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Plasmid#201552PurposeExpresses Octopus kinesin-1 motor with K282R mutation with HaloTag in Drosophila S2 cellsDepositorInsertKinesin-1
UseTagsHaloTagExpressionInsectMutationchanged lysine 282 to arginine (numbering based o…PromoterAc5Available sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-bullfrog saxiphilin
Plasmid#140595Purposeprotein expressionDepositorInsertsaxiphilin bullfrog
UseTagsGFP-His10ExpressionInsectMutationDeletion of M20 - please see depositor commentPromoterpolyhedrin promoterAvailable sinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
LRP1A-c004
Plasmid#205112PurposeLRP1 protein expressionDepositorInsertLRP1 (LRP1 Human)
UseTagsAviTag and GP64sp-6His-TEVExpressionInsectMutationWTPromoterAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
QPRTA-c002
Plasmid#205115PurposeQPRT protein expressionDepositorInsertQPRT (QPRT Human)
UseTagsTEV-6HisExpressionInsectMutationWTPromoterAvailable sinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
Ac5-STABLE1-blast
Plasmid#208033PurposeEnables expression of an ORF (with or without tags) and selection with blasticidin in insect cells. Includes GFP-T2A-blast with Actin5C promoter.DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAttB-Tub-HA-Wnk
Plasmid#203968PurposeFly transformation vector expressing indicated Wnk variant under tubulin promoter for ubiquitous expressionDepositorInsertWnk (most similar to RO variant) (Wnk Fly)
UseTagsHAExpressionInsectMutationPromoterAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAttB-Tub-HA-WnkDeltaNT
Plasmid#203969PurposeFly transformation vector expressing indicated Wnk variant under tubulin promoter for ubiquitous expressionDepositorInsertWnk (Wnk Fly)
UseTagsHAExpressionInsectMutationNterm deletionPromoterAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAttB-Tub-HA-WnkDeltaAI
Plasmid#203970PurposeFly transformation vector expressing indicated Wnk variant under tubulin promoter for ubiquitous expressionDepositorInsertWnk (Wnk Fly)
UseTagsHAExpressionInsectMutationdeletion of autoinhibitory domainPromoterAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAttB-Tub-HA-WnkDeltaMID
Plasmid#203971PurposeFly transformation vector expressing indicated Wnk variant under tubulin promoter for ubiquitous expressionDepositorInsertWnk (Wnk Fly)
UseTagsHAExpressionInsectMutationdeletion of middle partPromoterAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAttB-Tub-HA-WnkDeltaCC
Plasmid#203972PurposeFly transformation vector expressing indicated Wnk variant under tubulin promoter for ubiquitous expressionDepositorInsertWnk (Wnk Fly)
UseTagsHAExpressionInsectMutationdeletion of coiled coil domains 1 and 2PromoterAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAttB-Tub-HA-WnkDeltaCC1
Plasmid#203973PurposeFly transformation vector expressing indicated Wnk variant under tubulin promoter for ubiquitous expressionDepositorInsertWnk (Wnk Fly)
UseTagsHAExpressionInsectMutationdeletion of coiled coil domain 1PromoterAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAttB-Tub-HA-WnkDeltaCC2
Plasmid#203974PurposeFly transformation vector expressing indicated Wnk variant under tubulin promoter for ubiquitous expressionDepositorInsertWnk (Wnk Fly)
UseTagsHAExpressionInsectMutationdeletion of coiled coil domain 2PromoterAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAttB-Tub-HA-WnkDeltaCT
Plasmid#203975PurposeFly transformation vector expressing indicated Wnk variant under tubulin promoter for ubiquitous expressionDepositorInsertWnk (Wnk Fly)
UseTagsHAExpressionInsectMutationC-terminal deletion after RFxV motifPromoterAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pAMW-WnkAAxV
Plasmid#203980PurposeMyc6 Wnk under actin promoter for cell cultureDepositorInsertWnk (Wnk Fly)
UseTags6xMycExpressionInsectMutationRFxV mutated to AAxVPromoterAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
H3-pAc5.1 OST4 mCherry Oskar 139-606
Plasmid#206494PurposeOskar 139-606 expression for Schneider cell imagingDepositorInsertOskar 139-606
UseTagsOST4 mcherryExpressionInsectMutationPromoterAvailable sinceOct. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUAS-kkv-OH
Plasmid#138956PurposeUsed to generate a transgene that will express an epitope tagged Chitin Synthase in Drosophila using Gal4DepositorArticleInsertkkv with C terminal epitope tag (kkv Fly)
UseTagsollas and his-6 added to C terminus of protein co…ExpressionInsectMutationPromoterUASAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCFD3-dUG-DH1
Plasmid#138963PurposeUsed to edit the endogenous Drosophila kkv gene to encode a fluorescent Chitin Synthase.DepositorArticleInsertDH1 oligo
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMMK ENTR 3 polH aeBlue polH VSV-G
Plasmid#206278PurposeENTR Vector 3 for MultiSite Gateway assembly. Encodes aeBlue chromoprotein and VSV-G glycoprotein under the control of viral polH promoters.DepositorInsertaeBlue, VSV-G
UseMultimate/gateway entr 3TagsExpressionBacterial and InsectMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only