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Showing: 161 - 180 of 28286 results

  5. pBHM2403

    Plasmid
    #171687
    Purpose
    pRS316-PTEF1-CnoCas9, contains Cryptococcus neoformans codon optimized Cas9 under the control of the TEF1 promoter and terminator.
    Depositor
    Hiten Madhani
    Article
    Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi
    Insert
    CnoCAS9
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    TEF1
    Available since
    May 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    171687-plasmid-map-sequence-id-340174

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  11. pBHM2329

    Plasmid
    #171686
    Purpose
    pRS316-PCnU6-scaffold, contains template for amplification of PCnU6 and scaffold for fusion PCR
    Depositor
    Hiten Madhani
    Article
    Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi
    Insert
    PCnU6-sgRNA_scaffold
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    C. neoformans U6
    Available since
    May 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    171686-plasmid-map-sequence-id-340161

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  17. pET28a-HaloTag7SS-DogTag

    Plasmid
    #171775
    Purpose
    Expresses in bacterial cytoplasm HaloTag7 with DogTag inserted between D139 and E140, as well as C61S and C261S in HaloTag7 to block disulfide bond formation
    Depositor
    Mark Howarth
    Article
    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-
    Insert
    HaloTag7SS-DogTag
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    DogTag inserted in HaloTag7 between residues D139…
    Promoter
    T7
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171775-plasmid-map-sequence-id-338524

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  19. G3BP1A

    Plasmid
    #42480
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    G3BP1A
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. Twin-Strep(R)-Tev-GSGS-Ubiquitin

    Plasmid
    #186803
    Purpose
    Codon-optimized bacterial expression plasmid. Expresses Twin-Strep(R)-Tev-GSGS-Ubiquitin.
    Depositor
    Lalit Deshmukh
    Article
    Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/
    Insert
    Twin-Strep(R)-Tev-GSGS-Ubiquitin
    Use
    Tags
    GS-Strep (WSHPQFER)-GGGSGGGSGGSSA-Strep (WSHPQFER…
    Expression
    Bacterial
    Mutation
    Strep tag Lysine's mutated to Arginine
    Promoter
    Available since
    March 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    186803-plasmid-map-sequence-id-372592

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Showing: 161 - 180 of 28286 results