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Plasmid#118872Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal monomeric GFPDepositorInsertNcoI-HIS6-3C--NotI-ccdB-AscI-mGFP-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C protease and mGFPExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC 3GLA
Plasmid#71762Purposegap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, Amp resistantDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSGE_886_Nhe2_EcR-to-ato_RB
Plasmid#71181PurposeLuciferase reporterDepositorInsert886_Nhe2_EcR-to-ato_RB
UseLuciferaseTagsluciferaseExpressionInsectMutationPromoterDSCP Drosophila synthetic core promoterAvailable sinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pFL His-I3 V41A / W43A
Plasmid#113515PurposeExpression of His-I3 V41A / W43 A in insect cells (Baculovirus system)DepositorInsertI3 (PPP1R11 Human)
UseTags6*HisExpressionInsectMutationV41A /W43APromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFL His-I3
Plasmid#113513PurposeExpression of His-I3 in insect cells (Baculovirus system)DepositorInsertI3 (PPP1R11 Human)
UseTags6*HisExpressionInsectMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVL-FLAG/His LCOR
Plasmid#114450PurposeInsect baculovirus expression vector containing N terminal FLAG/His tagged human LCOR coding sequenceDepositorInsertLCOR (LCOR Human)
UseTagsFLAG-HisExpressionInsectMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-GST-Mps1-KD
Plasmid#114066Purposeprotein productionDepositorInsertMps1-KD (TTK Human)
UseTagsGSTExpressionInsectMutationMps1 KD (D664A)PromoterAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-GST-Mps1-WT
Plasmid#114065Purposeprotein productionDepositorInsertMps1-WT (TTK Human)
UseTagsGSTExpressionInsectMutationnonePromoterAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOEM1_pCMV:axFgf8-pPH:vsvged
Plasmid#111159PurposeBaculovirus rescue vector, VSVGED pseudotype, hShhDepositorInsertaxFgf8
UseBaculoviral rescue shuttle vectorTagsExpressionInsect and MammalianMutationwtPromoterCMVAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-LacI-LAP-HEC1
Plasmid#114033PurposeProduction of baculovirus with LacI-LAP-HEC1DepositorInsertLacI-LAP-HEC1 (NDC80 Human)
UseTagsExpressionInsectMutationnonePromoterCMVAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual PNG172-STII PLU-HIS
Plasmid#112992PurposeBaculovirus expression of Drosophila png172-Flag and plu-HisDepositorUseTagsFlag and HisExpressionInsectMutationpng172 mutationPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual PNG-STII PLU-HIS
Plasmid#112993PurposeBaculovirus expression of Drosophila png-Flag and plu-HisDepositorUseTagsFlag and HisExpressionInsectMutationPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOEM1_pCMV:deltaN-bmp4-pPH:vsvged
Plasmid#111158PurposeBaculovirus rescue vector, VSVGED pseudotype, hShhDepositorInsertdeltaNhBmp4 (BMP4 Human)
UseBaculoviral rescue shuttle vectorTagsExpressionInsect and MammalianMutationdelta N, mutant BMP lacking the N terminus (delta…PromoterCMVAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUAST-attB-Flag-dTSC2 S1338A
Plasmid#111808PurposeInsect Expression of dTSC2DepositorInsertdTSC2 (TSC2 Fly)
UseTags3xFlagExpressionInsectMutationS1338APromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
WNK3 (WNK3B-c005)
Plasmid#110262PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertWNK3 (WNK3 Human)
UseTagsHis-TEVExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
WNK3 (WNK3B-c001)
Plasmid#110261PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertWNK3 (WNK3 Human)
UseTagsHis-TEVExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
PKN2 (PRKCL2A-c038)
Plasmid#110265PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertPRKCL2 (PKN2 Human)
UseTagsHis6-ZBasic-TEVExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
MTHFR (MTHFRA-c042)
Plasmid#110271PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertMTHFR (MTHFR Human)
UseTagsTEV-His10-FlagExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
LIMK2 (LIMK2A-c052)
Plasmid#110264PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertLIMK2 (LIMK2 Human)
UseTagsHis-TEVExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only