Skip to main content
Addgene
Showing: 1201 - 1220 of 28287 results

  3. pET15-MHL

    Plasmid
    #26092
    Purpose
    SGC Empty backbone for bacterial expression under T7 promoter. Uses infusion based cloning method.
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    His and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Available since
    Sept. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
    26092-plasmid-map-sequence-id-12250

    Enlarge

  5. pBbB8k-csg-NbGFP

    Plasmid
    #166858
    Purpose
    Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a GFP-specific nanobody via a flexible linker. Derived from https://www.addgene.org/35363/.
    Depositor
    Neel Joshi
    Article
    Birnbaum et al Adv Sci (Weinh). 2021 May 3;8(11):200469
    Insert
    csgBACEFG, with GFP-specific nanobody fused to CsgA via flexible linker
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    166858-plasmid-map-sequence-id-329027

    Enlarge

  6. pPFO*

    Plasmid
    #120400
    Purpose
    Expresses Perfringolysin O cholesterol sensor, has N-terminal His6-tag and three point mutations, A71T, C459A, and Y181A. Y181A prevents PFO's lytic activity at 4°C.
    Depositor
    Arun Radhakrishnan
    Article
    Das et al Proc Natl Acad Sci U S A. 2013 Jun 25;11
    Insert
    Mutant form of Perfringolysin O
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    Amino acids 1-27 are deleted, starts at amino aci…
    Promoter
    Available since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    120400-plasmid-map-sequence-id-233255

    Enlarge

  12. mAIRN HO

    Plasmid
    #207411
    Purpose
    Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).
    Depositor
    Karlene Cimprich
    Article
    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do
    Insert
    mAIRN
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    207411-plasmid-map-sequence-id-422696

    Enlarge

  13. mAIRN CD

    Plasmid
    #207412
    Purpose
    Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).
    Depositor
    Karlene Cimprich
    Article
    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do
    Insert
    mAIRN
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    207412-plasmid-map-sequence-id-422689

    Enlarge

Showing: 1201 - 1220 of 28287 results