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Plasmid#169185PurposeBaculoviral transfer vector to co-express nsp12-His6-3xFlag, nsp7 and nsp8 (SARS-CoV-2) in insect cellsDepositorInsertnsp12-His6-3xFlag/nsp7/nsp8 (ORF1ab Synthetic, SARS-CoV-2)
UseTagsHis6-3xFlag (nsp12 C-terminus)ExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pE6c
Plasmid#17466Purposemodified pENTR vector for C-terminal EYFP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsEYFPExpressionBacterial, Insect, Mammalia…MutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-IRES-let-7 8x
Plasmid#20932DepositorInsert8x let-7 target sites
UseLuciferaseTagsExpressionInsect and MammalianMutation168bp IRES from 5'UTR of reaper gene in Dros…PromoterAvailable sinceJune 19, 2009AvailabilityAcademic Institutions and Nonprofits only -
pOPINHALO7
Plasmid#41117DepositorTypeEmpty backboneUseTagsExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceMarch 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAct-Fok1:dCas9
Plasmid#62211PurposeExpresses Fok1:dCas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous high specificity genome engineering in Drosophila melanogaster.DepositorInsertFok1-dCas9
UseTagsExpressionInsectMutationPromoteract5CAvailable sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pF3BGX-TangoG-lexA
Plasmid#138403PurposeRMCE vector for generating C-terminal Tango fusionDepositorInsertTEVcs(ENLYFQG)-LexAp65
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKF292
Plasmid#72856Purposetemplate base vector for N-terminal tagging, inducible Blasticidin resistanceDepositorInsertbase vector for Nterm-tag templates
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mVenus-PA-Rac1-C450A
Plasmid#22021PurposeExpression of Rac1 fused with light-insensitive LOV2 (C450A mutation)DepositorInsertPA-Rac1-C450A (RAC1 Avena sativa (oat), Human)
UseTags6X His and mVenusExpressionBacterial, Insect, and Mamm…MutationLOV domain contains the light-insensitive C450A m…PromoterCMV, p10Available sinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
AE-Polyubiquitin-GCaMP6s
Plasmid#106868PurposeExpresses polyubiquitin GCaMP6s, a genetically encoded calcium indicator, for live calcium imaging in Aedes aegypti mosquitoes.DepositorInsertAE-Polyubiquitin-GCAaMP6s
UseTagsExpressionInsectMutationPromoterAe. aegypti polyubiquitin (PUb)Available sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
ninaE-dsocs
Plasmid#26857DepositorInsertSlow Termination of Phototransduction (stops Fly)
UseTagsmycExpressionInsectMutationThis STOPS construct contains a mutated/truncated…PromoterAvailable sinceApril 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPac0
Plasmid#12094DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceAug. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
KCNK2
Plasmid#73257PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertKCNK2 (KCNK2 Human)
UseTagsTEV-His10-FLAGExpressionInsectMutationuserdefined:Full_Length 1411PromoterPolyhedrinAvailable sinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAEL003877-Cas9
Plasmid#100581PurposeThe promoter of AAEL003877 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegyptiDepositorInsertsAAEL003877-hspcas9-GFP
Opie-dsRed
UseCRISPRTagsGFP and dsREDExpressionInsectMutationPromoterAAEL003877 and Opie2Available sinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUASP dsRed Rab7 WT
Plasmid#44824DepositorInsertRab7 (Rab7 Fly)
UseDrosophilaTagsdsRedExpressionInsectMutationPromoterAvailable sinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
AT1G35710 X075_pECIA2
Plasmid#115098PurposeBait vector AT1G35710 X075_pECIA2 should be used with prey vector AT1G35710 X075_pECIA14.DepositorInsertAT1G35710 (AT1G35710 Mustard Weed)
UseTagsFc, V5 and hexahistidine tagsExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-His10TEV
Plasmid#159427Purposeempty pFastBac1 vector (polyhedrin promoter) adds TEV-protease-removable N-terminal His10-tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sitesDepositorTypeEmpty backboneUseTagsTEV-protease-cleavable His10-tagExpressionInsectMutationPromoterpolyhedrinAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
10XUAS IVS Jaws mVenus_tr
Plasmid#111553PurposeOptogenetics in Drosophila: UAS-induced expression of Jaws mVenusDepositorInsertJaws_mVenus_tr
UseTagsExpressionInsectMutationwtPromoterhsp70Available sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
AT1G56140 X032_pECIA2
Plasmid#115062PurposeBait vector AT1G56140 X032_pECIA2 should be used with prey vector AT1G56140 X032_pECIA14.DepositorInsertAT1G56140 (AT1G56140 Mustard Weed)
UseTagsFc, V5 and hexahistidine tagsExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
AT1G06840 X062_pECIA2
Plasmid#115052PurposeBait vector AT1G06840 X062_pECIA2 should be used with prey vector AT1G06840 X062_pECIA14.DepositorInsertAT1G06840 (AT1G06840 Mustard Weed)
UseTagsFc, V5 and hexahistidine tagsExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
AT2G19230 M11_pECIA2
Plasmid#114949PurposeBait vector AT2G19230 M11_pECIA2 should be used with prey vector AT2G19230 M11_pECIA14.DepositorInsertAT2G19230 (AT2G19230 Mustard Weed)
UseTagsFc, V5 and hexahistidine tagsExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only