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Plasmid#84693PurposeProtein expression in mycobacteriaDepositorTypeEmpty backboneUseTagsHis6ExpressionBacterialMutationPromoterAcetamidaseAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only
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pET-28c(+)-YTHDF2
Plasmid#102275PurposeFor bacterial expression of His-tagged human YTHDF2DepositorInsertYTHDF2 (YTHDF2 Human)
UseTags6X HISExpressionBacterialMutationPromoterT7Available sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
nuc-mCherry-pTRIP
Plasmid#163520PurposeUsed to construct lentivirus to express nucleus-targeted mCherry in mammalian cellsDepositorInsertnucleus targeted mCherry
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET14b – lambda exonuclease
Plasmid#104531Purposebacterial expression of lambda exonucleaseDepositorInsertλ exonuclease
UseTagsHis6 followed by a thrombin siteExpressionBacterialMutationPromoterT7Available sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOPINS3C
Plasmid#41115DepositorTypeEmpty backboneUseTagsExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceMarch 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMMBGent
Plasmid#45475DepositorInsertGentamycin
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRSET-mSA-EGFP
Plasmid#39862DepositorInsertmonomeric streptavidin fused to EGFP
UseTags6xHis, EGFP, and FLAGExpressionBacterialMutationPromoterT7Available sinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRSETB PKA RIalpha
Plasmid#14922DepositorInsertPKA R1alpha regulatory subunit (PRKAR1A Bovine)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2007AvailabilityAcademic Institutions and Nonprofits only -
His-DERA
Plasmid#203225PurposeFor protein expression. E. coli 2-deoxy-D-ribose-5-phosphate aldolase. Converts dR5P to G3P and acetaldehyde. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.DepositorInsertdeoxyribose aldolase
UseTagsN-terminal TEV cleavable 6xHis tagExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Pet22b-mEGFP
Plasmid#166439PurposeBacterial expression of 6x His tagged mEGFPDepositorInsertmEGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pY002 (pFnCpf1_min)
Plasmid#69975PurposeExpresses FnCpf1 and spacers 1-4 of CRISPR array under artificial promoter. Cas genes are removed.DepositorInsertFnCpf1 locus without Cas genes nd artificial promoter
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCCM
Plasmid#162708PurposeSecond CCM operon cloned from H. neapolitanus; pFA expressing the remaining 11 genes in the H. neapolitanus CCM clusterDepositorInsertSecond CCM operon cloned from H. neapolitanus
UseTagsExpressionBacterialMutationPromoterPLtet0-1 promoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAB061i3
Plasmid#79230PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPpro1 rpoZ; PlacIq lacI
UseTagsExpressionBacterialMutationPromoterPpro1; PlacIqAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET30a-HS-Tn5
Plasmid#127916PurposeProkaryotic expression of Hyperstable Tn5 (HS-Tn5) for Illumina library preperation. Developed for the large scale ChIP-Seq and ATAC-Seq experiments for the fruitENCODE and Maize Cistrome projects.DepositorInsertHyperstable Tn5
UseTagsE coli elongation factor Ts and Mxe intein - Chit…ExpressionBacterialMutationE54K, L372PPromoterT7Available sinceJuly 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
plRL2
Plasmid#163631PurposeOptimized M. tuberculosis CRISPRi plasmid; L5 integrating.DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterTet-OnAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
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Nampt-PET28a
Plasmid#25630DepositorInsertNampt (Nampt Mouse)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 9, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TEV-U1AF37MF77M
Plasmid#168267PurposeExpresses human dmU1A with the F37M/F77M double mutantDepositorInsertU1A RRM1 crystallization module (SNRPA Human)
UseTags6xHis tag N-terminus, TEV protease cleavage siteExpressionBacterialMutationchanged F37M and F77MPromoterT7 promotorAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
+36GFP-LPETG
Plasmid#71754Purpose+36GFP-LPETG for expression in E.ColiDepositorInsert(GGS)9_(pos36GFP)_6XHis_LPETG
UseTags6XHis and Sortase (LPETG)ExpressionBacterialMutationPromoterAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-152
Plasmid#118566PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertsGFP2
UseMinitn7 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only