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Addgene
Showing: 1221 - 1240 of 27067 results

  1. pJK403

    Plasmid
    #72416
    Purpose
    Produces Acetobacter aceti 1023 Est07, N-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    arylesterase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72416-plasmid-map-sequence-id-135537

    Enlarge

  2. pJK404

    Plasmid
    #72417
    Purpose
    Produces Acetobacter aceti 1023 Est12, N-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    acyl-CoA thioesterase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    additional N-terminal 15 residues MSETYGTGQKEQHC,…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72417-plasmid-map-sequence-id-135656

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  3. pJK405

    Plasmid
    #72418
    Purpose
    Produces Acetobacter aceti 1023 Est13, N-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    arylesterase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72418-plasmid-map-sequence-id-135782

    Enlarge

  4. pJK406

    Plasmid
    #72420
    Purpose
    Produces Acetobacter aceti 1023 Est05, C-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    hypothetical protein
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    additional N-terminal MTFLYSICHIVMNIVSSTPHFNVWICF…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72420-plasmid-map-sequence-id-135533

    Enlarge

  5. pJK407

    Plasmid
    #72421
    Purpose
    Produces Acetobacter aceti 1023 Est06, C-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    lipase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    lacks Met1 and Thr2 in annotated (v1) sequence
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72421-plasmid-map-sequence-id-134675

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  6. pJK408

    Plasmid
    #72422
    Purpose
    Produces Acetobacter aceti 1023 Est10, C-terminal His5 tag
    Depositor
    T. J. Kappock
    Insert
    feruloyl esterase
    Use
    Tags
    His5
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72422-plasmid-map-sequence-id-140429

    Enlarge

  7. pJK409

    Plasmid
    #72423
    Purpose
    Produces Acetobacter aceti 1023 Est14, C-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    arylesterase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72423-plasmid-map-sequence-id-135791

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  8. pJK551

    Plasmid
    #72425
    Purpose
    Produces Acetobacter aceti 1023 putative acyl-CoA:carboxylate CoA-transferase, N-terminal His6 tag (H6AaUctD)
    Depositor
    T. J. Kappock
    Insert
    putative CoA-transferase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72425-plasmid-map-sequence-id-133997

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  9. pJK579

    Plasmid
    #72426
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag (AarCH6), additional unique internal restriction enzyme sites
    Depositor
    T. J. Kappock
    Insert
    succinyl-CoA:acetate CoA-transferase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    4 silent mutations introduce unique internal SpeI…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72426-plasmid-map-sequence-id-133996

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  10. pJK267

    Plasmid
    #72432
    Purpose
    Acetobacter aceti 1023 gDNA
    Depositor
    T. J. Kappock
    Insert
    Acetobacter aceti 1023 gDNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    n/a
    Availability
    Academic Institutions and Nonprofits only

  11. pJK268

    Plasmid
    #72436
    Purpose
    Produces Acetobacter aceti 1023 del7-GloA
    Depositor
    T. J. Kappock
    Insert
    lactoylglutathione lyase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Missing residues 1-7
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72436-plasmid-map-sequence-id-132660

    Enlarge

  12. pJK637

    Plasmid
    #72437
    Purpose
    Acetobacter aceti 1023 mutS, partial gene
    Depositor
    T. J. Kappock
    Insert
    DNA mismatch repair protein MutS (partial gene)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    deletes nt 1-767 and nt 2086-2655 of mutS gene
    Promoter
    n/a
    Availability
    Academic Institutions and Nonprofits only
    72437-plasmid-map-sequence-id-132658

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  13. pNC2

    Plasmid
    #72438
    Purpose
    Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1); NCAIR synthetase (EcPurK)
    Depositor
    T. J. Kappock, JoAnne Stubbe
    Article
    Meyer et al Biochemistry. 1999 Mar 9;38(10):3012-8.
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Pro64 to Arg; Asp65 to His; Gln206 to Arg
    Promoter
    T7 and n/a
    Availability
    Academic Institutions and Nonprofits only
    72438-plasmid-map-sequence-id-140433

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  14. pJK523

    Plasmid
    #72442
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes, with N-terminal His6 tag (H6AaOrfX)
    Depositor
    T. J. Kappock
    Insert
    hypothetical protein
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    synthetic gene with ~40 silent substitutions
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72442-plasmid-map-sequence-id-133863

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  15. pJK536

    Plasmid
    #72444
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes (AaOrfX)
    Depositor
    T. J. Kappock
    Insert
    hypothetical protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    synthetic gene with ~40 silent substitutions
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72444-plasmid-map-sequence-id-136729

    Enlarge

  16. pJK538

    Plasmid
    #72445
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein with N-terminal His6 tag (H6AaOrfX); N5-carboxyaminoimidazole ribonucleotide mutase (AaPurE1)
    Depositor
    T. J. Kappock
    Inserts
    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7 and n/a
    Availability
    Academic Institutions and Nonprofits only
    72445-plasmid-map-sequence-id-133868

    Enlarge

  17. pJK540

    Plasmid
    #72446
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein with N-terminal His6 tag (H6AaOrfX); N5-carboxyaminoimidazole ribonucleotide mutase (AaPurE1); NCAIR synthetase (AaPurK)
    Depositor
    T. J. Kappock
    Inserts
    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7 and n/a
    Availability
    Academic Institutions and Nonprofits only
    72446-plasmid-map-sequence-id-133865

    Enlarge

  18. pJK541

    Plasmid
    #72447
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein (AaOrfX); N5-carboxyaminoimidazole ribonucleotide mutase (AaPurE1); NCAIR synthetase (AaPurK)
    Depositor
    T. J. Kappock
    Inserts
    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7 and n/a
    Availability
    Academic Institutions and Nonprofits only
    72447-plasmid-map-sequence-id-133869

    Enlarge

  19. pJK547

    Plasmid
    #72449
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide synthetase, Val319>Phe mutant (AaPurK-V319F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Val319 to Phe
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72449-plasmid-map-sequence-id-133874

    Enlarge

  20. pJK550

    Plasmid
    #72450
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with inserted Val2 (AaPurE1-V2ins)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    inserted Val2
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72450-plasmid-map-sequence-id-133877

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Showing: 1221 - 1240 of 27067 results