30,880 results

1. pSumo-His6- Sumo-AtTPL(1-209)

   Plasmid

   #177856

   Purpose

   Bacterial Expression of TPL

   Depositor

   Karsten Melcher

   Article

   Ke et al Sci Adv. 2015 Jul 24;1(6):e1500107. doi:

   Insert

   TPL (TPL Mustard Weed)
   

   Tags

   6xHis-SUMO

   Expression

   Bacterial

   Available Since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBTB-2

   Plasmid

   #22818

   Depositor

   Ryan Gill

   Article

   Lynch et al Biotechnol Bioeng. 2006 May 5. 94(1):151

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   March 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJKR-L-ttgR

   Plasmid

   #62566

   Purpose

   Flavonoid biosensor. Low copy plasmid that expresses GFP proportional to flavonoid concentration.

   Depositor

   George Church

   Article

   Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

   Insert

   GFP
   

   Expression

   Bacterial

   Available Since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTac-C-luci

   Plasmid

   #65935

   Purpose

   Theophylline Riboswitch C with luciferase gene

   Depositor

   Justin Gallivan

   Article

   Mishler et al Nucleic Acids Res. 2014 Jun;42(10):6753-

   Insert

   luciferase
   

   Expression

   Bacterial

   Available Since

   Sept. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

5. Endophilin II Full

   Plasmid

   #47409

   Depositor

   Peter McPherson

   Insert

   Endophilin II
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

6. pGEX-6P1-Ataxin3-1-182

   Plasmid

   #22115

   Depositor

   Henry Paulson

   Article

   Winborn et al J Biol Chem. 2008 Sep 26. 283(39):26436-

   Insert

   Ataxin 3 (ATXN3 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 183-373 deleted

   Available Since

   Sept. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

7. hsp70:Stathmin1-mKate2

   Plasmid

   #105969

   Purpose

   heat shock inducible transgenesis, stathmin stmn1 orig. from addgene #31326

   Depositor

   Caren Norden

   Article

   Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

   Insert

   Stathmin1 (STMN1 Human)
   

   Tags

   mKate2

   Expression

   Bacterial

   Available Since

   Feb. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGBW-m4046901

   Plasmid

   #145725

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 ORF7a protein

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 ORF7a protein (ORF7a Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pGEX6P1-DEST-HA

   Plasmid

   #119751

   Purpose

   Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal HA tag.

   Depositor

   Andrew Jackson, Martin Reijns

   Type

   Empty backbone

   Tags

   GST and HA

   Expression

   Bacterial

   Promoter

   tac promoter

   Available Since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-2HMG-eGFP

    Plasmid

    #112737

    Purpose

    Express 2HMG-eGFP in E. coli

    Depositor

    Kyubong Jo

    Article

    Lee et al RSC Adv, 2016, 6, 46291-46298

    Insert

    2HMG-eGFP
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28b-pvdA WT

    Plasmid

    #87098

    Purpose

    expresses PvdA, the ornithine hydroxylase from Pseudomonas aeruginosa

    Depositor

    Audrey Lamb

    Article

    Olucha et al J Biol Chem. 2011 Sep 9;286(36):31789-98

    Insert

    pvdA WT
    

    Tags

    his-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. FXR1

    Plasmid

    #25341

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    FXR1_25 (FXR1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 17, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pAV114

    Plasmid

    #63181

    Purpose

    pRS414 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBAD-RDSmCherry1

    Plasmid

    #89987

    Purpose

    Red-Shifted variants of red fluorescent protein mCherry

    Depositor

    Robert Campbell

    Article

    Shen et al PLoS One. 2017 Feb 27;12(2):e0171257. do

    Insert

    RDSmCherry1
    

    Tags

    6xHis-T7-Xpress

    Expression

    Bacterial

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-22HT-MSI2(8-193)

    Plasmid

    #60356

    Purpose

    Express the RBD of human MSI2 in bacterial cells for protein purification

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    human MSI2 (8-193) (MSI2 Human)
    

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    contains amino acids 8-193 of MSI2

    Promoter

    T7

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. p2CT-His-MBP-Rca_Cas13a_WT

    Plasmid

    #91870

    Purpose

    Bacterial expression for Cas13a

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

    Insert

    RcaCas13a (RcaC2c2) WT
    

    Tags

    His6-MBP-TEV site

    Expression

    Bacterial

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. Brg1 Del1 pET28a

    Plasmid

    #122115

    Purpose

    Expression Brg1 amino acids 1-381 in bacteria

    Depositor

    Paul Herring

    Article

    Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

    Insert

    Brg1 (SMARCA4 Human)
    

    Tags

    His-T7

    Expression

    Bacterial

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDIV489

    Plasmid

    #177697

    Purpose

    Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.

    Depositor

    Uffe Mortensen

    Article

    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

    Inserts

    Cas9
    Nat
    

    Use

    CRISPR

    Tags

    SV40

    Expression

    Bacterial and Yeast

    Promoter

    RNR2p (Debaryomyces hansenii ) and TDH3p (Candida…

    Available Since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. c7_HLL

    Plasmid

    #63125

    Purpose

    cysteine free Mm-cpn mutant

    Depositor

    Tanja Kortemme

    Article

    Hoersch et al Nat Nanotechnol. 2013 Dec;8(12):928-32.

    Insert

    Mm-cpn c7_HLL (NEWENTRY )
    

    Tags

    6x HIS-tag

    Expression

    Bacterial

    Mutation

    C140S-C237S-C286A-C359T-C393A-C470S-C484S

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCoofy35

    Plasmid

    #43985

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Tags

    MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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