Showing: 101 - 120 of 27067 results
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Plasmid#51768PurposeExpresses PP1 alpha residues 7-330DepositorInsertpp1 alpha
UseTagsHis, TEV protease site, and Thio-6ExpressionBacterialMutationContains PP1 alpha residues 7-330PromoterT7-lacAvailabilityAcademic Institutions and Nonprofits only -
RP1B-PP1beta
Plasmid#51769PurposeExpresses PP1 beta residues 6-327DepositorInsertpp1 beta
UseTagsHis, TEV protease site, and Thio-6ExpressionBacterialMutationContains PP1 beta residues 6-327PromoterT7-lacAvailabilityAcademic Institutions and Nonprofits only -
RP1B-PP1gamma
Plasmid#51770PurposeExpresses PP1 gamma residues 7-323DepositorInsertpp1 gamma
UseTagsHis, TEV protease site, and Thio-6ExpressionBacterialMutationContains PP1 gamma residues 7-323PromoterT7-lacAvailabilityAcademic Institutions and Nonprofits only -
Sirius/pRSETB
Plasmid#51956Purposebacterial expression of Sirius, a pH-insensitive and photostable ultramarine fluorescent proteinDepositorInsertSirius
UseTags6xHisExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Ateam1.03-nD/nA/pRSETB
Plasmid#51960Purposebacterial expression of ATeam1.03-nD/nA, a FRET-based ATP indicatorDepositorInsertATeam1.03-nD/nA
UseTags6xHisExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Nano-lantern/pRSETB
Plasmid#51969Purposebacterial expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imagingDepositorInsertNano-lantern
UseTags6xHisExpressionBacterialMutationS257G in Rluc8PromoterAvailabilityAcademic Institutions and Nonprofits only -
Nano-lantern(Ca2+)_600/pRSETB
Plasmid#51976Purposebacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=600nMDepositorInsertNano-lantern_CaM_E104Q-2G
UseTags6xHisExpressionBacterialMutationS257G in Rluc8PromoterAvailabilityAcademic Institutions and Nonprofits only -
Nano-lantern(Ca2+)_300/pRSETB
Plasmid#51977Purposebacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=300nMDepositorInsertNano-lantern_CaM_E104Q-3GS
UseTags6xHisExpressionBacterialMutationS257G in Rluc8PromoterAvailabilityAcademic Institutions and Nonprofits only -
Nano-lantern(Ca2+)_150/pRSETB
Plasmid#51978Purposebacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=150nMDepositorInsertNano-lantern_CaM-2G
UseTags6xHisExpressionBacterialMutationS257G in Rluc8PromoterAvailabilityAcademic Institutions and Nonprofits only -
Nano-lantern(Ca2+)_117/pRSETB
Plasmid#51979Purposebacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=117nMDepositorInsertNano-lantern_CaM-2GS
UseTags6xHisExpressionBacterialMutationS257G in Rluc8PromoterAvailabilityAcademic Institutions and Nonprofits only -
Nano-lantern(Ca2+)_63/pRSETB
Plasmid#51980Purposebacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=63nMDepositorInsertNano-lantern_CaM_E104Q-4GS
UseTags6xHisExpressionBacterialMutationS257G in Rluc8PromoterAvailabilityAcademic Institutions and Nonprofits only -
Nano-lantern(Ca2+)_11/pRSETB
Plasmid#51981Purposebacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=11nMDepositorInsertNano-lantern_CaM-4GS
UseTags6xHisExpressionBacterialMutationS257G in Rluc8PromoterAvailabilityAcademic Institutions and Nonprofits only -
Nano-lantern(ATP1)/pRSETB
Plasmid#51988Purposebacterial expression of a Nano-lantern-based luminescent ATP indicatorDepositorInsertNano-lantern_ATP1
UseTags6xHisExpressionBacterialMutationS257G in Rluc8PromoterAvailabilityAcademic Institutions and Nonprofits only -
Nano-lantern(ATP0)/pRSETB
Plasmid#51989Purposebacterial expression of a Nano-lantern-based luminescent ATP indicatorDepositorInsertNano-lantern_ATP0
UseTags6xHisExpressionBacterialMutationS257G in Rluc8PromoterAvailabilityAcademic Institutions and Nonprofits only -
CT-Nano-lantern(ATP1)/pRSETB
Plasmid#51990Purposebacterial expression of a Nano-lantern-based luminescent ATP indicator, targeted to chloroplastsDepositorInsertNano-lantern_ATP1
UseTags6xHis and chloroplast transit peptidExpressionBacterialMutationS257G in Rluc8PromoterAvailabilityAcademic Institutions and Nonprofits only -
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pCR4-Dact1(1250-1601)
Plasmid#52046PurposeTo generate mRNA in situ probe against mouse Dact1 (Probe C in PMID 17013874). For antisense cut with Spe1, transcribe with T7. For sense cut with Not1, treat with T4 polymerase, transcribe with T3.DepositorInsertDact1(1250-1601) (Dact1 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCRT7 NT Topo tetR/pLtetO Amp‐WT sfGFP
Plasmid#52053PurposeUsed in phosphoprotein synthesis. Replace the sfGFP with gene of interest (GOI). Add TAG amber codon(s) at the position(s) in GOI where phosphoserine incorporation is desired.DepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromotertetR/pLtetOAvailabilityAcademic Institutions and Nonprofits only -
pKD‐SepRS-EFSep-5x tRNASep (B40 OTS)
Plasmid#52054PurposeUsed in phosphoprotein synthesis. Expresses the Sep-accepting tRNA (tRNASep), the Sep-tRNA synthetase (SepRS) and an engineered EF-Tu (EFSep)DepositorInsertsSepRS
EF-Sep
5x tRNA Sep
UseTagsExpressionBacterialMutationHis67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…PromoterPtacAvailabilityAcademic Institutions and Nonprofits only -
pHnCB
Plasmid#52064Purposeexpresses nine genes from the H. neapolitanus carboxysome operon; IPTG-inducibleDepositorInsertCbbL, CbbS, CSOS2, CSOS3, CSOS4A, CSOS4B, CSOS1C, CSOS1A, CSOS1B
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 101 - 120 of 27067 results