32,838 results
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Plasmid#185784PurposeEncodes enzyme tyrosinase linked to P(11)4 self-assembling peptide via a GS-linker sequenceDepositorInsertTyr-P(11)4
TagsHis-tagExpressionBacterialPromoterT7Available SinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TmCA-P(11)4
Plasmid#185783PurposeEncodes thermostable carbonic anhydrase enzyme linked to P(11)4 self-assembling peptide via a GS-linker sequenceDepositorInsertTmCA-P(11)4
TagsHis-tagExpressionBacterialPromoterT7Available SinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET51b_SNAP-ecDHFR
Plasmid#187108PurposeExpression of SNAP-ecDHFR fusion in bacteriaDepositorInsertSNAP-ecDHFR
TagsHisx10 and StrepExpressionBacterialPromoterT7Available SinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET30-T7-His-sfGFP
Plasmid#180755PurposepET30 vector missing lacO with sfGFP-S-tag inserted between the Bgl II and T7 terminatorDepositorInsertsuperfolder green fluorescent protein
Tagsstrep tagExpressionBacterialAvailable SinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-ChE1 v4.5
Plasmid#185755PurposeBacterial expression of ChE1 v4.5DepositorInsertChE1 v4.5
TagsHis10ExpressionBacterialPromoterT7Available SinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSub-MBP-GFP(2M-IKQE)
Plasmid#185757PurposeBacterial expression of MBP-GFP(2M IKQE)DepositorInsertGFP(2M-IKQE)
TagsMBPExpressionBacterialPromotertacAvailable SinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-His6-Ubc9-K14R
Plasmid#185759PurposeBacterial expression of His6-Ubc9-K14RDepositorInsertSUMO-conjugating enzyme UBC9 (UBE2I Human)
TagsThrombin-cleavable His6-tagExpressionBacterialMutationK14RPromoterT7Available SinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNSSCP
Plasmid#183188PurposePACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; DivIVA-sfGFP expressed under TDepositorInsertsDivIVA and superfolder GFP fusion protein
SpmX450 and T7 181+ fusion protein
T7 RNAP 1-179 variant and SpmX450 fusion protein
mRFP1 and popZ fusion protein
ExpressionBacterialAvailable SinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pfa6a mScarlet-TsCC(B)::KANMX
Plasmid#176397PurposePlasmid for cloning mScarlet fused to TsCC(B) coiled coil into yeast.DepositorInsertmScarlet-TsCC(B)
TagsmScarlet fused to TsCC(B)ExpressionBacterial and YeastPromoternoneAvailable SinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
NSSCP
Plasmid#183190PurposemRFP-PopZ expressed under J23116 promoter; split N terminal of TEV protease-SpmXΔC expressed under Tac promoter; SpmXΔC-split C terminal of TEV protease expressed under Tac promoter; DivIVA-sfGFP-TEVDepositorInsertsmRFP-PopZ
DivIVA-GFP(tevR-50)
SpmX-TEV(C)
TEV(N)-SpmX
ExpressionBacterialAvailable SinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
Paxillin
Plasmid#186142PurposeFor expresseion of recombinant paxillin in bacteriaDepositorInsertPXN (PXN Human)
TagsGSTExpressionBacterialMutationPaxillin C1997A (silent mutation for cloning)PromoterLacAvailable SinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
Nephrin
Plasmid#186134PurposeFor expression of recombinant nephrin in bacteria cellsDepositorInsertNPHS1 (NPHS1 Human)
TagsHis6 and MBPExpressionBacterialMutationAmino acid residues 1174-1223, with mutations: Y1…PromoterLacAvailable SinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD-iGECInano
Plasmid#186189PurposeExpression of small FRET-based near-infrared genetically-encoded calcium indicator in bacteriaDepositorInsertiGECInano
ExpressionBacterialPromoterAraCAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
CD Ugi1
Plasmid#177194PurposeGreengate entry vector pGGC000 modified to contain bladderwort insulator 1DepositorInsertUtricularia gibba insulator 1
ExpressionBacterialAvailable SinceJune 28, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
CD Ugi3
Plasmid#177195PurposeGreengate entry vector pGGC000 modified to contain bladderwort insulator 3DepositorInsertUtricularia gibba insulator 3
ExpressionBacterialAvailable SinceJune 28, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGST2-TSG101 (2-145)
Plasmid#184810PurposeBacterial expression of TSG101 UEV domain (aa 2-145)DepositorAvailable SinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG8H-EWS_1-264
Plasmid#180467PurposeExpresses the construct His8-EWS_1-264 with a TEV cleavage site for the removal of the His8 tagDepositorAvailable SinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIT5_KT
Plasmid#173773PurposeBacterial genomic integration vectorDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCJH065_proD_Cas12c_DualRNA-GFP+HDVrz_CAM
Plasmid#183077PurposePlasmid expressing Cas12c tracrRNA and GFP-targeting crRNA separately, with the crRNA ending with a HDV ribozyme sequence.DepositorInsertCas12c tracrRNA and crRNA ending with a HDV ribozyme
UseCRISPRExpressionBacterialAvailable SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
RBM4_2xRRM-ZNF_pGEX
Plasmid#135154PurposeRecombinant expression of RBM4 with dual-affinity tagDepositorAvailable SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only