30,609 results

1. WT N-His Ana GvpC in pET28a

   Plasmid

   #153294

   Purpose

   Expresses N-terminal His-tagged GvpC from Anabaena flos-aquae in E.coli

   Depositor

   Mikhail Shapiro

   Article

   Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

   Insert

   Gas vesicle protein C (N-terminal his-tagged)
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Added a hexahistidine tag after the methionine at…

   Promoter

   T7

   Available Since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBbE6k-RFP

   Plasmid

   #35288

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   RFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   LlacO-1

   Available Since

   Feb. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. JMJD2C

   Plasmid

   #39070

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   JMJD2C (KDM4C Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pEJS1027-pMCSG7-SmuCas9

   Plasmid

   #121541

   Purpose

   Expresses a 6X-His tagged type II-C Cas9 from S. muelleri in bacterial cells

   Depositor

   Erik Sontheimer

   Article

   Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

   Insert

   SmuCas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJMP1290

   Plasmid

   #119267

   Purpose

   construction intermediate

   Depositor

   Carol Gross, Jason Peters, Oren Rosenberg

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   sgRNA NT1/RR1 (rfp)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Promoter

   Available Since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET15b+PCNA1 P186C

   Plasmid

   #66173

   Purpose

   Expresses the P186C mutant of Sulfolobus solfataricus PCNA1

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

   Insert

   DNA polymerase sliding clamp 1
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   P186C

   Promoter

   T7 promoter

   Available Since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHH0103_SIAH2_Sina

   Plasmid

   #109813

   Purpose

   Protein expression and purification of SIAH2_Sina

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   SIAH2_Sina (SIAH2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX SHP-1(N)-SH2

   Plasmid

   #46495

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   SHP-1(N) (PTPN6 Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 1-109

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJK408

   Plasmid

   #72422

   Purpose

   Produces Acetobacter aceti 1023 Est10, C-terminal His5 tag

   Depositor

   T. J. Kappock

   Insert

   feruloyl esterase
   

   Use

   Tags

   His5

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHisAlix deltaPRD

    Plasmid

    #42577

    Depositor

    James Hurley

    Article

    Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

    Insert

    delta-PRD ALIX (residues 1-702) (PDCD6IP Human)
    

    Use

    Tags

    HIS-TEV

    Expression

    Bacterial

    Mutation

    silent mutation A1617G (numbering in ALIX gene)

    Promoter

    Available Since

    Dec. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pGMEH-P38-luxAB-ssrAec

    Plasmid

    #27062

    Depositor

    Dirk Schnappinger

    Article

    Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

    Insert

    P#38-luxAB-ssrAec
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pC031 - RcrCas13a from Rhodobacter capsulatus R121

    Plasmid

    #91921

    Purpose

    Expresses RcrCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    RcrCas13a and guide expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOP5MP

    Plasmid

    #112612

    Purpose

    Used for expression of protein in E. coli as PreScission cleavable N-terminual 8His and MBP tag fusions and with C-terminal Avi-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8His-MBP and Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCYB-TnsA

    Plasmid

    #17356

    Depositor

    Nancy Craig

    Article

    Hickman et al Mol Cell. 2000 Jun . 5(6):1025-34.

    Insert

    TnsA (tnsA E. coli transposon Tn7)
    

    Use

    Tags

    Intein-Chitin binding domain

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pMS33: pTwinStrep-SUMO-PmcTnsD

    Plasmid

    #168166

    Purpose

    PmcTnsD expression construct for protein purification.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Insert

    PmcCAST TnsD
    

    Use

    Tags

    6X His, Twin Strep and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCFT7.2

    Plasmid

    #208808

    Purpose

    Expresses the fluorescent protein FAST

    Depositor

    François-Xavier Campbell-Valois

    Article

    Tchagang et al Front Microbiol. 2023 Oct 20;14:1245755.

    Insert

    FAST
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Oct. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAEM7

    Plasmid

    #136422

    Purpose

    salL gene from S. tropica CNB-440 ligated into pHIS8

    Depositor

    Bradley Moore

    Article

    Eustaquio et al Nat Chem Biol. 2008 Jan;4(1):69-74. doi:

    Insert

    salL
    

    Use

    Tags

    His8 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pWL502-crRNA

    Plasmid

    #174383

    Purpose

    The plasmids carrying a mini-CRISPR array were used to express crRNAs; article demonstrates use in Haloferax mediterranei

    Depositor

    Jing Han

    Article

    Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

    Insert

    a crRNA targeting the template strand
    

    Use

    CRISPR; Archaeal expression

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PhaR promoter

    Available Since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. p2CT-Cdi

    Plasmid

    #83044

    Purpose

    Expresses Corynebacterium diphtheriae Cas9 (CdiCas9)

    Depositor

    Jennifer Doudna

    Article

    Ma et al Mol Cell. 2015 Nov 5;60(3):398-407. doi:

    Insert

    CdiCas9
    

    Use

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

20. SDC229-B03

    Plasmid

    #58763

    Purpose

    bacterial expression of PDB 4PYZ

    Depositor

    Cheryl Arrowsmith

    Insert

    PDB:4PYZ (USP7 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Bacterial expression for structure determination;…

    Promoter

    T7

    Available Since

    Aug. 5, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits