30,307 results

1. pMP89b HeEst

   Plasmid

   #141206

   Purpose

   Halomonas elongata esterase with TEV cleaving site after the N-terminal Histag.

   Depositor

   Francesca Paradisi

   Article

   Padrosa et al Catalysts 2019, 9(3), 232

   Insert

   HeE
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   Available since

   June 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGEX Supt6h-SH2

   Plasmid

   #46518

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Supt6h (SUPT6H Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 1317-1441

   Promoter

   Tac

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. 1352wUV2 F2bbs kan GCANNNAGGk

   Plasmid

   #18752

   Depositor

   Scot Wolfe

   Article

   Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

   Insert

   3 tandem zinc fingers
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A Kan cassette (1090bp) is inserted between two b…

   Promoter

   Available since

   July 11, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pQE30-AtAEP2

   Plasmid

   #159529

   Purpose

   Expresses Arabidopsis thaliana asparaginyl endopeptidase 2 in E. coli

   Depositor

   Joshua Mylne

   Article

   Bernath-Levin et al Chem Biol. 2015 May 21;22(5):571-82. doi

   Insert

   Arabidopsis thaliana asparaginyl endopeptidase 2
   

   Use

   Tags

   Six-Histidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pQE30-CeAEP1

   Plasmid

   #159528

   Purpose

   Expresses jack bean (Canavalia ensiformis) asparaginyl endopeptidase 1 in E. coli

   Depositor

   Joshua Mylne

   Article

   Bernath-Levin et al Chem Biol. 2015 May 21;22(5):571-82. doi

   Insert

   Canavalia ensiformis asparaginyl endopeptidase 1
   

   Use

   Tags

   Six-Histidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBEST-p70a-UTR3-deGFP-6xHis-T500

   Plasmid

   #92224

   Purpose

   Expression of deGFP using the p70a promoter and UTR3

   Depositor

   Lital Alfonta

   Article

   Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

   Insert

   deGFP
   

   Use

   Synthetic Biology; Cell free protein synthesis (c…

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   p70a

   Available since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pECJ3D

   Plasmid

   #75467

   Purpose

   Toggle switch plasmid containing lacI-pdt3D

   Depositor

   James Collins

   Article

   Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

   Insert

   lacI-pdt3D
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLtetO

   Available since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGEX/PD-L1 3SA

   Plasmid

   #121467

   Purpose

   Expression of GST-human PD-L1 in E. Coli

   Depositor

   Mien-Chie Hung

   Article

   Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

   Insert

   CD274 (CD274 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   S176A, T180A, S184A

   Promoter

   Tac

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET His6 Gamma crystallin TEV LIC cloning vector (2X-T)

   Plasmid

   #29714

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   His6-Gamma crystallin-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDEST-APIC-Cas9

    Plasmid

    #121657

    Purpose

    gateway cloning destination vector for expressing enhancer driven Cas9

    Depositor

    Chun Han

    Article

    Poe et al Genetics. 2018 Nov 30. pii: genetics.118

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLC13

    Plasmid

    #78494

    Purpose

    Expression of Mu-gam under the pBAD promoter

    Depositor

    David Bikard

    Article

    Cui et al Nucleic Acids Res. 2016 Apr 8. pii: gkw2

    Insert

    Mu-gam (Mup10 )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Twitch-3 pRSETB

    Plasmid

    #48204

    Purpose

    Fluorescent reporter for calcium signaling

    Depositor

    Oliver Griesbeck

    Article

    Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

    Insert

    Twitch-3A
    

    Use

    Tags

    6xHIS, cpVenusCD, and mCerulean3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pET28a Gx-NL3

    Plasmid

    #169108

    Purpose

    Expresses protein G fused to 3 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

    Depositor

    Maarten Merkx

    Article

    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

    Insert

    protein G - NanoLuc - NanoLuc - NanoLuc
    

    Use

    Tags

    his-tag and strep-tag

    Expression

    Bacterial

    Mutation

    Ala 24 stop

    Promoter

    T7

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28-mHP1alpha

    Plasmid

    #181911

    Purpose

    Bacterial protein expression vector for HP1alpha

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    mHP1alpha (Cbx5 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET26b_Nb.AD102

    Plasmid

    #131403

    Purpose

    E. coli periplasmic expression of conformational nanobody Nb.AD102 recognizing purified active-state A2a adenosine receptor

    Depositor

    Andrew Kruse

    Article

    McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

    Insert

    Nanobody AD102
    

    Use

    Tags

    6xHis-tag and pelB leader

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAND_Reporter

    Plasmid

    #60591

    Purpose

    AND gate reporter

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    AND_Reporter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBS Wnt-5aHA

    Plasmid

    #17376

    Depositor

    Jan Kitajewski

    Article

    Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

    Insert

    Wnt-5aHA (Wnt5a Mouse)
    

    Use

    Tags

    HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 7, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pAB675

    Plasmid

    #176578

    Purpose

    Expressing three of the five genes of the n-butanol pathway.

    Depositor

    Arpita Bose

    Article

    Bai et al Commun Biol. 2021 Nov 3;4(1):1257. doi:

    Insert

    phaJ, ter, adhE2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUC13

    Plasmid

    #50003

    Purpose

    pUC cloning vector

    Depositor

    Joachim Messing

    Article

    Norrander et al Gene. 1983 Dec;26(1):101-6.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. DNMT1

    Plasmid

    #25165

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    dnmt1.0351.0600 (DNMT1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits