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Plasmid#177903PurposeBacterial expression of his10-tagged human ICOS Intracellular DomainDepositorInsertICOS (aa 162-199 only) (ICOS Human)
UseTags10xHis-Thrombin cut site-T7 tagExpressionBacterialMutationPromoterT7Available sinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLIC-EcTOP
Plasmid#224463PurposeExpression of recombinant Escherichia coli topoisomerase IDepositorInsertEscherichia coli TopA
UseTags6xHis tagExpressionBacterialMutationPromoterT7Available sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
proinsulin-msGFP2 (C48S C70S)
Plasmid#218956PurposeExpresses proinsulin fused to msGFP2 (C48S C70S) in bacteriaDepositorInsertproinsulin-msGFP2 C48S C70S
UseTagsproinsulinExpressionBacterialMutationPromoterT5Available sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDawn
Plasmid#43796PurposeEmpty backbone for blue-light-regulated expression of a gene of interest. Expression is induced under blue illumination.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
PET-HiFi SpCas9-NLS-6xHis
Plasmid#207376PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertHiFi SpCas9
UseCRISPRTags6xHisExpressionBacterialMutationR691APromoterT7Available sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBA560
Plasmid#186236PurposepTet-eLbuCas13a crRNA entry vectorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMCSG7-Wt-NmeCas9
Plasmid#71474Purposebacterial pMCSG7 expression vector expressing Wildtype Nme cas9 with T7 promoter, N-terminal His tag and TEV siteDepositorInsertpMCSG7-WT-NmeCas9
UseTags6HisExpressionBacterialMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFDH
Plasmid#131706PurposeExpress constitutively an NAD-dependent formate dehydrogenase from Pseudomonas sp. 101 in E. coliDepositorInsertformate dehydrogenase
UseTagshis-tagExpressionBacterialMutationPromoterAvailable sinceNov. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/p-bacteria
Plasmid#64325PurposeExpresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPR; Tetracycline-inducibleTags3xFLAGExpressionBacterialMutationD10A, H840APromoterpLtetO-1Available sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLS.400
Plasmid#187201PurposeExpresses retron Eco4 RTDepositorInsertRetron Eco4 Reverse Transcriptase
UseSynthetic BiologyTagsExpressionBacterialMutationPromotermphRAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-X0
Plasmid#115565PurposeControl vector expressing GFP alone without any consensus repeatsDepositorInsertGreen Fluorescent Protein
UseTagsExpressionBacterial and YeastMutationPromoterADH1Available sinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTrcHIS-wt7D12
Plasmid#125268PurposeExpresses wild type 7D12 anti-EGFR nanobodiesDepositorInsert7D12 nanobody
UseTagsHis-TagExpressionBacterialMutationPromoterTrcAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCE38
Plasmid#174432PurposeC. auris LEUpOUT HYG marker CAS9 expression construct. Use with pCE41 gRNA expression construct.DepositorInsertC. auris LEU2 1 of 2, pENO1, Cas9, HYG 1 of 2
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCE41
Plasmid#174433PurposeC. auris LEUpOUT HYG marker gRNA expression construct. Use with pCE38 CAS9 expression construct.DepositorInsertHYG 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2
UseCRISPRTagsExpressionBacterial and YeastMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAB061i
Plasmid#79226PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPpro1 rpoZ-HA4; PlacIq lacI
UseTagsExpressionBacterialMutationPromoterPpro1; PlacIqAvailable sinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSU-araC-Cas9
Plasmid#120423PurposeMultigene editing in the Escherichia coli genome using the CRISPR-Cas9 systemDepositorInsertCas9
UseCRISPRTagsExpressionBacterialMutationPromoteraraBADAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEvolvR-enCas9-PolI5M
Plasmid#113078PurposeExpresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.DepositorInsertenCas9-PolI5M
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Pro-IL-1α
Plasmid#214341PurposeProtein overexpression in bacteriaDepositorInsertIL1A (IL1A Human)
UseTagsHis-TEV and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET16b-PCNA
Plasmid#134898PurposeBacterial expression and purification of human PCNADepositorInsertPCNA (PCNA Human)
UseTagsuntaggedExpressionBacterialMutationfully synthetic, codon optimized for bacterial ex…PromoterT7Available sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET11phiC31poly(A)
Plasmid#18942DepositorInsertphiC31 integrase (int Streptomyces phage phiC31)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2008AvailabilityAcademic Institutions and Nonprofits only