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Plasmid#110119PurposeExpression plasmid for expressing circular RNAs of a desired sequence in Drosophila using the dati flanking introns to drive backsplicingDepositorInsertdati (dati Fly)
UseTagsExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT dati Exons 1-3
Plasmid#110113PurposeDrosophila dati minigene plasmid that expresses both a linear RNA and a circular RNADepositorInsertdati (dati Fly)
UseTagsExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT dati MCS circfirefly_1100
Plasmid#110117PurposeExpresses a 1100-nt firefly luciferase circular RNA in Drosophila cellsDepositorInsertFirefly luciferase
UseTagsExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT dati MCS circfirefly_900
Plasmid#110116PurposeExpresses a 900-nt firefly luciferase circular RNA in Drosophila cellsDepositorInsertFirefly luciferase
UseTagsExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT dati MCS circfirefly_700
Plasmid#110115PurposeExpresses a 700-nt firefly luciferase circular RNA in Drosophila cellsDepositorInsertFirefly luciferase
UseTagsExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT dati MCS circfirefly_500
Plasmid#110114PurposeExpresses a 500-nt firefly luciferase circular RNA in Drosophila cellsDepositorInsertFirefly luciferase
UseTagsExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGS_N_Strep
Plasmid#101651PurposeExpression of type I signal-peptide containing proteins with N-terminal Twin-Strep-tag in insect cellsDepositorInsertLRP6 Signal peptide, KpnI linker, Twin-Strep tag
UseTagsTwin-Strep tagExpressionInsectMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGS_C_Strep
Plasmid#101652PurposeExpression of type I signal-peptide containing proteins with C-terminal Twin-Strep-tag in insect cellsDepositorInsertLRP6 Signal peptide, MCS linker, Twin-Strep tag
UseTagsTwin-Strep tagExpressionInsectMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGS_C_3xFLAG
Plasmid#101653PurposeExpression of type I signal-peptide containing proteins with C-terminal 3xFLAG-tag in insect cellsDepositorInsertLRP6 Signal peptide, MCS linker, 3xFLAG tag
UseTags3xFLAG tagExpressionInsectMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-3xVAS1-Syn21-Citrine-HA-P10-3xVAS4-Syn21-FLAG-Cerulean-P10
Plasmid#104619PurposeExpresses HA tagged Citrine or FLAG tagged Cerulean upon binding of TALE1 to VAS1 or TALE4 to VAS4DepositorInsert3xVAS4-hsp70-Syn21-FLAG-Cerulean-P10
UseTagsFLAGExpressionInsectMutationPromoterhsp70Available sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-3xVAS4-Syn21-FLAG-Cerulean-P10
Plasmid#104618PurposeExpresses FLAG tagged Cerulean upon binding of TALE4 to VAS4DepositorInserthsp70-Syn21-FLAG-mCerulean-P10
UseTagsFLAGExpressionInsectMutationPromoterhsp70Available sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-3xVAS4-IVS-Syn21-GFP-P10
Plasmid#104615PurposeExpresses GFP upon binding of TALE4 to VAS4DepositorInsert3xVAS4
UseTagsExpressionInsectMutationPromoterhsp70Available sinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-3xVAS1-Syn21-Citrine-HA-P10
Plasmid#104616PurposeExpresses HA tagged Citrine upon binding of TALE1 to VAS1DepositorInsertSyn21-Citrine-HA-P10-MhcSD
UseTagsHAExpressionInsectMutationPromoterhsp70Available sinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJC-20xUAS-TALE-VP64-P10
Plasmid#104605PurposeControl plasmid (no TALE array) with P10 terminatorDepositorTypeEmpty backboneUseTALENTagsExpressionInsectMutationPromoterhsp70Available sinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-AthHEN1-CM
Plasmid#104981PurposeExpression of FLAG Myc tagged catalytic dead A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila S2 cellsDepositorInsertHen1 (HEN1 Mustard Weed)
UseTagsFLAG MycExpressionInsectMutationPromoterActin5CAvailable sinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUAST-Myc-mSesn2 (1186)
Plasmid#66920Purposeexpression of mouse Sesn2 in drosophilaDepositorInsertSesn2 (Sesn2 Mouse)
UseTagsMycExpressionInsectMutationPromoterHS promoterAvailable sinceDec. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMCh-623
Plasmid#92266PurposePlasmid for expression of NHA-tagged Drosophila melanogaster GW182 6W6A with six mutated W-motifs in its N-terminal domain in D. melanogaster cellsDepositorInsertdGW182 6W6A (gw Fly)
UseTagsNHAExpressionInsectMutationThe following point mutations were introduced W23…PromoterAc5Available sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMCh-695
Plasmid#92263PurposePlasmid for expression of NHA-tagged Drosophila melanogaster GW182 with mutated PAM2 motif in D. melanogaster cellsDepositorInsertdGW182 PAM2 mut (gw Fly)
UseTagsNHAExpressionInsectMutationThe following point mutations were introduced: EF…PromoterAc5Available sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pMCh-76
Plasmid#92288PurposePlasmid for expression of NHA-tagged Drosophila melanogaster GW182 CED in D. melanogaster cellsDepositorInsertdGW182 CED (gw Fly)
UseTagsNHAExpressionInsectMutationdGW182 CED: aa 940-1215PromoterAc5Available sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only