Addgene

Showing: 721 - 740 of 4645 results

Results per page:

Sort results by:

pDropIn-T2A-split-intein-Gal4[1-20]-N-int-BlastR

Plasmid

#199197

Purpose

Insert to create split-intein Gal4[N-int] knock-in vectors, with blasticidin resistance, via Drop-In cloning, released via BsmBI digest

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-split-intein-Gal4[1-20]-N-int-BlastR

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDropIn-T2A-split-intein-Gal4[21-881]-C-int-G418R

Plasmid

#199198

Purpose

Insert to create split-intein Gal4[21-881]-C-int knock-in vectors, with G418 resistance, via Drop-In cloning, released via BsmBI digest

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-split-intein-Gal4[21-881]-C-int-G418R

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pUAST>stop>mCD8-GFP

Plasmid

#24385

Depositor

Liqun Luo

Article

Potter et al Volume 141, Issue 3, 536-548, 30 April 2

Insert

mCD8-GFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 5, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc-sgRNA-Cas9.scr_2

Plasmid

#190704

Purpose

Expresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Scrambled sgRNA 2

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Drosophila U6

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Copia Renilla Control

Plasmid

#38093

Depositor

Philip Beachy

Article

Lum et al Science. 2003 Mar 28;299(5615):2039-45.

Insert

renilla luciferase

Use

Luciferase

Tags

Expression

Insect

Mutation

Promoter

copia

Available since

Nov. 12, 2012

Availability

Academic Institutions and Nonprofits only
pKSB-631 U6-sgRNA3/4 AACA GCTT

Plasmid

#173675

Purpose

To clone and express a gRNA in Anopheles

Depositor

Eric Marois

Type

Empty backbone

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCoofy11

Plasmid

#44002

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Use

Tags

His10

Expression

Insect

Mutation

Promoter

hr5

Available since

Nov. 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
53 pLIB_MBP

Plasmid

#160301

Purpose

N-term 3C cleavable MBP tag for insect cell expression

Depositor

John Weir

Article

Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

Type

Empty backbone

Use

Tags

6xHis and MBP

Expression

Insect

Mutation

Promoter

polyhydrin

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDrBB2

Plasmid

#18946

Depositor

Michele Calos

Article

Groth et al Genetics. 2004 Apr . 166(4):1775-82.

Insert

hygromycin gene driven by the copia promoter, phiC31 attB

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
Ac5-STABLE2-puro

Plasmid

#208032

Purpose

Enables expression of 2 ORFs (with or without tags) and selection with puromycin in insect cells. Includes Flag-mCherry-T2A-GFP-T2A-puro with Actin5C promoter.

Depositor

James Sutherland, Rosa Barrio

Article

Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAct:dCas9-VP64

Plasmid

#78901

Purpose

Expresses dCas9-VP64 under actin promoter for SAM CRISPRa in Drosophila cells

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

dCas9-VP64

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

pActin (Drosophila)

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pR I-Ab beta Derp1-117

Plasmid

#113639

Purpose

Insect cell expression vector for I-Ab beta chain fused to Der p 1 117-127 antigenic peptide

Depositor

James Moon

Article

Moon et al Methods Mol Biol. 2018;1799:165-181. doi

Insert

I-Ab beta Derp1-117 (H2-Ab1 Mouse)

Use

Tags

6x His tag, fused to Der p 1 (117-127) peptide vi…

Expression

Insect

Mutation

transmembrane and cytoplasmic domains truncated

Promoter

Metallothionein

Available since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc-y1sgRNA-Cas9

Plasmid

#49331

Purpose

Expresses sgRNA targeting yellow gene and Cas9-Puro in Drosophila S2 cells

Depositor

Ji-Long Liu

Article

Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

Inserts

Cas9
dU6-sgRNA
y1 sgRNA (y Fly)

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Insect

Mutation

Human codon optimised

Promoter

Actin-5c and Drosophila U6

Available since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pTriEx-mCherry-LINXa3-nano

Plasmid

#73616

Purpose

Light-induced nuclear export switch fusion to nano for microscopy in mammalian cells.

Depositor

Brian Kuhlman

Article

Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

Insert

LINXa3-nano

Use

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
UAS-u(S)Cas9

Plasmid

#127383

Purpose

Expresses Cas9 at high level

Depositor

Michael Boutros

Article

Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

Insert

uORF(S)-Cas9

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

UAST

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmARC1_N

Plasmid

#146978

Purpose

Insect Expression of DmARC1

Depositor

Elisa Izaurralde

Insert

DmARC1 (Arc1 Fly)

Use

Tags

Expression

Insect

Mutation

5 silent mutations and one non silent mutation G3…

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLib6.4B-Agam_695

Plasmid

#176668

Purpose

Expression of sgRNA under An. gambiae U6-2 (AGAP013695) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAct-FRT-FRT3-stop-FRT-LexGAD-FRT3-Gal4 attB

Plasmid

#52890

Purpose

"CoinFLP-LexGAD/Gal4" - Expresses LexGAD or Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express LexGAD, or FRT3-FRT3 pair to express Gal4.

Depositor

Iswar Hariharan

Article

Bosch et al Development. 2015 Feb 1;142(3):597-606.

Inserts

LexGAD
Gal4 (GAL4 Budding Yeast)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac HTB DPP9S S730A

Plasmid

#169946

Purpose

Purification of catalytically-dead DPP9S from insect cells

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Use

Tags

HIS-TEV

Expression

Insect

Mutation

S730A

Promoter

polyhedrin

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pQF2wWB

Plasmid

#61313

Purpose

Enhancer trap version of QF2w, analogous to pGAWB

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

QF2w

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge