiple sgRNAs in Drosophila. Selection marker: Mini-white.

Depositor

Michael Boutros

Insert

U6:3-tRNA-sgRNA expression cassette

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pUAST-pHuji-GINKO2

Plasmid

#177117

Purpose

Genetically encoded green fluorescent potassium ion indicator GINKO2 and red fluorescent pH indicator pHuji fusion

Depositor

Robert Campbell

Article

Wu et al PLoS Biol. 2022 Sep 6;20(9):e3001772. do

Insert

pHuji-GINKO2

Use

Tags

Expression

Insect

Mutation

Promoter

hsp70 promoter

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-GST-3C-CDK1

Plasmid

#177011

Purpose

Generate baculovirus for insect cell expression of CDK1 with N-terminal GST-3C (PreScission)

Depositor

Andrea Musacchio

Article

Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

Insert

CDK1 (CDK1 Human)

Use

Tags

GST-3C

Expression

Insect

Mutation

codon-optimised for insect cell expression

Promoter

Available since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-HIS-TEV-scCAK1

Plasmid

#177014

Purpose

Generate baculovirus for insect cell expression of scCAK1 with N-terminal Polyhistidine-TEV

Depositor

Andrea Musacchio

Article

Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

Insert

CAK1 cyclin-dependent protein kinase-activating kinase CAK1 (CAK1 Budding Yeast)

Use

Tags

Polyhistidine-TEV

Expression

Insect

Mutation

Promoter

Available since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-HIS-TEV-Cyclin-B

Plasmid

#177012

Purpose

Generate baculovirus for insect cell expression of Cyclin-B with N-terminal Polyhistidine-TEV

Depositor

Andrea Musacchio

Article

Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

Insert

Cyclin B1 (CCNB1 Human)

Use

Tags

Polyhistidine-TEV

Expression

Insect

Mutation

codon-optimised for insect cell expression

Promoter

Available since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-Rab5

Plasmid

#31733

Depositor

Ron Vale

Article

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Rab5 (Rab5 Fly)

Use

Tags

GFP

Expression

Insect

Mutation

Promoter

Actin

Available since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
pAc-sgRNA-Cas9

Plasmid

#49330

Purpose

Expresses sgRNA and Cas9-Puro in Drosophila S2 cells

Depositor

Ji-Long Liu

Article

Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

Inserts

Cas9
dU6-sgRNA

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Insect

Mutation

Human codon optimised

Promoter

Actin-5c and Drosophila U6

Available since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
MUNC18-2

Plasmid

#186687

Purpose

Expresses MUNC18-2 in insect cells

Depositor

Axel Brunger

Article

Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

Insert

MUNC18-2 (Stxbp2 Rat)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTriEx-mCherry-PA-Rac1-C450A

Plasmid

#22028

Purpose

Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

PA-Rac1-C450A (RAC1 Avena sativa (oat), Human)

Use

Tags

6xHis and mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

LOV(Leu404-Leu546, C450A). Rac1 starts at Ile4 an…

Promoter

CMV, p10

Available since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pACEBac1-PARP1-Flag-His-WT

Plasmid

#111572

Purpose

Expresses full length PARP1 in Sf9 (insect) cells

Depositor

Thomas Muir

Article

Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

Insert

PARP1 (PARP1 Human)

Use

Tags

C-terminal 6xHis and C-terminal Flag

Expression

Insect

Mutation

Promoter

polyhedrin promoter

Available since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiex-1 GFPNanobody_SNAP

Plasmid

#82711

Purpose

Expresses a GFP binding nanobody linked to a SNAP tag in bacteria and Sf9 cells.

Depositor

Sivaraj Sivaramakrishnan

Article

Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

Insert

GFP Nanobody

Use

Tags

6xHis Purification tag, FLAG purification tag, an…

Expression

Bacterial and Insect

Mutation

Promoter

T7 Promoter

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
IMPT-6213

Plasmid

#99333

Purpose

AT2R N terminal BRIL, linker GSGS, delta aa 1-34, delta aa 336-363

Depositor

Raymond Stevens

Article

Zhang et al Nature. 2017 Apr 20;544(7650):327-332. d

Insert

AGTR2 (AGTR2 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Dll4ICD-HPC4 pBacPAK8.3

Plasmid

#17335

Depositor

Ronald Conaway, Joan Conaway

Article

Koutelou et al J Biol Chem. 2007 Dec 12. ():.

Insert

Delta-like 4 (Dll4 Mouse)

Use

Tags

HPC4

Expression

Insect

Mutation

Promoter

Available since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pOmnibac_ZZ_linker_ybbR_Tau

Plasmid

#159717

Purpose

Expresses tau in Sf9 cells with a ZZ affinity tag and a ybbR tag

Depositor

Ahmet Yildiz

Article

Ferro et al Science. 2022 Jan 21;375(6578):326-331.

Insert

Microtubule-associated protein tau (MAPT Human)

Use

Tags

ZZ affinity tag, ybbR tag

Expression

Insect

Mutation

Promoter

Available since

Jan. 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLfa-PUb-EGFP-3'UTR#5

Plasmid

#176337

Purpose

The synthetic 3'UTR#5 for EGFP expression under the control of the Ae. aegypti polyubiquitin (PUb) promoter

Depositor

Zach Adelman

Article

Chae et al RNA Biol. 2021 Aug 31:1-9. doi: 10.1080/

Insert

3'UTR#5

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW-Nb127D01-mCherry

Plasmid

#171573

Purpose

Expresses mCherry-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-mCherry) in fly cells

Depositor

Hidde Ploegh, Norbert Perrimon

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

Nb127D01-mCherry (CXCR2 Human)

Use

Tags

mCherry

Expression

Insect

Mutation

Promoter

fly actin5C promoter

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW-NbVHH05-mCherry

Plasmid

#171572

Purpose

Expresses mCherry-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-mCherry) in fly cells

Depositor

Hidde Ploegh, Norbert Perrimon

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

NbVHH05-mCherry (UBE2J1 Human)

Use

Tags

mCherry

Expression

Insect

Mutation

Promoter

fly actin5C promoter

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge