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Plasmid#170143PurposeExpresses residues 1-1899 of CHD7 in insect cellsDepositorInsertchromodomain helicase DNA binding protein 7 (CHD7 Human)
UseTagsFLAGExpressionInsectMutationC-terminal truncation of residues 1900-2997PromoterAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-CHD7 (787-1899)
Plasmid#170145PurposeExpresses residues 787-1899 of CHD7 in insect cellsDepositorInsertchromodomain helicase DNA binding protein 7 (CHD7 Human)
UseTagsFLAGExpressionInsectMutationN-terminal truncation of residues 1-786; C-termin…PromoterAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-CHD7 (787-2138)
Plasmid#170146PurposeExpresses residues 787-2138 of CHD7 in insect cellsDepositorInsertchromodomain helicase DNA binding protein 7 (CHD7 Human)
UseTagsFLAGExpressionInsectMutationN-terminal truncation of residues 1-786; C-termin…PromoterAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-CHD7 (1-1547)
Plasmid#170144PurposeExpresses residues 1-1547 of CHD7 in insect cellsDepositorInsertchromodomain helicase DNA binding protein 7 (CHD7 Human)
UseTagsFLAGExpressionInsectMutationC-terminal truncation of residues 1548-2997PromoterAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-CHD7 (936-2997)
Plasmid#170147PurposeExpresses residues 936-2997 of CHD7 in insect cellsDepositorInsertchromodomain helicase DNA binding protein 7 (CHD7 Human)
UseTagsFLAGExpressionInsectMutationN-terminal truncation of residues 1-935PromoterAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-CHD7 (K999R)
Plasmid#170142PurposeExpresses CHD7 (K999R) in insect cellsDepositorInsertchromodomain helicase DNA binding protein 7 (CHD7 Human)
UseTags6xHis and FLAGExpressionInsectMutationK999RPromoterAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOPINO_F2
Plasmid#171926PurposeBacterial expression of nanobody VHH_F2, which binds to receptor binding domain of the spike protein of SARS-CoV-2DepositorInsertVHH_F2
UseTagsHis6ExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceSept. 23, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pF3BGX-TangoL-lexA
Plasmid#138404PurposeRMCE vector for generating C-terminal Tango fusionDepositorInsertTEVcs(ENLYFQL)-LexAp65
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOCC267
Plasmid#118887Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal HALO tagDepositorInsertNcoI-HIS6-MBP-3C-HALO-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsHIS6-MBP, cleavable with 3C protease; HALO tagExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
nSYB-GAL80-DD
Plasmid#124778PurposeExpresses GAL80 fused with destabilizing domain (DD) downstream of a n-Synaptobrevin promoterDepositorInsertTMP-inducible destabilized GAL80 (GAL80-DD)
UseTagsDDExpressionInsectMutationPromoternSynaptobrevinAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
piB-GFP
Plasmid#13844DepositorInsertTATA-GFP-SV40
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFL-EGFP-3xFlag-DEST
Plasmid#134903PurposeBaculovirus co-expression of GFP and gateway cloned protein with 3x-N-terminal Flag tagDepositorTypeEmpty backboneUseTags3x-FlagExpressionInsectMutationPromoterpolyhedronAvailable sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
57 pLIB_C_CBP
Plasmid#160306PurposeC-term uncleavable CBP tag for insect cell expressionDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterpolyhydrinAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-Nucleocapsid
Plasmid#179696PurposeExpresses H6 tagged SARS CoV-2 nucleocapsid in Sf9 cellsDepositorInsertSARS-CoV-2 Nucleocapsid (N protein) (N Severe acute respiratory syndrome coronavirus 2)
UseTagsHis6-TEV protease siteExpressionInsectMutationPromoterpolyhedrinAvailable sinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQUAST-shibire (ts1)
Plasmid#24362DepositorInsertShibire (ts1) (shi Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pExpreS2-1-GFP
Plasmid#175363PurposeZeoR overexpression of cytosolic GFP in Drosophila ExpreS2ion cellsDepositorInsertsgGFP
UseTagsExpressionInsectMutationF64L, S65C, and I167TPromoterFused Actin-HSP70Available sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCaSpeR-act5cB-GAL4QF
Plasmid#61309PurposeExpresses chimeric transactivator GAL4_DBD:QF2w_AD in DrosophilaDepositorInsertGAL4_DBD::QF2w_AD
UseTagsExpressionInsectMutationChanged EK on C-terminus of QF2 to KKKKPromoterAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQUAST-mtdT-3XHA
Plasmid#24354DepositorInsertmtDT-3XHA
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTIGER_mNeonGreen::3xFLAG::dCrk
Plasmid#131137PurposeUASp construct for over-expression of Drosophila Crk with N-terminal monomeric NeonGreen and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.DepositorInsertCrk (Crk Fly)
UseTags3XFLAG and mNeonGreenExpressionInsectMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFHMSP-LIC-C
Plasmid#26100PurposeDonor vector to generate recombinant baculovirus by site-specific transposition into a baculovirus shuttle vector For use with Bac-to-Bac Expression System insect cells for secreted protein expressionDepositorTypeEmpty backboneUseBaculovirus expressionTags8x His tagExpressionInsectMutationPromoterpolyhedrinAvailable sinceAug. 20, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits