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Plasmid#92289PurposePlasmid for expression of NHA-tagged Drosophila melanogaster GW182 CED 7W7A with seven mutated W-motifs in D. melanogaster cellsDepositorInsertdGW182 CED 7W7A (gw Fly)
UseTagsNHAExpressionInsectMutationdGW182 CED: aa 940-1215 with the following point …PromoterAc5Available sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMCh-59
Plasmid#92261PurposePlasmid for expression of HA-tagged Drosophila melanogaster GW182 CED in D. melanogaster cellsDepositorInsertdGW182 (gw Fly)
UseTagsHAExpressionInsectMutationaa 940-1215PromoterAc5Available sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-ATG101-ATG13 HORMA
Plasmid#99332PurposeMammalian expression of one subcomplex from the Human ULK1 complexDepositorUseTagsGSTExpressionInsectMutationPromoterp10 and pHAvailable sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLfa[Tc'hsp5'3'UTR]fa
Plasmid#86450PurposeHeat shock-inducible promoter including hsp-UTRs, ubiquitous expression upon heat-shock of any inserted gene of interest in Tribolium; (Shuttle plasmid)DepositorInsertTc'hsp5'3'UTR
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLfa[UAS-Tc'hsp_p-tGFP-SV40]fa
Plasmid#86452PurposeUAS responder construct for misexpression in Tribolium; UAS drives tGFP expression; (Shuttle plasmid); tGFP can be exchanged by other ORFsDepositorInsertUAS-Tc'hsp_p-tGFP-SV40
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAc-GFP-dSUMOdeltaC
Plasmid#85697Purposeexpression of GFP-SUMO1 delta C in Drosophila cellsDepositorInsertSUMO (Sumo Fly)
UseTagsGFPExpressionInsectMutationnon-conjugatable SUMO, lacking the C-terminal gly…PromoterpAc5Available sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Btk(PH)Akt1(KD)
Plasmid#86591PurposeExpresses chimeric fusion of PH domain of Btk and kinase domain of Akt1DepositorUseTags10xHisExpressionInsectMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt13C(WT)
Plasmid#86576PurposeExpresses cleavable human full-length Akt1(WT)DepositorInsertAkt1 (AKT1 Human)
UseTags10xHisExpressionInsectMutation3C cleavage site inserted at pos. 130PromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-OSF-TRIM5-21R(1-300)
Plasmid#79029PurposeGateway entry vector for cloning and propagation in DH5alphaDepositorInsertTRIM5-21R (1-300)
UseTagsOneSTrEP-FLAGExpressionInsectMutationPromoterT7Available sinceMarch 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-OSF-TRIMCyp (owl monkey)
Plasmid#79035PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertOSF-TRIM5Cyp (owl monkey)
UseTagsOneSTrEP-FLAGExpressionInsectMutationPromoterpolyhedrinAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-OSF-TRIM5alpha(African green monkey pygerythrus)(1-293)
Plasmid#79030PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertOSF-TRIM5alpha(African green monkey pygerythrus)(1-293)
UseTagsOneSTrEP-FLAGExpressionInsectMutationdeleted amino acid 294-515 from frican green monk…PromoterpolyhedrinAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
FoRC.PGm
Plasmid#84586PurposeDrosophila P-element transgenesis vector for cloning rescue transgenes. Cloning site is flanked by FRT sequences and contains constitutive GFP reporter.DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceDec. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAcUW21-hvmeh1
Plasmid#84212Purposebaculovirus transfer vector containing hvmeh1 coding sequence from Heliothis virescentDepositorInsertmeh1
UseTagsExpressionInsectMutationPromoterp10Available sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAcUW21-CqJHE
Plasmid#84213Purposebaculovirus transfer vector containing cqjhe coding sequence from Culex quinquefasiatusDepositorInsertJHE
UseTagsExpressionInsectMutationPromoterp10Available sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAcUW21-hovimeh1
Plasmid#84211Purposebaculovirus transfer vector containing hovimeh1 coding sequence from Homalodisca vitripennisDepositorInsertmeh1
UseTagsExpressionInsectMutationPromoterp10Available sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
exon K-renilla
Plasmid#81007Purposeluciferase based mini-gene to report splicing at exon 25 in Drosophila paraylticDepositorInsertparalytic (para Fly)
UseTagsRenilla luciferaseExpressionInsectMutationspanning exon 24 to exon 26, termination codon in…PromoterP-AC5 (Actin 5C)Available sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
UAS-Xbp1spliced
Plasmid#80628Purposeexpression of Xbp1 RB isoform in DrosophilaDepositorInsertXbp1 (Xbp1 Fly)
UseTagsExpressionInsectMutationRB isoformPromoterhsp70Available sinceJuly 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSGE_975_Nhe2_EcR-mut
Plasmid#71179PurposeLuciferase reporterDepositorInsert975_Nhe2_EcR-mut
UseLuciferaseTagsluciferaseExpressionInsectMutationPromoterDSCP Drosophila synthetic core promoterAvailable sinceMay 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSGE_717_S2-1_CGCG-to-DFD
Plasmid#71174PurposeLuciferase reporterDepositorInsert717_S2-1_CGCG-to-DFD
UseLuciferaseTagsluciferaseExpressionInsectMutationPromoterDSCP Drosophila synthetic core promoterAvailable sinceMay 10, 2016AvailabilityAcademic Institutions and Nonprofits only -