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Plasmid#120873PurposeExpresses E. coli codon optimized Cas12c2 effector in the pET28a backbone.DepositorInsertCas12c2
UseCRISPRTagsmH6ExpressionBacterialMutationPromoterlacAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET22b-T5-sfGFP*- MmH-MH4R
Plasmid#196409PurposeExpress sfGFP with TAG and enzymes for biosynthesizing DOPADepositorInsertsUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTn7CdrA-gfp(ASV)C
Plasmid#111617PurposeFluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.DepositorInsertPcdrA-RBSII-gfp(ASV)-T0-T1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X1 Flag WDR5
Plasmid#15553DepositorInsertWdr5 (Wdr5 Mouse)
UseTagsFlag and GSTExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X1 Flag RbBP5
Plasmid#15551DepositorInsertRbBP5 (RBBP5 Human)
UseTagsFlag and GSTExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
Sfp pet29b C-terminal His Tag
Plasmid#75015PurposeExpresses the PPTase Sfp from B. subtilis; loads CoA analogues onto ACPDepositorInsertSfp
UseTagsHis TagExpressionBacterialMutationPromoterT7Available sinceMay 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyCatcher
Plasmid#223633PurposeProtein purification of SpyCatcher003 (S49C) with 6xHis tag from E. coliDepositorInsertSpyCatcher003 S49C
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNO286-3
Plasmid#107746PurposeKey genes: "mini-CcaS #10", ho1, pcyA, spectinomycin resistance, and medium copy backbone (p15a)DepositorInsertsCcaS
Ho1
PcyA
UseSynthetic BiologyTagsExpressionBacterialMutationDeleted AA 223 and 512PromoterJ23106 and J23108Available sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mOct4 Full length(FL)
Plasmid#206394PurposeExpresses Oct4 with a 6xHis tag for protein purificationDepositorInsertOct4 (Pou5f1 Mouse)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pBbE7c-RFP
Plasmid#35314DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X-1-Full-Length-MePCE
Plasmid#113539PurposeProduces an N-terminal GST-tagged full length MePCE protein.DepositorInsertMePCE (MEPCE Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCHARGE3
Plasmid#24658DepositorInsertTurbo
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 9, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X1 Flag hDPY30
Plasmid#15556DepositorInsertDPY30 (DPY30 Human)
UseTagsFlag and GSTExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
RCAS(B)-Rheb-Q64L
Plasmid#21050DepositorInsertRas-homolog enriched in brain (RHEB Human)
UseRetroviralTagsFLAGExpressionBacterialMutationglutamine 64 to leucinePromoterAvailable sinceAug. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBSTNAV
Plasmid#45801DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-APOBEC1-YTH
Plasmid#200158PurposeFor bacterial expression of the APOBEC1-YTH fusion protein for protein purificationDepositorInsertAPOBEC1-YTH
UseTags6xHis, HA, and Maltose Binding ProteinExpressionBacterialMutationCodon optimized for E coli expressionPromoterLacZAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCR-Zeo
Plasmid#158709PurposeFor cloning and constitutive expression of crRNAs in mycobacteriumDepositorInsertcrRNA
UseCRISPR and Synthetic Biology; Mycobacteria expres…TagsExpressionBacterialMutationPromoterHsp60Available sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET30a-His6-ABP-LOXCAT
Plasmid#139971PurposeFusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2. Contains a albumin binding peptide (ABP).DepositorInsertA fusion of lactate oxidase from A. viridans and catalase from E. coli
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -