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Plasmid#170807PurposeExpresses Msp300 KASH domain fused to 2xFLAG and mCherry under Gal4/UAS control for Drosophila transgenesisDepositorInsert2xFLAG/mCherry-Msp300KASH
UseTags2xFLAG-mCherry and Msp300KASHExpressionInsectMutationPromoter5xUAS + hsp70 minimal promoterAvailable sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-20XUAS-IVS-Syn21-op1-jGCaMP7c-T2A-tdTomato-p10
Plasmid#172914PurposeGal4 activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP7c-T2A-tdTomato
UseTags6xHis and T2A-tdTomatoExpressionInsectMutationPromoterhsp70Available sinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-20XUAS-IVS-Syn21-op1-jGCaMP7b-T2A-tdTomato-p10
Plasmid#172913PurposeGal4 activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP7b-T2A-tdTomato
UseTags6xHis and T2A-tdTomatoExpressionInsectMutationPromoterhsp70Available sinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAW-BiP-Nb127D01-mCherry-KDEL
Plasmid#171575PurposeExpresses mCherry-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-mCherry) in fly cell ER membraneDepositorInsertBiP-Nb127D01-mCherry-KDEL (CXCR2 Human)
UseTagsBiP signal peptide and mCherry-KDELExpressionInsectMutationPromoterfly actin5C promoterAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFASTbac-HisGST-PTPIP51(36-470)
Plasmid#170531PurposeExpresses Cleavable HisGST-tagged PTPIP51 (36-470) in Sf9DepositorInsertProtein tyrosine phosphatase interacting protein 51 (RMDN3 )
UseTagsGST tag and His tagExpressionInsectMutationPromoterPolyhedrinAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-Q22YU3-2xStrep
Plasmid#170315PurposePlasmid for insect cell expression of Tetrahymena thermophila Q22YU3 C-terminally tagged with 2x Strep-tag IIDepositorInsertQ22YU3 (TTHERM_00122270 Tetrahymena thermophila)
UseTagsStrep-Tag IIExpressionInsectMutationPromoterPolyhedrinAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Mdn1-deltaC
Plasmid#171603PurposeExpresses S. pombe Mdn1 a.a. 1-3911 in insect cells.DepositorInsertMdn1
UseTagsHis6 tagExpressionInsectMutationC-terminal deletion; a.a. 1-3911 onlyPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
Mdn1-FL
Plasmid#171602PurposeExpresses full length S. pombe Mdn1 in insect cells.DepositorInsertMdn1
UseTagsHis6 tagExpressionInsectMutationPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral_394-543-V5His6_D
Plasmid#146134PurposeInsect Expression of DmTral_394-543DepositorInsertDmTral_394-543 (tral Fly)
UseTagsExpressionInsectMutationtwo silent mutations (nucleotides A198C, A387G) a…PromoterAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmBelle_1-298_B
Plasmid#145913PurposeInsect Expression of DmBelle_1-298DepositorInsertDmBelle_1-298 (bel Fly)
UseTagsExpressionInsectMutationdel 158-160 (GGG)PromoterAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1C-Fluc-CG7785_A
Plasmid#145863PurposeInsect Expression of CG7785-3UTRDepositorInsertCG7785-3UTR (CG7785 Fly)
UseTagsExpressionInsectMutation317bp deletion in 3'UTR region (4172-4273bp)…PromoterAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBIG1b_nsp7 (SARS-CoV-2)
Plasmid#169178PurposeBaculoviral transfer vector to express untagged nsp7 (SARS-CoV-2) in insect cellsDepositorInsertnsp7 (ORF1ab SARS-CoV-2, Synthetic)
UseTagsuntaggedExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBIG1c_nsp8 (SARS-CoV-2)
Plasmid#169179PurposeBaculoviral transfer vector to express untagged nsp8 (SARS-CoV-2) in insect cellsDepositorInsertnsp8 (ORF1ab SARS-CoV-2, Synthetic)
UseTagsuntaggedExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBIG1b_nsp10-6His-3xFlag (SARS-CoV-2)
Plasmid#169162PurposeBaculoviral transfer vector to express SARS-CoV-2 nsp10 in insect cellsDepositorInsertnsp10-6His-3xFlag (ORF1ab SARS-CoV-2, Synthetic)
UseTags6His-3xFlagExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBIG1a_3xFlag-6His-nsp14 (SARS-CoV-2)
Plasmid#169163PurposeBaculoviral transfer vector to express SARS-CoV-2 nsp14 in insect cellsDepositorInsert3xFlag-6His-nsp14 (ORF1ab SARS-CoV-2, Synthetic)
UseTags3xFlag-6HisExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAW-BiP-NbVHH05-mCherry-KDEL
Plasmid#171574PurposeExpresses mCherry-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-mCherry) in fly cell ER membraneDepositorInsertBiP-NbVHH05-mCherry-KDEL (UBE2J1 Human)
UseTagsBiP signal peptide and mCherry-KDELExpressionInsectMutationPromoterfly actin5C promoterAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1C-Fluc-nerfin_E
Plasmid#146190PurposeInsect Expression of nerfin-3UTRDepositorInsertnerfin-3UTR (nerfin-1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmUPF2-siRNAres-V5His6_E
Plasmid#146166PurposeInsect Expression of DmUPF2-siRNAresDepositorInsertDmUPF2-siRNAres (Upf2 Fly)
UseTagsExpressionInsectMutation3 non silent mutations: L1022P, V1097L and N1213S…PromoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFL GST-UBXN2A
Plasmid#169016PurposeExpresses GST-UBXN2A (human) in insect cellsDepositorInsertUBXN2A (UBXN2A Human)
UseTagsGSTExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAC-U63-tgRNA-Gal80
Plasmid#169030PurposegRNA-marker vector contain a U6:3 promoter, gRNA scaffold, and a Ubi-Gal80 marker.DepositorTypeEmpty backboneUseCRISPRTagsExpressionInsectMutationPromoterU6:3Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only