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Plasmid#118909Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal and C-terminal HIS6, cleavable with 3C proteaseDepositorInsertNcoI-HBMss-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C proteaseExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJFRC165-20XUAS-IVS-R::PEST
Plasmid#32142DepositorInsertR::PEST
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pE4c
Plasmid#17464Purposemodified pENTR vector for C-terminal GST tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsGSTExpressionBacterial, Insect, Mammalia…MutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT dati
Plasmid#91800PurposeExpresses the Drosophila dati circular RNADepositorInsertdati (dati Fly)
UseTagsExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_Dual_GST-TEV-EGFP-TBK1
Plasmid#187830PurposePlasmid for the expression and purification of GST-TEV-EGFP-TBK1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1631DepositorInsertTBK1 (TBK1 Human)
UseTagsGST and TEV-mEGFP-GG LinkerExpressionBacterial and InsectMutationPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
His-ZZ-TEV-SNAPf-DYNC2H1
Plasmid#132524PurposeFull length human dynein 2 heavy chain DYNC2H1, Sf9 codon optimised. With 8xHis-ZZ tag, TEV cleavage site, SNAPf tag, precission cleavage site at N-terminus. Assembled from synthesised fragmentsDepositorInsertDYNC2H1 (DYNC2H1 Human)
UseTags8x His, Linker-TEV-linker-TEV, Precission cleavag…ExpressionInsectMutationCodon optimised for Sf9 expressionPromoterAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBPHLWL-GAL4DBD
Plasmid#26256DepositorInsertZIP- Gal4DBD (GAL4 Budding Yeast)
UseDrosophila transgenesisTagsExpressionBacterial and InsectMutationPromoterAvailable sinceFeb. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
pQUAS-DSCP-FLPo
Plasmid#24358DepositorInsertFLPo
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 12, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCoofy28
Plasmid#44004DepositorTypeEmpty backboneUseTagsHis6-GSTExpressionInsectMutationPromoterPPHAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBPGUw-HACK-G4>QF2
Plasmid#80277PurposeGAL4->QF2 HACK construct compatible with GMR attP integrated insertions. Contains 5' and 3' GAL4 homology arms and gRNAs.DepositorInsertQF2
UseTagsExpressionInsectMutationPromoterAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUAST-SIK3 WT
Plasmid#52972Purposeinsect expression of SIK3 WTDepositorInsertSIK3 (Sik3 Fly)
UseTagsHAExpressionInsectMutationPromoterHsp70Available sinceMay 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST-SIK3 T196E
Plasmid#52975Purposeinsect expression of SIK3 T196E constitutively active mutantDepositorInsertSIK3 (Sik3 Fly)
UseTagsHAExpressionInsectMutationT196E constitutively active mutantPromoterHsp70Available sinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-MS2_V
Plasmid#147737PurposeInsect Expression vectorDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LINXb3
Plasmid#73615PurposeLight-induced nuclear export switch for microscopy in mammalian cells.DepositorInsertLINXb3
UseTagsmCherryExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-M13-6His-KIF1C-GFP
Plasmid#130975PurposeExpression of 6His-KIF1C-GFP in insect cells for protein purification.DepositorInsertKIF1C-GFP (KIF1C Human)
UseTags6His and GFPExpressionInsectMutation5 silent mutations that make this construct RNAi …PromoterPolyhedrinAvailable sinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUASP dsRed Rab27 WT
Plasmid#44832DepositorInsertRab27 (Rab27 Fly)
UseDrosophilaTagsdsRedExpressionInsectMutationPromoterAvailable sinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
UAS-7xHalo7::CAAX
Plasmid#87647PurposeFor creating drosophila transgenics expressing UAS-7xHalo7::CAAXDepositorInsert7xHalo7::CAAX
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGE-attB-GMR_mNeonGreen::3XFLAG::dCrk
Plasmid#131143PurposeRescue construct for targeting into crk[ΔattP]. Contains 110bp upstream of transcription start site, mNeonGreen, 3XFLAG, and dCrk cDNA, 3' UTR and 99bp downstream.DepositorInsertmNeonGreen::3XFLAG::Crk (Crk Fly)
UseCre/Lox; Attb recombination siteTags3XFLAG and mNeonGreenExpressionInsectMutationPromoterAvailable sinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-let-7 4x
Plasmid#20930DepositorInsert4x let-7 target sites
UseLuciferaseTagsExpressionInsect and MammalianMutationPromoterAvailable sinceJune 19, 2009AvailabilityAcademic Institutions and Nonprofits only -
20XUAS IVS ChrimsonR mCherry_tr
Plasmid#111546PurposeOptogenetics in Drosophila: UAS-induced expression of ChrimsonR mCherryDepositorInsertChrimsonR_mCherry_trafficked
UseTagsExpressionInsectMutationwtPromoterhsp70Available sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only