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Plasmid#192470PurposeTransient expression of RvCAHS8 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cellsDepositorInsertCAHS8
UseTagsAcGFP1ExpressionInsectMutationPromoterAc5 promoterAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1/V5-HisA-RvCAHS3-GS-AcGFP1
Plasmid#192469PurposeTransient expression of RvCAHS3 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cellsDepositorInsertCAHS3
UseTagsAcGFP1ExpressionInsectMutationPromoterAc5 promoterAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGBdest-10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1_F32A-I35A-I36A_-GFP-TEV-StrepII
Plasmid#192705PurposePlasmid for the Expression of the E3 complex with an FII (F32A, I35A, and I36A) mutation. SMC Internal reference: SMC1728DepositorUseTags-GFP-TEV-StrepII and 10x-His-TEVExpressionInsectMutationF32A-I35A-I36APromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[R272A,K276A, R280A,K284A]
Plasmid#188599PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler (Nbr Fly)
UseTagsFLAG-MycExpressionInsectMutationR272A [CGG=> GCG], K276A [AAG=>GCG], R280A …PromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
PRE-Hsp70BbCas9_1.2
Plasmid#190796PurposegypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with one gypsy insulator element. Step 2 in cloning process.DepositorInsertgypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
PRE-Hsp70BbCas9_1.0
Plasmid#190795PurposePRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements. Step 1 in cloning processDepositorInsertPRE-Hsp70Bb-Cas9-T2A-eGFP
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
PALI1_PIR_pFB1
Plasmid#191249PurposeExpresses PALI1 from insect cellsDepositorInsertLCoR
UseTagsMBPExpressionInsectMutationNAPromoterPolyhedrinAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
MON +T111H
Plasmid#187432PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationchanged threonine at 117 to histidinePromoterPH and p10Available sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[K216E]
Plasmid#188600PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler (Nbr Fly)
UseTagsFLAG-MycExpressionInsectMutationK216E [AAA=> GAA]PromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[C399-625 CM]
Plasmid#188588PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler C-terminal domain, catalytic mutant
UseTagsFLAG-MycExpressionInsectMutationD435A [GAT=>GCC]; E435A [GAA=>GCC]PromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[C399-625]-Gwpep(Mm)
Plasmid#188589PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertGW-peptide (mouse) C-terminally tagged C-terminal domain of Nibbler
UseTagsFLAG-Myc and GWpep mmExpressionInsectMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[K276A]
Plasmid#188597PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler (Nbr Fly)
UseTagsFLAG-MycExpressionInsectMutationK276A [AAG=>GCG]PromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[C399-625]-Gwpep(Dm)
Plasmid#188590PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertGW-peptide (drosophila) C-terminally tagged C-terminal domain of Nibbler
UseTagsFLAG-Myc and Gwpep dmExpressionInsectMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Gwpep(Dm)-Nbr[C399-625]
Plasmid#188592PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertGW-peptide (drosophila) N-terminally tagged C-terminal domain of Nibbler
UseTagsFLAG-Myc-Gwpep dmExpressionInsectMutationPromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Gwpep(Mm)-Nbr[C399-625]
Plasmid#188591PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertGW-peptide (mouse) N-terminally tagged C-terminal domain of Nibbler
UseTagsFLAG-Myc-Gwpep mmExpressionInsectMutationPromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[R272A,K276A]
Plasmid#188596PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler (Nbr Fly)
UseTagsFLAG-MycExpressionInsectMutationR272A [CGG=> GCG], K276A [AAG=>GCG]PromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[K284A]
Plasmid#188595PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler (Nbr Fly)
UseTagsFLAG-MycExpressionInsectMutationK284A [AAG=> GCG]PromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[R272A]
Plasmid#188594PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler (Nbr Fly)
UseTagsFLAG-MycExpressionInsectMutationR272A [CGG=> GCG]PromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[K187A]
Plasmid#188593PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler (Nbr Fly)
UseTagsFLAG-MycExpressionInsectMutationK187A [AAA=> GCA]PromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr-CM
Plasmid#188585PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler Catalytic mutant
UseTagsFLAG-MycExpressionInsectMutationdeleted first ATG; D435A [GAT=>GCC]; E435A [GA…PromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only