33,539 results
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Plasmid#200950PurposeMBP-tagged E. coli protein expression destination vector for Golden Gate cloningDepositorTypeEmpty backboneTagsMBPExpressionBacterialPromoterT7Available sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only
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pBBR1_DVK_AE(J23100-B0034m-E0040m-B0015)
Plasmid#197659PurposeExpresses GFP under the control of J23100 and B0034mDepositorInsertGFP
UseSynthetic BiologyExpressionBacterialAvailable sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+) ChemoG1
Plasmid#193799PurposeProtein production of ChemoG1 in bacteriaDepositorInsertChemoG1
Tags10xHisExpressionBacterialPromoterT7Available sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+) ChemoG2
Plasmid#193800PurposeProtein production of ChemoG2 in bacteriaDepositorInsertChemoG2
Tags10xHisExpressionBacterialMutationEGFP: A206KPromoterT7Available sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+) ChemoG3
Plasmid#193801PurposeProtein production of ChemoG3 in bacteriaDepositorInsertChemoG3
Tags10xHisExpressionBacterialMutationEGFP:A206K; HT7: L271EPromoterT7Available sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+) ChemoG 3.2
Plasmid#193803PurposeProtein production of ChemoG3.2 in bacteriaDepositorInsertChemoG 3.2
Tags10xHisExpressionBacterialMutationEGFP:A206K, T225R; HT7: L271EPromoterT7Available sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+) ChemoG4
Plasmid#193804PurposeProtein production of ChemoG4 in bacteriaDepositorInsertChemoG4
Tags10xHisExpressionBacterialMutationEGFP:A206K; HT7: E143R, E147R, L271EPromoterT7Available sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
ALIX(P801G)-Strep
Plasmid#180024PurposeCodon-optimized bacterial expression plasmid. Expresses ALIX(1-868, P801G)-Strep. P801G mutation facilitates expression in E. coli.DepositorInsertALIX(1-868, P801G)-Strep (PDCD6IP Human)
TagsStrep tag (WSHPQFEK)ExpressionBacterialMutationP801GAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-ALIX(P801G)
Plasmid#180025PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(1-868, P801G). P801G mutation facilitates expression in E. coli.DepositorInsertGB1-His6-TEV-ALIX(1-868, P801G) (PDCD6IP Human)
TagsGB1-His6-TEVExpressionBacterialMutationP801GAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
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hPU.1ΔN165-pET28b
Plasmid#199796PurposeExpressed human PU.1/Spi-1 ETS domain (residues 165 to 270) in E. coliDepositorAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
SUMO_MT3
Plasmid#200303PurposeBacterial expression plasmids for production of recombinant metallothionein 3 containing N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubilityDepositorAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
H6SUMO-IntC-P53(304-393)
Plasmid#200316Purposebacterial expression of P53 TET-CTD for segmental labelingDepositorInsertp53 (304-393) (TP53 Human)
TagsHis6-SUMO and designed intein CExpressionBacterialPromoterT7Available sinceMay 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOPO685
Plasmid#195300PurposepBAD322-MycaIDCas-PaqCI, arapBAD promoter expressing type I-D Cascade and one spacer array of McCAST (Tn7575), the single spacer is replaced with PaqCI entry site.DepositorInsertMcCAST Cascade and native array with PaqCI entry site
ExpressionBacterialPromoterArabinose inducible arapBADAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hHBEGF
Plasmid#199234PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertProheparin-binding EGF-like growth factor (HBEGF Human)
TagsTrxA-6xHis-S-tagExpressionBacterialPromoterT7Available sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
tracrRNA(scr)-CJ8421_04975 mRNA
Plasmid#199718PurposeExpresses the Campylobacter mRNA CJ8421_04975 and tracrRNA with scrambled anti-repeat sequencesequenceDepositorInsertCJ8421_04975 mRNA and scrambled tracrRNA
UseCRISPRExpressionBacterialAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-GST-Thrombin-TEV-EGFP-hNEMO
Plasmid#199781PurposePlasmid for the expression of GFP labelled NEMO (SMC Internal No. 1977).DepositorAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSET.HaloTag.v7.CoOp.Cys-less
Plasmid#195487PurposeBacterial expression plasmid with HaloTag v7 insert; modified to replace cysteines C61, C262, and optimized to human codon bias/preference for use in oxidizing environments.DepositorInsertHaloTag.v7
Tags6xHISExpressionBacterialMutationC61T, C262VPromoterT7Available sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-sfGFP[D134TAG+N150TAA]
Plasmid#164582Purposesuperfolder GFP with D134TAG and N150TAA mutationsDepositorInsertsfGFP-134TAG+150TAA
Tags6xHisExpressionBacterialMutationD134TAG + N150TAA MutationsPromoterT7Available sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-sfGFP[N150TAA]
Plasmid#164581Purposesuperfolder GFP with N150TAA mutationDepositorInsertsfGFP-150TAA
Tags6xHisExpressionBacterialMutationN150TAA MutationPromoterT7Available sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only