33,434 results
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Plasmid#189935PurposeMutagenesis Plasmid: pTet, danQ926, dam, seqA, emrR, ugi and cda1, tetR, CloDF13, KanRDepositorInsertdanQ926, dam, seqA, emrR, ugi and cda1
ExpressionBacterialPromoterpTetAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBAD-6xHis-BglII
Plasmid#196654PurposeDerived from pGLO (BioRad) by the insertion of a 6xHis Tag and a BglII siteDepositorTypeEmpty backboneTags6xHis tagExpressionBacterialAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBR322-Pdp1_NTD-ZFN_L-HiBiT
Plasmid#198319PurposeLoads HiBiT-tagged L fragment of a zinc finger nuclease specific for human AAVS1 locus using Pdp1 packaging domainDepositorInsertPdp1_NTD-ZFN_L-HiBiT
ExpressionBacterialAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE80l B-CN-B
Plasmid#196477PurposeProtein expression of SpyCatcher-ELP-CarHCN-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher, and GRGD site linked with (GGGGS)2 before CarHCNDepositorInsertB-CN-B
ExpressionBacterialPromoterT7Available sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGMC00018 (aka pRC0082)
Plasmid#195320PurposeMinimal plasmid backbone with multiple cloning site for HDR donor DNA constructionDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionBacterial and MammalianAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pnYC-NpM-HsNot7_AF
Plasmid#148741PurposeBacterial Expression of HsNot7DepositorInsertHsNot7 (CNOT7 Human)
ExpressionBacterialAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpH-HsNot9_S
Plasmid#147494PurposeBacterial Expression of HsNot9DepositorInsertHsNot9 (CNOT9 Human)
ExpressionBacterialAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIYN3
Plasmid#197099PurposeTo express the variant of M. jannaschii TyrRS specific for halogenated tyrosines and Nap3 amber suppressor tRNA and allow UAG codon to be translated into these derivatives.DepositorInsertTyrRS variant, Nap3 tRNA
ExpressionBacterialMutationAla70, Thr158, Ser159, Arg286 (TyrRS)Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HisD-miRFP713-HO1
Plasmid#197255PurposeExpresses the protein of miRFP713 and HO1 in bacteriaDepositorInsertmiRFP713
ExpressionBacterialAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HisD-emiRFP670-HO1
Plasmid#197252PurposeExpresses the protein of emiRFP670 and HO1 in bacteriaDepositorInsertemiRFP670
ExpressionBacterialAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE80l ACA
Plasmid#196471PurposeProtein expression of SpyTag-ELP-CarHC-ELP-SpyTagDepositorInsertACA
ExpressionBacterialPromoterT7Available sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOPO717
Plasmid#195302PurposepBAD322-MycaIDCas-lacZsp5, arapBAD promoter expressing type I-D Cascade and one spacer array of McCAST (Tn7575), the single spacer is lacZ spacer 5.DepositorInsertMcCAST Cascade and lacZ spacer 5 in native array
ExpressionBacterialPromoterArabinose inducible arapBADAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
p5059
Plasmid#198154Purposebacterial expression of steC::HaloTag fusionDepositorInsertPsteC steC::L16::HaloTag::HA
TagsHaloTag-HAExpressionBacterialAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJBL3907
Plasmid#193140PurposeClostridium beijerinckii pfl ZTP riboswitch with sfGFP reporterDepositorInsertClostridium beijerinckii (Cbe) pfl ZTP riboswitch with sfGFP
UseSynthetic BiologyExpressionBacterialPromoterJ23119Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJBL6392
Plasmid#193141PurposeSynthetic OFF-logic Clostridium beijerinckii pfl ZTP riboswitch with sfGFP reporterDepositorInsertSynthetic flipped logic Cbe riboswitch with sfGFP
UseSynthetic BiologyExpressionBacterialPromoterJ23119Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJBL6090
Plasmid#193142PurposeClostridium beijerinckii pfl ZTP riboswitch A26∆ OFF control with sfGFP reporterDepositorInsertCbe ZTP Riboswitch sfGFP
UseSynthetic BiologyExpressionBacterialMutationDeleted A26 to disable ligand-bindingPromoterJ23119Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
enz.105-MelA
Plasmid#154049PurposeExpresses MelA in E. coliDepositorPublicationInsertTyrosinase MelA
TagsHis, MBPExpressionBacterialPromoterT7Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
ptRNAfMet-A37
Plasmid#196657PurposePlasmid for overexpression of native initiator tRNA (A37)DepositorInserttRNAfMet-A37 expression cassette
ExpressionBacterialPromotermetY native promoterAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
P74-26 Tail Tube Protein (gp93) in pSMT3
Plasmid#191261PurposeExpresses P74-26 gp93 with a His-SUMO tag in bacterial cellsDepositorInsertP74-26 gp93
TagsHis-SUMOExpressionBacterialPromoterT7Available sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKB237
Plasmid#170030PurposeEncodes LacI-controlled expression of surface-displayed CCL19 and PvirK-mNeonGreen reporterDepositorPublicationInsertsUseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only