-
Plasmid#146071PurposeInsect Expression of DmTral-LSmR21EE23KR42EF44ADepositorInsertDmTral-LSmR21EE23KR42EF44A (tral Fly)
UseTagsExpressionInsectMutationR21E, E23K, R42E, F44A. Additionally, one silent …PromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-R42EF44A_C
Plasmid#146061PurposeInsect Expression of DmTral-R42EF44ADepositorInsertDmTral-R42EF44A (tral Fly)
UseTagsExpressionInsectMutationR42E, F44A. Additionally, two silent mutations A1…PromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-LSm-R21EE23KS13AI15A-V5His6_C
Plasmid#146049PurposeInsect Expression of DmTral-LSmR21EE23KS13AI15ADepositorInsertDmTral-LSmR21EE23KS13AI15A (tral Fly)
UseTagsExpressionInsectMutationS13A, I15A, R21E, E23K, one silent mutation A198C…PromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmTral-FDF-ADA_C
Plasmid#146039PurposeInsect Expression of DmTral-FDF-ADADepositorInsertDmTral-FDF-ADA (tral Fly)
UseTagsExpressionInsectMutationFDF to ADA (407-409) mutation. Additionally, thre…PromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-Q22YU3 del C3-2xStrep
Plasmid#170316PurposePlasmid for insect cell expression of Tetrahymena thermophila Q22YU3 with a C-terminal truncation (C3-finger). Tagged with 2x Strep-tag II at the C-terminus.DepositorInsertQ22YU3 C-terminal truncation (Delta-C3)
UseTagsStrep-Tag IIExpressionInsectMutationDeletion of C-terminal C3-fingerPromoterpolyhedrinAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-Q22MS1-2xStrep
Plasmid#170314PurposePlasmid for insect cell expression of Tetrahymena thermophila Q22MS1 C-terminally tagged with 2x Strep-tag IIDepositorInsertQ22MS1
UseTagsStrep-Tag IIExpressionInsectMutationPromoterPolyhedrinAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1C-5Box-7-Fluc-STOP-CG10011-SV40_D
Plasmid#146156PurposeInsect Expression of 5BoxB-7-Fluc-CG10011-3UTRDepositorInsert5BoxB-7-Fluc-CG10011-3UTR
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmMe31B_268-459_D
Plasmid#146119PurposeInsect Expression of DmMe31B_268-459DepositorInsertDmMe31B_268-459 (me31B Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-DmPABPC1-V5His6_D
Plasmid#146095PurposeInsect Expression of DmPABPC1DepositorInsertDmPABPC1 (pAbp Fly)
UseTagsExpressionInsectMutationseven silent mutations compared to the sequence g…PromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVMG0193_Cq-Rpl40-Cas9
Plasmid#169346PurposeExpression of Cas9 in Culex quinquefasciatus or other mosquitoesDepositorInsertpVMG0193_Cq-Rpl40-Cas9
UseCRISPRTagsExpressionInsectMutationPromoterCPIJ002413Available sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVMG0217_Cq-U6-2b_2xBbsI-gRNA
Plasmid#169339PurposeExpression of gRNA in Culex quinquefasciatus or other mosquitoesDepositorInsertpVMG0217_Cq-U6-2b_2xBbsI-gRNA
UseCRISPRTagsExpressionInsectMutationPromoterCPIJ039728Available sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmSmaug_763-999-V5His6_C
Plasmid#146013PurposeInsect Expression of DmSmaug_763-999DepositorInsertDmSmaug_763-999 (smg Fly)
UseTagsExpressionInsectMutation2 silent mutations and a deletion of the AA 961-9…PromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-R42E_B
Plasmid#145988PurposeInsect Expression of DmTral-R42EDepositorInsertDmTral-R42E (tral Fly)
UseTagsExpressionInsectMutationtwo silent mutations A198C, A387G and a mutation …PromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmSmaug_1-762-V5His6_C
Plasmid#146001PurposeInsect Expression of DmSmaug_1-762DepositorInsertDmSmaug_1-762 (smg Fly)
UseTagsV5-6xHisExpressionInsectMutationNP_523987.1, 7 silent mutations compared to the s…PromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmZuc_B
Plasmid#145949PurposeInsect Expression of DmZucDepositorInsertDmZuc (zuc Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmExu_B
Plasmid#145939PurposeInsect Expression of DmExuDepositorInsertDmExu (exu Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-F629V_A
Plasmid#145898PurposeInsect Expression of DmAGO1-F629VDepositorInsertDmAGO1-F629V (AGO1 Fly)
UseTagsExpressionInsectMutationV315A, F629V, H913R - variation with 2 non silent…PromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmLSm3_A
Plasmid#145888PurposeInsect Expression of DmLSm3DepositorInsertDmLSm3 (LSm3 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmDCP2_209-791_A
Plasmid#145848PurposeInsect Expression of DmDCP2_209-791DepositorInsertDmDCP2_209-791 (DCP2 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1_GI.1_Q62C-A140C
Plasmid#162581PurposeExpresses norovirus GI.1 VP1 protein with mutations Q62C-A140C in Sf9 insect cellsDepositorInsertVP1 protein from human norovirus GI.1
UseTagsExpressionInsectMutationGlutamine 62 changed to Cysteine and Alanine 140 …PromoterpolyhedringAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only