32,218 results

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Twitch-2C pcDNA3

Plasmid

#104501

Purpose

fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-2C

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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Pet15b-Internalization Control

Plasmid

#186553

Purpose

Negative Control mediating endosomal localization

Depositor

Jean Gariepy

Article

Mohammed et al J Control Release. 2012 Nov 28;164(1):58

Insert

10R-8His-EGFP

Tags

8xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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NRDP1

Plasmid

#25620

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

NRDP1 (RNF41 Human)

Tags

His

Expression

Bacterial

Mutation

Contains USP8 interaction Domain

Available Since

Aug. 31, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pET28-p53 (1-90) (72P)

Plasmid

#62080

Purpose

Expression of human p53 (1-90) (72P) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

contains TAD/PP residues 1-90; 72P

Promoter

T7

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
pGEX-Nedd4-N / 2524 JM16

Plasmid

#13312

Depositor

Allan Weissman

Article

Hatakeyama et al J Biol Chem. 1997 Jun 13. 272(24):15085-

Insert

Nedd4 (Nedd4 Mouse)

Tags

GST

Expression

Bacterial

Mutation

aa1-52 and aa422-777 deleted

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pmMaple2-CAM

Plasmid

#101149

Purpose

Fluorescent protein for superresolution imaging

Depositor

Xiaowei Zhuang

Article

Wang et al Proc Natl Acad Sci U S A. 2014 Jun 10;11

Insert

E. coli codon optimized mMaple2

Expression

Bacterial

Promoter

pBAD

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pUA66 PcspA GFP Linker

Plasmid

#105604

Purpose

Expresses GFP under temperature sensitive promoter PcspA

Depositor

Pamela Silver

Article

Stirling et al Mol Cell. 2017 Nov 16;68(4):686-697.e3.

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PcspA

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pB1H2w2-12-C81S

Plasmid

#18042

Depositor

Scot Wolfe

Article

Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

Insert

HhaI C81S mutant

Expression

Bacterial

Mutation

Simply used as an insert to drop out between Kpn1…

Available Since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

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pMH-02

Plasmid

#158432

Purpose

pBAD33 vector encoding MotA, eGFP-MotB

Depositor

Francesco Pedaci

Article

Heo et al Sci Rep. 2017 Oct 3;7(1):12583. doi: 10.

Inserts

MotA
MotB

Tags

eGFP

Expression

Bacterial

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pMH-macroHC

Plasmid

#82337

Purpose

Provides a C-terminal macro domain and 6xHis tag for expression in E. coli

Depositor

Michael Hothorn

Article

Wild et al Protein Sci. 2016 Oct 24. doi: 10.1002/p

Insert

SPX domain of the Vacuolar Transporter Chaperone 4 (VTC4 Budding Yeast)

Tags

6xHis non-cleavable and macro domain of human his…

Expression

Bacterial

Mutation

Truncated protein, amino acids 1-178

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pSR284

Plasmid

#125093

Purpose

Expression of ompR(1-122aa)-manR(120-234aa) chimera under PLtetO-1, output Promoter PmntCAB143

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

ompR(REC)-manR(DBD)122
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pHH0103_PACSIN2-1/1

Plasmid

#91330

Purpose

Protein expression and purification of human SH3 domain construct PACSIN2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

PACSIN2-1/1 (PACSIN2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pFAB3833

Plasmid

#47839

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 8 + BCD1 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 8 and BCD1

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pWT037a

Plasmid

#107896

Purpose

W2.1 plasmid. Contains PTetO-SD8-BE2, PLacO-sgRNA1 and is part of CAMERA 2.1

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-SD8-BE2, PLacO-sgRNA1

Expression

Bacterial

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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ISG15 C-term. Domain intein/chitin binding domain (79-156)

Plasmid

#110760

Purpose

Bacterial expression of ISG15 C-term.

Depositor

David Komander

Article

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

ISG15 (ISG15 Human)

Tags

intein/chitin binding domain

Expression

Bacterial

Promoter

T7

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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GST-BRD (1079-1198)

Plasmid

#99507

Purpose

Bacterial expression of mouse CBP bromodomain

Depositor

Peter Wright

Article

Park et al FEBS Lett. 2013 Aug 19;587(16):2506-11.

Insert

mouse CBP BRD (1079-1198) (Crebbp Mouse)

Tags

GST

Expression

Bacterial

Available Since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pSCBe

Plasmid

#110065

Purpose

Trc1O promoter express gene in Synechocystis 6803

Depositor

Devaki Bhaya

Type

Empty backbone

Tags

3' end His tag and 5' end FLAG tag

Expression

Bacterial

Promoter

Trc1O

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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L3MBTL2

Plasmid

#25244

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

L3MBTL2_14 (L3MBTL2 Human)

Tags

His

Expression

Bacterial

Available Since

Aug. 13, 2010

Availability

Industry, Academic Institutions, and Nonprofits
NELFE-mOrange pHis-//

Plasmid

#53303

Purpose

Expresses His-tagged NELF-E-mOrange fusion protein.

Depositor

John Lis

Article

Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

Insert

NELF-E protein (Nelf-E Fly)

Tags

His and mOrange

Expression

Bacterial

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pEM7066

Plasmid

#214004

Purpose

PrpsM driven expression of ssrA-tagged mScarlet-I (LVA)

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

mScarlet-I-LVA

Expression

Bacterial

Promoter

PrpsM

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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