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Plasmid#217896PurposeInsect cell expression of human 20S CP proteasome subunit beta type-5 (PSMB5), beta type-6 (PSMB6), and beta type-7 (PSMB7) with C-terminal Twin-Strep-tagDepositorUseTags2xSTIIExpressionInsectMutationPromoterAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only
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pFastbac-Flag-BRCA1
Plasmid#223228PurposeInsect expression construct for Flag-tagged BRCA1 protein production and purificationDepositorInsertBreast Cancer 1 (BRCA1 Human)
UseTagsFlagExpressionInsectMutationPromoterPolyhedrinAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiT-GEM(0)
Plasmid#220446PurposeSplit intein version of Trojan-Gal4 Expression Module in Phase 0. Insert T2A-Gal4 to genomic loci using Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exonsDepositorInsertCfaN-Gal4-CfaC
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-PH-mCherry-MARF1 isoform D (HK56)
Plasmid#206470PurposeMARF1 expression in Schneider cells for imagingDepositorInsertMARF1 (CG17018 Fly)
UseTagsPH mcherryExpressionInsectMutationPromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSDC803_IKZF1_mutant
Plasmid#224113PurposeInsect cell expression vector for IKZF1 mutant for CRBN cryoEM pre-blottingDepositorInsertIKZF1 (IKZF1 Human)
UseTagsFlag-TEVExpressionInsectMutationResidues 140-196, Q146A and G151NPromoterpolyhedrinAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB His-TEV-rDPP9(S729A)-FLAG
Plasmid#217329PurposePurification of catalytically-dead rat DPP9 fusion with C-terminal FLAG tagfrom insect cellsDepositorInsertDPP9
UseTagsFLAG and His-TEVExpressionInsectMutationS729APromoterpolyhedrinAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB His-TEV-rDPP9-FLAG
Plasmid#217328PurposePurification of rat DPP9 fusion with C-terminal FLAG tag from insect cellsDepositorInsertDPP9
UseTagsFLAG and His-TEVExpressionInsectMutationPromoterpolyhedrinAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB His-TEV-rDPP9(S729A)
Plasmid#217327PurposePurification of catalytically-dead rat DPP9 from insect cellsDepositorInsertDPP9
UseTagsHis-TEVExpressionInsectMutationS729APromoterpolyhedrinAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB His-TEV-rDPP9
Plasmid#217326PurposePurification of rat DPP9 from insect cellsDepositorInsertDPP9
UseTagsHis-TEVExpressionInsectMutationPromoterpolyhedrinAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB His-TEV-BN-rNLRP1(N61K)-FLAG
Plasmid#217325PurposePurification of Brown Norway rat NLRP1 (N61K mutation) fusion with C-terminal FLAG tag from insect cellsDepositorInsertNLRP1
UseTagsFLAG and His-TEVExpressionInsectMutationN61KPromoterpolyhedrinAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB His-TEV-BN-rNLRP1(S969A)-FLAG
Plasmid#217324PurposePurification of autocleavage-deficient Brown Norway rat NLRP1 fusion with C-terminal FLAG tag from insect cellsDepositorInsertNLRP1
UseTagsFLAG and His-TEVExpressionInsectMutationS969APromoterpolyhedrinAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB His-TEV-BN-rNLRP1-FLAG
Plasmid#217323PurposePurification of Brown Norway rat NLRP1 fusion with C-terminal FLAG tag from insect cellsDepositorInsertNLRP1
UseTagsFLAG and His-TEVExpressionInsectMutationPromoterpolyhedrinAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB His-TEV-LEW-rNLRP1(S969A)-FLAG
Plasmid#217321PurposePurification of autocleavage-deficient Lewis rat NLRP1 fusion with C-terminal FLAG tag from insect cellsDepositorInsertNLRP1
UseTagsFLAG and His-TEVExpressionInsectMutationS969APromoterpolyhedrinAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB His-TEV-LEW-rNLRP1-FLAG
Plasmid#217320PurposePurification of Lewis rat NLRP1 fusion with C-terminal FLAG tag from insect cellsDepositorInsertNLRP1
UseTagsFLAG and His-TEVExpressionInsectMutationPromoterpolyhedrinAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUAST-3x attB
Plasmid#220329Purposeincludes 3 standard UAS cassettesDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
2xUAS-1xhsp70-attB
Plasmid#220317Purposeincludes 2 UAS cassettes and 1 heat-shock inducible hsp70 casetteDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAceBAC2-MLG-HMF-LST2
Plasmid#220787PurposeExpresses N-terminal His10-Myc-FLAG-LST2 in insect cellsDepositorArticleInsertLST2 (ZFYVE28 Human)
UseTags10xHis-Myc-FLAGExpressionInsectMutationPromoterp10Available sinceAug. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
hs-cre::EBD, UAS-dBrainbow attB
Plasmid#220326Purposeexpresses a heat-shock inducible cre::EBD fusion protein and UAS-dBrainbowDepositorInsertsCre::EBD (with intron)
dBrainbow
UseTagsExpressionInsectMutationPromoterUAS and hsAvailable sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
tRECs/3xUAS-FP attB
Plasmid#220330Purposeencodes three Fluorescent Protein FLP-OUT cassettes responsive to three different recombinases (mFLP5, B3 and KD)DepositorInsertsB3RT-STOP-B3RT-TagBFP-3xHA
KDRT-STOP-KDRT-GFP-HSV
FRTm71-STOP-FRTm71-mCherry
UseTagsExpressionInsectMutationPromoterUASAvailable sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
Vector 1174K
Plasmid#221017PurposeFluorescent based sex separator in Anopheles species mosquitoes (SEPARATOR)DepositorInsertEngineered dobulesex splicing module (Anopheles gambiae)
UseSynthetic BiologyTagsExpressionInsectMutationPromoterAvailable sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only