31,572 results
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Plasmid#40737PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertNCOR2 (NCOR2 Human)
Tags6xHis TEVExpressionBacterialMutationResidues 615 to 685 of the SANT2 domain of Nuclea…Available SinceOct. 18, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRpoH-Wasabi
Plasmid#86107PurposemWasabi reporter gene driven by RpoH promoterDepositorInsertmWasabi
Tags6xHisExpressionBacterialPromoterpRpoHAvailable SinceFeb. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYTK084
Plasmid#65191PurposeEncodes KanR-ColE1 as a Type 8 part to be used in the Dueber YTK systemDepositorInsertKanR-ColE1
ExpressionBacterialAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGWB602-mCherry-sfGFP
Plasmid#213860PurposeFor Agrobacterium transformation. mCherry-sfGFP under the control of the cauliflower mosaic virus (CaMV) 35S promoter.DepositorInsertmCherry-sfGFP
ExpressionBacterial and PlantPromoterCaMV 35SAvailable SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDB026a
Plasmid#109260PurposeAccessory plasmid for PACE of MjTyrRS variantsDepositorInsertPpsp_pIII(P29*); luxAB; PproK_TyrT(Opt, CUA);
ExpressionBacterialMutationamber codon at position 29 gIIIPromoterPpsp; PproKAvailable SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZA-GFP-R5
Plasmid#106256PurposeConstitutively expresses GFP fused with silaffin-R5 silica affinity peptideDepositorInsertGreen fluorescent protein
TagsAdded silaffin R5 peptide to C terminus of GFP af…ExpressionBacterialAvailable SinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAVA-LGF2-Gal11p
Plasmid#127481PurposePositive control for the AVA-seq system. LGF2 (lambda CI associated) and Gal11p (RNAp associated) interactionDepositorInsertLGF2-Gal11p (GAL11 Budding Yeast)
ExpressionBacterialAvailable SinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAVA
Plasmid#127464PurposeAVA-seq plasmid with duel BstXI sitesDepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAVA-LGF2(fs)-Gal11p(fs)
Plasmid#127483PurposeNegative control for the AVA-seq system. LGF2 (with one nucleotide insertion) (lambda CI associated) and Gal11p (with one nucleotide insertion) (RNAp associated) interactionDepositorInsertLGF2 (frame shift)-Gal11p (frame shift) (GAL11 Budding Yeast)
ExpressionBacterialMutationOne nucleotide inserted between lambda CI and LGF…Available SinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAVA-Gal11p-LGF2(fs)
Plasmid#127482PurposeNegative control for the AVA-seq system. Gal11p (lambda CI associated) and LGF2 (with one nucleotide insertion) (RNAp associated) interactionDepositorInsertGal11p-LGF2(frame shifted) (GAL11 Budding Yeast)
ExpressionBacterialMutationLGF2 is frame shifted by the insert of one nucleo…Available SinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBORF-DBD
Plasmid#127463PurposeORF filtering plasmid (lambda cI associated plasmid for pAVA)DepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOP3SU
Plasmid#112599PurposeUsed for expression of protein in E. coli as N-terminal Sumo tag fusionsDepositorTypeEmpty backboneTagsSumoExpressionBacterialAvailable SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
p7XC3GH
Plasmid#47066PurposeFX cloning E.coli expression vector with T7 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tagsDepositorTypeEmpty backboneTags3C protease site (PreScission site), GFP, and dec…ExpressionBacterialPromoterT7Available SinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
4P7A
Plasmid#61954PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMLH1:JMC01L-F09:C205690 (MLH1 Human)
Tags6xHis and MHHHHHHSSGRENLYFQGExpressionBacterialMutationSee commentsPromoterT7Available SinceMarch 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pT7-RiboJ-LacZa-T7
Plasmid#173224PurposepFGC-T7-RJBB to clone genes by Golden Gate (BsaI). To be used in cell-free systemsDepositorTypeEmpty backboneExpressionBacterialPromoterT7Available SinceMarch 9, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
SPR
Plasmid#39161PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertsepiapterin reductase (7,8-dihydrobiopterin:NADP+ oxidoreductase) (SPR Human)
TagsHis TevExpressionBacterialMutationcodon-optimizedAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pL90-Nat-2xHO
Plasmid#139069PurposeCRISPR/Cas9 engineering of the HO endonuclease gene with nourseothricin selectionDepositorInsertTwo guide RNAs targeting the HO endonuclease
ExpressionBacterial and YeastPromoterTDH3Available SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046933
Plasmid#145592PurposeBacterial Expression plasmid for SARS-CoV-2 nsp7DepositorInsertSARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
Endophilin SH3 domains
Plasmid#47401DepositorInsertEndophilin
TagsGSTExpressionBacterialMutationSH3 domain (aa 292-352)PromotertacAvailable SinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
TOPO EFS
Plasmid#68451PurposeTOPO construct expressing EFS for generation of GMAP-compatible promoterDepositorInsertElongation Factor 1α Promoter
UseGmapExpressionBacterialPromoterPlacAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only