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Plasmid#146702PurposeInsect Expression of DmNot1_1710-2505DepositorInsertDmNot1_1710-2505 (Not1 Fly)
UseTagsExpressionInsectMutationtwo non silent mutations (A1747T, G1999S) and one…PromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmCup-Y342AL347A_O
Plasmid#147103PurposeInsect Expression of DmCup-Y342AL347ADepositorInsertDmCup-Y342AL347A (cup Fly)
UseTagsExpressionInsectMutationY342A, L347A, I350L, P418S, K1045NPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmHPat-dsRNAres_J
Plasmid#146692PurposeInsect Expression of DmHPat-dsRNAresDepositorInsertDmHPat-dsRNAres (Patr-1 Fly)
UseTagsExpressionInsectMutationtwo non silent, A479V, G568D and one silent mutat…PromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1-2mut-F1389A_R
Plasmid#147394PurposeInsect Expression of CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1-2mut-F1389ADepositorInsertCeAIN2_254-400-HsTNRC6C_1207-1690-CIM1-2mut-F1389A (TNRC6C Nematode, Human)
UseTagsExpressionInsectMutationDeletion of 2 AA (SD) at position 378-379 compare…PromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1A-DmXRN1-D207A-dsRNAres-V5His6_O
Plasmid#147065PurposeInsect Expression of DmXRN1-D207A-dsRNAresDepositorInsertDmXRN1-D207A-dsRNAres (pcm Fly)
UseTagsExpressionInsectMutationone silent mtuation compared to the sequence give…PromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1A-DmXRN1-del1323-1355-dsRNAres-V5His6_N
Plasmid#146969PurposeInsect Expression of DmXRN1-del1323-1355-dsRNAresDepositorInsertDmXRN1-del1323-1355-dsRNAres (pcm Fly)
UseTagsExpressionInsectMutationone silent mtuation compared to the sequence give…PromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGW182-delCterm_E
Plasmid#146252PurposeInsect Expression of DmGW182-delC-termDepositorInsertDmGW182-delC-term (gw Fly)
UseTagsExpressionInsectMutationV758A, Q817R, and N853SPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmSMG6-siRNAres-V5His6_E
Plasmid#146241PurposeInsect Expression of DmSMG6-siRNAresDepositorInsertDmSMG6-siRNAres (Smg6 Fly)
UseTagsExpressionInsectMutationT151A, deleted A947PromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAct-GST-H3(K27Q)
Plasmid#182843Purposea K27Q point mutation (AAG/CAG) of Drosophila histone H3 was generated from pAct-GST-H3. Expression in S2 cells.DepositorInsertGST-histone H3 fusion
UseTagsGSTExpressionInsectMutationPromoterActin5CAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAct-GST-H3
Plasmid#182842PurposeA PCR product encoding GST-H3 (Drosophila histone H3) was was inserted into a modified pAct-5c vector by Sac I and Sal I sites. Expression in S2 cells.DepositorInsertGST-histone H3 fusion
UseTagsGSTExpressionInsectMutationPromoterActin5CAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMT-FLAG-CBPΔ(N+HAT)
Plasmid#182841PurposeAn EcoRV fragment (encoding residues 1566-2142) was removed from pMT-FLAG-CBPΔN. Expression in S2 cells by CuSO4 induction.DepositorInsertCBP (w Fly)
UseTagsFLAG tagExpressionInsectMutationdletion of residues 1566-2142 from CBP1476-3222PromoterMTAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMT-FLAG-CBPΔN
Plasmid#182840PurposeA 5.2 kb Drosophila CBP cDNA fragment (cut by Nhe I and Nsi I), encoding C-terminal residues 1476-3222, was inserted into the pMT-FLAG vector. Expression in S2 cells by CuSO4 induction.DepositorInsertCBP cDNA fragment (cut by Nhe I and Nsi I) (nej Fly)
UseTagsFLAG tagExpressionInsectMutationPromoterMTAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCasper-pNos-MCP-mCherry
Plasmid#180275Purposeto epress MCP-mCherry fusion protein in the early fly embryos (driven by maternal nanos driver)DepositorInsertMCP-mCherry
UseTagsmCherryExpressionInsectMutationPromoternanosAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
p{3P3-mTurquoise::FRT,attB}
Plasmid#182180Purposefluorescent reporter gene with yeast recombination site within intronDepositorInsertmTurquoise
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
p{Mhc-F4-678-EGFP::GW,attB}
Plasmid#182170Purposefluorescent reporter gene with intron containing Gateway cloning cassetteDepositorInsertEGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
p{3P3-mTurquoise::GW,attB}
Plasmid#182169Purposefluorescent reporter gene with intron containing Gateway cloning cassetteDepositorInsertmTurquoise
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
3xTRE DREaMR 3p3DsR
Plasmid#182206Purpose3xTRE Tet-On cassette with 3xp3 DsRed maker for generating knockins with doxycycline-inducible rescueDepositorInsertrTetR-3XP3-DsRed
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
1xTRE DREaMR 3p3DsR
Plasmid#182204Purpose1xTRE Tet-On cassette with 3xp3 DsRed marker for generating knockins with doxycycline-inducible rescue.DepositorInsertrTetR-3XP3-DsRed
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmDCP2-PB-V5His6_G
Plasmid#146359PurposeInsect Expression of DmDCP2-PBDepositorInsertDmDCP2-PB (DCP2 Fly)
UseTagsExpressionInsectMutationContains an in-frame insertion that adds a Q afte…PromoterAvailable sinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-HsAGO2_E
Plasmid#146224PurposeInsect Expression of HsAGO2DepositorInsertHsAGO2 (AGO2 Human)
UseTagsExpressionInsectMutationContains a K660E mutation compared to the sequenc…PromoterAvailable sinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only