29,780 results

1. pASKt15C+SrtA7woH

   Plasmid

   #65020

   Purpose

   Expresses the P94R/E105K/E108A/D160N/D165K/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

   Insert

   sortase A
   

   Use

   Tags

   S tag

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-59, P94R, E105K, E108A, D16…

   Promoter

   tetracycline promoter/operator

   Available since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-Beclin1 (1-242)

   Plasmid

   #24401

   Depositor

   Qing Zhong

   Article

   Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

   Insert

   Beclin1 (BECN1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains amino acids 1-242 only.

   Promoter

   Available since

   April 30, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pET28a(+) 6xHis-TEV-IFIT5

   Plasmid

   #53560

   Purpose

   bacterial expression of IFIT5

   Depositor

   Kathleen Collins

   Article

   Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

   Insert

   IFIT5 (IFIT5 Human)
   

   Use

   Tags

   6xHIS and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pJK265

   Plasmid

   #73598

   Purpose

   Produces Janthinobacterium lividum violacein biosynthesis protein VioC with a N-terminal His6 tag

   Depositor

   T. J. Kappock

   Insert

   violacein biosynthesis protein VioC
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJK266

   Plasmid

   #73326

   Purpose

   Produces Janthinobacterium lividum violacein biosynthesis protein VioD with a N-terminal His6 tag

   Depositor

   T. J. Kappock

   Insert

   violacein biosynthesis protein VioD
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET20b-GpNLuc-6xHis

   Plasmid

   #135932

   Purpose

   Expresses the GpNLuc LumiFluor in bacterial cells.

   Depositor

   Antonio Amelio

   Article

   Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

   Insert

   GpNLuc LumiFluor
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. RhoQ

   Plasmid

   #55695

   Purpose

   expression of mouse RhoQ

   Depositor

   Julian Downward

   Article

   Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

   Insert

   RhoQ (Rhoq Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. DYRK2_HUMAN_D0

   Plasmid

   #79729

   Purpose

   This plasmid encodes the kinase domain of DYRK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   DYRK2 (DYRK2 Human)
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pTKDP-hph

   Plasmid

   #71322

   Purpose

   Donor plasmid - hygromycin resistant donor cassette

   Depositor

   Thomas Kuhlman

   Article

   Tas et al PLoS One. 2015 Sep 2;10(9):e0136963. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a-UVRAG CC

    Plasmid

    #24400

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

    Insert

    UVRAG (UVRAG Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains on the CC domain, amino acids 200-270

    Promoter

    Available since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

11. ATX3-77Q-R388G

    Plasmid

    #184251

    Purpose

    Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track-R388G fused with His-Tag and TEV cleavage sequence (N-terminal)

    Depositor

    Sandra Macedo Ribeiro

    Article

    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111

    Insert

    ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track and point mutation R388G (ATXN3 Synthetic, Human)
    

    Use

    Tags

    6x His-Tag and TEV cleavage site

    Expression

    Bacterial

    Mutation

    C-terminal codon optimization for protein expres…

    Promoter

    T7 promoter

    Available since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. TAF15_RRM-ZNF_pGEX

    Plasmid

    #135111

    Purpose

    Recombinant expression of TAF15 with dual-affinity tag

    Depositor

    Christopher Burge

    Article

    Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

    Insert

    TAF15 (TAF15 Human)
    

    Use

    Tags

    GST-SBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-30a(+)-Syk-tSH2

    Plasmid

    #111269

    Purpose

    express murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form, in E.coli strain Rosetta 2 (DE3)

    Depositor

    Carol Post

    Article

    Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

    Insert

    murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form (Syk Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. EPHA3 (2GSF)

    Plasmid

    #25136

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    epha3.577.947 (EPHA3 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

15. M64 pGEX4T-1-TEV-human beta cat domain N(1-137)

    Plasmid

    #17205

    Depositor

    Randall Moon

    Insert

    beta catenin (CTNNB1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    amino-terminal residues 1-137

    Promoter

    Available since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pGEX4T-1-6AlanineHE1

    Plasmid

    #165098

    Purpose

    bacterial expression of a GST fusion of Human Uba1 with a 6alanine linker between the GST encoded by the plasmid and the inserted Uba1

    Depositor

    Arthur Haas

    Insert

    Uba1 (UBA1 Human)
    

    Use

    Tags

    GST-6-alanine

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. RP1B-PP1beta

    Plasmid

    #51769

    Purpose

    Expresses PP1 beta residues 6-327

    Depositor

    Wolfgang Peti

    Insert

    pp1 beta
    

    Use

    Tags

    His, TEV protease site, and Thio-6

    Expression

    Bacterial

    Mutation

    Contains PP1 beta residues 6-327

    Promoter

    T7-lac

    Available since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pPro29b(+)

    Plasmid

    #25400

    Depositor

    Jay Keasling

    Insert

    
    

    Use

    Synthetic Biology

    Tags

    Unknown

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEX Syk(N)-SH2

    Plasmid

    #46520

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Syk(N) (SYK Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 7-114

    Promoter

    Tac

    Available since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAT773

    Plasmid

    #68941

    Purpose

    NanoLuc expressed constiutively from PAM1 and GH022 RBS, pNBU2 backbone, AmpR

    Depositor

    Timothy Lu, Christopher Voigt

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Insert

    NanoLuc
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge