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Plasmid#224269PurposeProtein purification of Rac1 (G12C)DepositorInsertRac1 (RAC1 Human)
UseTagsExpressionBacterialMutationG12CPromoterAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pProEx M-Ras(G22C) CL
Plasmid#224268PurposeProtein purification of M-Ras (G22C)DepositorInsertM-Ras (MRAS Human)
UseTagsExpressionBacterialMutationG22CPromoterAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pProEx Rit1(G47C) CL
Plasmid#224267PurposeProtein purification of Rit1 (G47C)DepositorInsertRit1 (RIT1 Human)
UseTagsExpressionBacterialMutationG47CPromoterAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pProEx Ypt1(S17C) CL
Plasmid#224265PurposeProtein purification of Ypt1 (S17C)DepositorInsertYpt1 (YPT1 Budding Yeast)
UseTagsExpressionBacterialMutationS17CPromoterAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pProEx Rap1A(G12C, L96F) CL
Plasmid#224264PurposeProtein purification of Rap1A (G12C, L96F)DepositorInsertRap1A (RAP1A Human)
UseTagsExpressionBacterialMutationG12C, L96FPromoterAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pProEx RalA(G23C) CL
Plasmid#224263PurposeProtein purification of RalA (G23C)DepositorInsertRalA (RALA Human)
UseTagsExpressionBacterialMutationG23CPromoterAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pProEx N-Ras(G12C) CL
Plasmid#224262PurposeProtein purification of N-Ras (G12C)DepositorInsertN-Ras (NRAS Human)
UseTagsExpressionBacterialMutationG12CPromoterAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pProEx H-Ras(G12C) CL
Plasmid#224261PurposeProtein purification of H-Ras (G12C)DepositorInsertH-Ras (HRAS Human)
UseTagsExpressionBacterialMutationG12CPromoterAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1-His-TEV-OTC_Ub
Plasmid#172134PurposeScaffold design for studying ubiquitin interacting proteins by cryo-EM.DepositorInsertE. coli ornithine transcarbamylase (OTC), fused to human ubiquitin
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-FLAG-SpCas9-HF1
Plasmid#126770PurposeExpression of increased fidelity SpCas9-HF1 in bacterial cellsDepositorInsertSpCas9-HF1
UseCRISPRTags3xFLAG, 6xHis, MBP, and NLSExpressionBacterialMutationN497A, R661A, Q695A, Q926APromoterT7Available sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gαi3-LgB91
Plasmid#134359PurposeNanoluc complementation assay. Expression of Gαi3 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi3. Addition of the HA epitope at N terminus of Gαi3.DepositorInsertGαi3-LgB91 (GNAI3 Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
DiLiCre 2.0
Plasmid#198752PurposeA Cre recombinase that is expressed upon doxycycline when combined with the UbC-blasticidine-P2A-TETon-3G transactivator. DiLiCre 2.0 is a cre recombinase that is activated by violet light.DepositorInsertCre-PhoCI-ERT2-ERT2
UseLentiviralTagsCre, kept in a photocleavable site, followed by 2…ExpressionBacterial and MammalianMutationPromoterTRE3GVAvailable sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET23a-mUbc9
Plasmid#53137PurposeE.coli expression of full length untagged mouse Ubc9 (protein sequence identical to human)DepositorInsertUbc9 (Ube2i Mouse)
UseTagsuntaggedExpressionBacterialMutationprotein sequence is identical to humanPromoterT7Available sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX Syt1 C2AB (96-421) D303G
Plasmid#170646PurposeExpression of syt1 for purification in E. ColiDepositorInsertSYT1 (Syt1 Rat)
UseTagsExpressionBacterialMutationD303GPromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX Syt1 C2AB (96-421) D365E
Plasmid#170649PurposeExpression of syt1 for purification in E. ColiDepositorInsertSYT1 (Syt1 Rat)
UseTagsExpressionBacterialMutationD365EPromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX Syt1 C2AB (96-421) I367T
Plasmid#170652PurposeExpression of syt1 for purification in E. ColiDepositorInsertSYT1 (Syt1 Rat)
UseTagsExpressionBacterialMutationI367TPromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
SB_convex
Plasmid#110104PurposepBXPHM3 with scaffold Sybody of the convex libraryDepositorInsertscaffold Sybody of the convex library
UseTagsHisExpressionBacterialMutationPromoterPBADAvailable sinceMay 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAB46 (CK2beta)
Plasmid#27085DepositorInsertCK2beta (CSNK2B Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceMay 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_PRMT1-Halo
Plasmid#175334PurposeProduction of HaloTag fusion proteinDepositorInsertPRMT1 (PRMT1 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
His-SUMO- flag-hDDI2-D252N_pET11a
Plasmid#169123Purposeexpression of proteinDepositorInserthDDI (DDI2 Human)
UseTagsExpressionBacterialMutationD252NPromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only