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Plasmid#62210PurposeExpresses Fok1:dCas9 under control of nanos promoter and 3'UTR. For germ line restricted high specificity genome engineering in Drosophila melanogaster.DepositorInsertFok1-dCas9
UseTagsExpressionInsectMutationPromoternanosAvailable sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB DPP9S
Plasmid#166787PurposeInsect cell expression vector for DPP9S (protein purification)DepositorInsertDPP9 (DPP9 Human)
UseTagsHIS-TEVExpressionInsectMutationPromoterpolyhedrinAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
p2X-UASTattB
Plasmid#189860PurposeFly cloning and transformationDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOPINN-GFP
Plasmid#53541PurposepTirex based GFP fusion vector for expression in mammalian, E. coli and insect cells using the baculovirus systemDepositorInsertGFP
UseTagsN-His-GFPExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDEST-HemmarG
Plasmid#31221DepositorInsertCD4-tdGFP (CD4 Human, Fly, Aequorea victoria)
UseTagsCD4 and EGFP/GFPExpressionInsectMutationFusion of signal peptide of Drosophila Akh gene, …PromoterAvailable sinceOct. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJFRC-20X-UAS-ADARcd
Plasmid#81173Purposeidentification of cell-specific targets of RNA binding proteinsDepositorInsertUAS-ADARcd
UseTagsExpressionInsectMutationPromoterUASAvailable sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_VHH-GFP4::CD8::mCherry
Plasmid#163917PurposeExpression of a membrane-tethered GFP-nanobody marked by mCherry under control of the UAS or LOP enhancersDepositorInsertFusion of vhhGPF4 nanobody with mouse CD8 transmembrane protein and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectMutationPromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
p2Bac.Myc.p44
Plasmid#39220DepositorInsertp44 (GTF2H2 )
UseTagsMycExpressionInsectMutationPromoterPp10Available sinceAug. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUb dPspCas13b + PspCas13b guide RNA
Plasmid#176306PurposeExpresses catalytic dead PspCas13b and its associated guide RNA in Drosophila cellsDepositorInsertPspCas13b
UseTagsHA tagExpressionInsectMutationH133A, H1058APromoterUbi-p63eAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJFRC160-21XUAS-B3RT>-dSTOP-B3RT>-myr::RFP
Plasmid#32139DepositorInsertB3RT> STOP STOP B3RT>
UseTagsExpressionInsectMutationPromoterAvailable sinceNov. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4-Aaeg_774
Plasmid#176659PurposeExpression of sgRNA under Ae. Aegypti U6 (AAEL017774) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprTagsExpressionInsectMutationPromoterAvailable sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUb dRxCas13d + RxCas13d guide RNA
Plasmid#176304PurposeExpresses catalytic dead RxCas13d and its associated guide RNA in Drosophila cellsDepositorInsertRxCas13d
UseTagsHA tagExpressionInsectMutationR239A, H244A, R858A, H863APromoterUbi-p63eAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
UAS-Halo7::CAAX
Plasmid#87645PurposeFor creating drosophila transgenics expressing UAS-Halo7::CAAXDepositorInsertHalo7::CAAX
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
His-ZZ-TEV-BICDR1-GFP
Plasmid#111657PurposeC-terminal GFP tag on BICDR1 for expression in Sf9 cells. 8xHis-ZZ-GFP N-terminal tag, TEV site to cleave 8xHis-ZZ.DepositorInsertBICDR1-GFP (Bicdl1 Mouse)
UseTags8xHis-tag, GFP, TEV site, and ZZExpressionInsectMutationOptimised for Sf9 expressionPromoterAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRB17
Plasmid#52527Purposeserves as template for PCR-mediated fusion of U6-promoter with sgRNADepositorInsertDrosophila U6C promoter
UseCRISPR; Blunt cloning kitTagsT7-promoterExpressionInsectMutationPromoterAvailable sinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pF3BGX-T2A-lexAp65
Plasmid#138393PurposeRMCE vector for generating C-terminal T2A-lexA-p65 fusionDepositorInsertT2A-lexAp65
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Flag-Hel25E WT
Plasmid#110123PurposeExpresses Flag-tagged D. melanogaster Hel25EDepositorInsertHel25E (Hel25E Fly)
UseTagsFlagExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceOct. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Flag-Hel25E K91N
Plasmid#110121PurposeExpresses Flag-tagged D. melanogaster Hel25E with K91N mutation that greatly reduces helicase activityDepositorInsertHel25E (Hel25E Fly)
UseTagsFlagExpressionInsectMutationK91N mutationPromoterMetallothionein Promoter (pMT)Available sinceSept. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Flag-Hel25E D193E
Plasmid#110120PurposeExpresses Flag-tagged D. melanogaster Hel25E with D193E mutation that greatly reduces ATP binding affinityDepositorInsertHel25E (Hel25E Fly)
UseTagsFlagExpressionInsectMutationD193E mutationPromoterMetallothionein Promoter (pMT)Available sinceSept. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTIGER_tdTomato::3xFLAG::dCrk
Plasmid#131138PurposeUASp construct for over-expression of Drosophila Crk with N-terminal tandem Tomato and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.DepositorInsertCrk (Crk Fly)
UseTags3XFLAG and tdTomatoExpressionInsectMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only