32,167 results
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Plasmid#212123PurposeFor expression of the bacterial gasdermin (bGSDM) encoded by Runella zeae. Protein is expressed with an N-terminal SUMO2 tag.DepositorInsertRunella bGSDM
Tags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available SinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Vitiosangium-bGSDM
Plasmid#212127PurposeFor expression of the bacterial gasdermin (bGSDM) encoded by a Vitiosangium species. Protein is expressed with an N-terminal SUMO2 tag.DepositorInsertVitiosangium bGSDM
Tags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available SinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Gillisia-bGSDM
Plasmid#212128PurposeFor expression of the bacterial gasdermin (bGSDM) encoded by a Gillisia species. Protein is expressed with an N-terminal SUMO2 tag.DepositorInsertGillisia bGSDM
Tags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available SinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Bradyrhizobium-bGSDM
Plasmid#212126PurposeFor expression of the bacterial gasdermin (bGSDM) encoded by a Bradyrhizobium species. Protein is expressed with an N-terminal SUMO2 tag.DepositorInsertBradyrhizobium bGSDM
Tags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available SinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET47b-T7-dAsCas12f
Plasmid#204641PurposeExpression of an inactive AsCas12f in E. coliDepositorInsertinactive AsCas12f
Tags6xHisExpressionBacterialMutationD225APromoterT7Available SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-RBP-GFP(S45C)-ZdK1
Plasmid#185791PurposeEncodes green flourescent protein linked to RNA binding peptide (on N terminal end ) and Z domain fragment (variant 1) of Staphylococcal protein A (on C-terminal) via GS-linkerDepositorInsertRBP-sfGFP(S45C)-ZdK1
Tags10xHis, TEV protease cleavage site after His TagExpressionBacterialMutationSer to Cys mutation at start of GFP, residue 45 i…PromoterT7Available SinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GNA14_G-alpha
Plasmid#109914PurposeProtein expression and purification of GNA14_G-alphaDepositorInsertGNA14_G-alpha (GNA14 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJMP1217
Plasmid#119258Purposerfp "test" strainDepositorInsertsgRNA NT1/RR1 (rfp)
ExpressionBacterialMutationD10A and H840AAvailable SinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T SHP1 CS
Plasmid#8595DepositorAvailable SinceJune 21, 2005AvailabilityAcademic Institutions and Nonprofits only -
UBQLN2_450-624W
Plasmid#176852PurposeExpresses human UBQLN2 construct 450-624 with added C-terminal W in bacterial cellsDepositorInsertUBQLN2_450C_W (UBQLN2 Human)
ExpressionBacterialMutationdeletion of residues 1-449 and addition of C-term…PromoterT7Available SinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_CRK-1/2
Plasmid#91367PurposeProtein expression and purification of human SH3 domain construct CRK-1/2DepositorInsertCRK-1/2 (CRK Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAJT430
Plasmid#202291PurposeRPU integration plasmid (LPattB7)DepositorInsertJ23101-RiboJ00-B0034-YFP
ExpressionBacterialAvailable SinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-dSpCas9-HF1-VP64-6xHis
Plasmid#92118PurposeExpression of dead/inactive increased fidelity SpCas9-HF1-VP64-6xHis in bacterial cellsDepositorInsertdead/inactive SpCas9-HF1-NLS-3xFLAG-VP64
UseCRISPRTags3xFLAG, 6xHis, NLS, and VP64ExpressionBacterialMutationD10A, N497A, R661A, Q695A, H840A, Q926APromoterT7Available SinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGST TCF4 DBD
Plasmid#16488DepositorAvailable SinceApril 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
SMYD3
Plasmid#25318PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 17, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJMP1069
Plasmid#119244Purposerfp "test" strainDepositorInsertsgRNA NT1/RR1 (rfp)
ExpressionBacterialMutationD10A and H840AAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSEVA221TrxA-AID-T7RNAP
Plasmid#167976PurposeExpresses fusion protein TrxA-AID-T7RNAP in E. coliDepositorInsertTrxA-AID-T7RNAP
TagsT7RNAP and TrxAExpressionBacterialPromoterPtetAvailable SinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTCF3 H-2D(d)
Plasmid#180452PurposeE. coli expression of soluble MHC proteinDepositorInsertH-2D(d)
TagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…Available SinceFeb. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_E299A
Plasmid#91855PurposeBacterial expression for Cas13aDepositorInsertLbuCas13a (LbuC2c2) E299A
TagsHis6-MBP-TEV siteExpressionBacterialMutationE299AAvailable SinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21c-His-Xa-Gluc-Xa-C9D-TGinsert
Plasmid#124660Purposereporter protein which emits bright blue fluorescenceDepositorInsertGaussia luciferase-C9D-TGinsert
TagsIEGRTMGDDDGDDDGDDD(FactorXa,TMG,C9D-Tag) and MHHH…ExpressionBacterialMutationchanged glutamic acid 100 to alanine, and glycine…PromoterT7 promoterAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only