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Plasmid#186664PurposeNbr C-tag sgRNA2 plasmidDepositorInsertNbr sgRNA 2 Plasmid (Nbr Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
Nbr_C_sgRNA1
Plasmid#186663PurposeNbr C-tag sgRNA1 plasmidDepositorInsertNbr sgRNA 1 Plasmid (Nbr Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
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Cas9_nonPuro_OSS
Plasmid#186652PurposeCas9 plasmid to express the Cas9 in OSS cellsDepositorInsertOSS Cas9 Expression Plasmid
UseTagsExpressionInsectMutationPromoterAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
puc19_N_tag
Plasmid#186650PurposeEmpty donor plasmid.DepositorInsertN-terminal 3xFlag-3xHA Tag
UseTags3xFlag-3xHAExpressionInsectMutationPromoterAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
PUC19_C_tag
Plasmid#186659PurposeEmpty donor plasmid for C-terminal tag of 3xFlag-3xHA.DepositorInsertC-terminal 3xFlag-3xHA Tag
UseTags3xFlag-3xHAExpressionInsectMutationPromoterAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDonor_N_FH_GLE
Plasmid#186657PurposeDonor plasmid to endogenously tag GLE gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.DepositorInsertOSS Gle N-terminal 3xFlag-3xHA Tag Donor Plasmid (CG14749 Fly)
UseTags3xFlag-3xHAExpressionInsectMutationPromoterAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pBig1a zz TEV YBBR POT1 MBP TEV TPP1 MBP TEV TIN2 ZZ TEV TRF2 (4comp2)
Plasmid#185448PurposeCoexpresses human POT1 with a zz affinity tag, YBBR site, and TEV site, human TRF2 with a zz tag and TEV site, and human TIN2 and TPP1 each with an MBP affinity tag and TEV site in insect cellsDepositorUseTagsMBP, YBBR, and ZZExpressionInsectMutationSee Depositor Comments BelowPromoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOmniBac zz TEV YBBR TRF1
Plasmid#185445PurposeExpresses human TRF1 with a zz affinity tag, YBBR labeling site, and TEV cleavage site in insect cellsDepositorInsertTRF1 (TERF1 Human)
UseTagsYBBR and ZZExpressionInsectMutationPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
FAK Fus
Plasmid#186145PurposeFor expresseion of recombinant FAK using baccolovirus in SF9 cellsDepositorInsertPTK2 (PTK2 Mouse)
UseTagsHis6ExpressionInsectMutationC-terminal linker swapped with FUS IDRPromoterpolyhedrin promoterAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-M13-6His-KIF1C_P176L-GFP
Plasmid#185978PurposeBaculovirus expression of human KIF1C with P176L mutation and 6xHis and GFP tags.DepositorInsertKIF1C (KIF1C Human)
UseTags6xHis and eGFPExpressionInsectMutationP176L - mutation causing HSP (SPG58)PromoterAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBac FAK W266A
Plasmid#186144PurposeFor expresseion of recombinant FAK using baccolovirus in SF9 cellsDepositorInsertPTK2 (PTK2 Mouse)
UseTagsHis6ExpressionInsectMutationDNA mutation T796G;G797C Amino acid mutation W266APromoterpolyhedrin promoterAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
p130Cas Y15F
Plasmid#186154PurposeFor expressing recombinant p130Cas in insect cells.DepositorInsertBCAR1 (Bcar1 Rat)
UseTagsHis6 (Tev)ExpressionInsectMutation15Y residues mutated to AlaninePromoterpolyhedrinAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
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FAK Pyk2
Plasmid#186146PurposeFor expresseion of recombinant FAK using baccolovirus in SF9 cellsDepositorInsertPTK2 (PTK2 Mouse)
UseTagsHis6ExpressionInsectMutationC-terminal linker swapped with Pyk2 linkerPromoterpolyhedrin promoterAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
BMP10 N67Q N131Q D4G (S2-2)
Plasmid#184622Purposefor transfection into S2 cells and expression of BMP10 proteinDepositorInsertBMP10 (BMP10 Human)
UseTags8x His - streptavidin binding peptideExpressionInsectMutationN67Q, N131Q, ARIRR(312-316) replaced with GPromoterAvailable sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBig2ab zz TEV YBBR POT1 ZZ TEV TPP1 MBP TEV TIN2 ZZ TEV TRF1 (4comp1)
Plasmid#185447PurposeCoexpresses human POT1 with a zz affinity tag, YBBR site, and TEV site, human TRF1 and TPP1 each with a zz tag and TEV site, and human TIN2 with an MBP affinity tag and TEV site in insect cellsDepositorUseTagsMBP, YBBR, and ZZExpressionInsectMutationPromoterAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056D
Plasmid#183132PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii bTub, Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[bTub]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056E
Plasmid#183133PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii dsx, Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[dsx]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only