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Plasmid#206471PurposeBruno expression in Schneider cells for imagingDepositorInsertBruno (aret Fly)
UseTagsPH mcherryExpressionInsectMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-(FspAI)-mCherry-PH (EB3)
Plasmid#206472PurposeExpression in Schneider cells for imagingDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-CCR4 isoform A-mCherry-PH (EB7)
Plasmid#206473PurposeCCR4 expression in Schneider cells for imagingDepositorInsertCCR4 (twin Fly)
UseTagsmcherry PHExpressionInsectMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-OST4-mCherry (EcoRV) (XH26)
Plasmid#206475PurposeExpression in Schneider cells for imagingDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-OST4-mCherry-CAF1 isoform A (HK184)
Plasmid#206476PurposeCAF1 expression in Schneider cells for imagingDepositorInsertCAF1 (Pop2 Fly)
UseTagsOST4 mcherryExpressionInsectMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac dual_Human full length _CDK7 (S164D, T170E)_del327-346
Plasmid#217012PurposeExpression of CDK7 in insect cellsDepositorInsertCDK7 (S164D, T170E)_del327-346 (CDK7 Human)
UseTagsTEV cleavable his tagExpressionInsectMutation(S164D, T170E)_del327-346PromoterAvailable sinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac dual_Human full length CDK7(W132R, S164D, T170E)
Plasmid#217013PurposeExpression of CDK7 in insect cellsDepositorInsertCDK7(W132R, S164D, T170E) (CDK7 Human)
UseTagsTEV cleavable his tagExpressionInsectMutationCDK7(W132R, S164D, T170E)PromoterAvailable sinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
T5-pAc5.1 EGFP-EcoRV
Plasmid#206481PurposeSchneider cell imagingDepositorTypeEmpty backboneUseTagsEGFPExpressionInsectMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
TH1274-054_Jie_EWSR1_pOCC177_pOEM1-N-HIS-MBP-PS-NotI-AscI-TEV-SNAP
Plasmid#221896PurposeShuttle vector for baculovirus production, using FlashBac bacmidDepositorInsertEWSR1 (EWSR1 Human)
UseTags6xHis-MBP-PreScission and TEV-SNAP-tagExpressionInsectMutationwtPromoterPH promoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
TH0994-pOCC177-Fus-wt_(1-211)
Plasmid#221886PurposeShuttle vector for baculovirus production, using FlashBac bacmidDepositorInsertFUS (FUS Human)
UseTags6xHis-MBP-PreScission and TEV-SNAP-tagExpressionInsectMutationPLD domain (aa 1-211)PromoterPH promoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
TH1148-pOCC177-FUS(Nhe)-PLD_YtoF
Plasmid#221887PurposeShuttle vector for baculovirus production, using FlashBac bacmidDepositorInsertFUS (FUS Human)
UseTags6xHis-MBP-PreScission and TEV-SNAP-tagExpressionInsectMutationIn PLD all Tyr (Y) changed to Phe (F)PromoterPH promoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
TH1149-pOCC177-FUS(Nhe)-RGG_RtoK
Plasmid#221888PurposeShuttle vector for baculovirus production, using FlashBac bacmidDepositorInsertFUS (FUS Human)
UseTags6xHis-MBP-PreScission and TEV-SNAP-tagExpressionInsectMutationIn RGG (aa 212-526) most of Arg (R) changed to Ly…PromoterPH promoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
TH1203-pOCC177_pOEM1-N-HIS-MBP-PS-TAF15_opt-TEV-SNAP
Plasmid#221891PurposeShuttle vector for baculovirus production, using FlashBac bacmidDepositorInsertTAF15 (TAF15 Human)
UseTags6xHis-MBP-PreScission and TEV-SNAP-tagExpressionInsectMutationwtPromoterPH promoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
TH1202-pOCC175_pOEM1-N-HIS-MBP-PS-mGFP-TEV-HNRNPA3_opt
Plasmid#221890PurposeShuttle vector for baculovirus production, using FlashBac bacmidDepositorInsertHNRNPA3 (HNRNPA3 Human)
UseTags6xHis-MBP-PreScission-mGFP-TEVExpressionInsectMutationwtPromoterPH promoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
C1C2CMUNC2C
Plasmid#218674PurposeInsect cell expression of a rat MUNC13-1 fragment containing domains C1C2BMUNC2CDepositorInsertMUNC13 (Unc13a Rat)
UseTagsGSTExpressionInsectMutationAmino acids1407-1453 deletedPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-GST-TBK1
Plasmid#221331PurposeTBK1 protein overexpression in Sf9 insect cellsDepositorInsertTBK1 (Tbk1 Mouse)
UseTags6xHis-TEV-2xGST-TEVExpressionInsectMutationPromoterpolyhedrinAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
TH1263-018_Jie_SFPQ_pOCC119_pOEM1-N-HIS6-MBP-NotI-AscI-C-TEV-mGFP
Plasmid#221895PurposeShuttle vector for baculovirus production, using FlashBac bacmidDepositorInsertSFPQ (SFPQ Human)
UseTags6xHis-MBP-PreScission and TEV-mGFPExpressionInsectMutationwtPromoterPH promoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiT-GEM(2)
Plasmid#220448PurposeSplit intein version of Trojan-Gal4 Expression Module in Phase 2. Insert T2A-Gal4 to genomic loci using Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exonsDepositorInsertCfaN-Gal4-CfaC
UseTagsExpressionInsectMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiTE(2)-Gal4
Plasmid#220451PurposeSplit intein version of Trojan-Gal4. Create Gal4 driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 2 coding intron.DepositorInsertCfaN-Gal4-CfaC
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB-6HZB
Plasmid#218680PurposeExpression of Z-tagged proteins using BaculovirusesDepositorTypeEmpty backboneUsePfbTagsHis-Z-TEVExpressionInsectMutationPromoterPolyhedrinAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only