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pAc5.1B-lambdaN-HA-HsAGO2-F2V2_F

Plasmid

#146286

Purpose

Insect Expression of HsAGO2-F2V2

Depositor

Elisa Izaurralde

Article

Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

Insert

HsAGO2-F2V2 (AGO2 Human)

Use

Tags

Expression

Insect

Mutation

Contains F470V, F505V, K660E mutations compared t…

Promoter

Available since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmHelz-E958Q_AH

Plasmid

#148884

Purpose

Insect Expression of DmHelz-E958Q

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

DmHelz-E958Q (CG9425 Fly)

Use

Tags

Expression

Insect

Mutation

two silent an two non silent G1374S, R2076G mutat…

Promoter

Available since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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pExpreS2-1-CR-PA

Plasmid

#175445

Purpose

HiFi-compatible destination vector for secreted overexpression of thrombin-cleavable, C-terminal Protein A tagged proteins with ExpreS2 Platform

Depositor

Wyatt Yue

Article

Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

Type

Empty backbone

Use

Tags

BiP Secretion Peptide and Thrombin-Protein A

Expression

Insect

Mutation

Promoter

Fused Actin-HSP70

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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BicC.Ca9.R

Plasmid

#168295

Purpose

Expresses Cas9 in Drosophila late germline cells

Depositor

Omar Akbari

Article

Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

Insert

BicC.Cas9-T2A-eGFP-p10

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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1137B=Hsp70Bb-Cas9

Plasmid

#153284

Purpose

The attB plasmid harboring the Hsp70Bb-Cas9-T2A-eGFP-p10 and a mini-white marker.

Depositor

Omar Akbari

Article

Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

Insert

pHsp70Bb-SpCas9-T2A-eGFP-p10 (cas9 Synthetic)

Use

CRISPR

Tags

eGFP

Expression

Insect

Mutation

Promoter

Dmel Hsp70Bb

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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1046E=dgRNAs[traB,bTub]

Plasmid

#149425

Purpose

The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes.

Depositor

Omar Akbari

Article

Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

Insert

U6.3-gRNAs[TraB, bTub]

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

U6.3

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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1098E=TI-pgSIT[tra,bTub,Hasp70Bb-Cas9]

Plasmid

#149427

Purpose

The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.

Depositor

Omar Akbari

Article

Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

Inserts

U6.3-gRNAs[TraB, bTub]
Hsp70Bb-Cas9-T2A-eGFP-p10

Use

CRISPR

Tags

eGFP

Expression

Insect

Mutation

Promoter

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p{ie1-DsRed::FRT,attB}

Plasmid

#182190

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p{3P3-eGFP::GW,attB}

Plasmid

#182167

Purpose

fluorescent reporter gene with intron containing Gateway cloning cassette

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

EGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p{3P3-dsRed::GW,attB}

Plasmid

#182168

Purpose

fluorescent reporter gene with intron containing Gateway cloning cassette

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p{ie1-DsRed::GW,attB}

Plasmid

#182173

Purpose

fluorescent reporter gene with intron containing Gateway cloning cassette

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p{ie1-eGFP::GW,attB}

Plasmid

#182172

Purpose

fluorescent reporter gene with intron containing Gateway cloning cassette

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

eGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p{Mhc-F4-678-DsRed::GW,attB}

Plasmid

#182171

Purpose

fluorescent reporter gene with intron containing Gateway cloning cassette

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p{Mhc-F4-678-DsRed::FRT-RC,attB}

Plasmid

#182187

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p{3P3-dsRed::KD,attB}

Plasmid

#182178

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p{3P3-dsRed::FRT,attB}

Plasmid

#182176

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p{Mhc-F4-678-eGFP::FRT,attB}

Plasmid

#182185

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

EGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p{ie1-DsRed::FRT-RC,attB}

Plasmid

#182191

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p{Mhc-F4-678-GFP::KD-RC,attB}

Plasmid

#182184

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

EGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmHelz_1213-2103_AH

Plasmid

#148883

Purpose

Insect Expression of DmHelz_1213-2103

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

DmHelz_1213-2103 (CG9425 Fly)

Use

Tags

Expression

Insect

Mutation

two silent an two non silent G1374S, R2076G mutat…

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge