30,744 results

1. pTHSSe_37

   Plasmid

   #109237

   Purpose

   PTac driving GFP

   Depositor

   Christopher Voigt

   Article

   Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   sfGFP
   

   Expression

   Bacterial

   Promoter

   PTac

   Available Since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. FXR1

   Plasmid

   #25341

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   FXR1_25 (FXR1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Aug. 17, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

3. pOPS0253

   Plasmid

   #115505

   Purpose

   Suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing gusA.

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Insert

   Rlv3841pNifH pOGG082, gusA pOGG083, T-pharma pOGG003 assembled in pOGG026
   

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. p2CT-His-MBP-Rca_Cas13a_WT

   Plasmid

   #91870

   Purpose

   Bacterial expression for Cas13a

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

   Insert

   RcaCas13a (RcaC2c2) WT
   

   Tags

   His6-MBP-TEV site

   Expression

   Bacterial

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAG8Ha-SERBP1(149-400)

   Plasmid

   #176516

   Purpose

   Expresses the human C-terminal SERBP1 149-400 construct.

   Depositor

   David Libich

   Article

   Baudin et al Front Mol Biosci. 2021 Sep 24;8:744707.

   Insert

   SERBP1 149-400 (SERBP1 Human)
   

   Tags

   His8 tag, linker, TEV site

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-148 and 401-408

   Promoter

   T7

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRC01

   Plasmid

   #114680

   Purpose

   sfGFP.Strands 1-9

   Depositor

   Matthew Good

   Article

   Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

   Insert

   sfGFP Strands 1-9
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCoofy35

   Plasmid

   #43985

   Depositor

   Sabine Suppmann

   Article

   Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

   Type

   Empty backbone

   Tags

   MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPP378

   Plasmid

   #158797

   Purpose

   pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-1(D371A) in MCS1 for expression in E. coli and protein purification

   Depositor

   Jennifer Doudna

   Article

   Pausch et al Science. 2020 Jul 17;369(6501):333-337.

   Insert

   dCasPhi-1 (D371A)
   

   Expression

   Bacterial

   Available Since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEJS1026-pMCSG7-HpaCas9

   Plasmid

   #121540

   Purpose

   Expresses a 6X-His tagged type II-C Cas9 from H. parainfluenzae in bacterial cells

   Depositor

   Erik Sontheimer

   Article

   Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

   Insert

   HpaCas9
   

   Expression

   Bacterial

   Available Since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHH0103_KPNB1_HEAT

    Plasmid

    #110017

    Purpose

    Protein expression and purification of KPNB1_HEAT

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    KPNB1_HEAT (KPNB1 Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. TTR C10S S85C K80D

    Plasmid

    #184743

    Purpose

    bacterial expression of human TTR mutants

    Depositor

    Peter Wright

    Article

    Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

    Insert

    TTR (TTR Human)
    

    Expression

    Bacterial

    Mutation

    C10S, S85C, K80D

    Promoter

    T7

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEM-T ZfHoxc11a

    Plasmid

    #27542

    Depositor

    Victoria Prince

    Insert

    Hoxc11a (hoxc11a Zebrafish)
    

    Expression

    Bacterial

    Available Since

    Feb. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pR6Kcat-rdxA-rpsL

    Plasmid

    #45471

    Depositor

    Michele Swanson

    Article

    Bryan et al Mol Microbiol. 2011 Apr;80(1):231-47. do

    Insert

    cat-rdxA-rpsL
    

    Expression

    Bacterial

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. FDPS

    Plasmid

    #39131

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    FDPS (FDPS Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

15. 4RFQ

    Plasmid

    #64656

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    METTL18:PBC004-E03:C231318 (METTL18 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available Since

    May 15, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

16. ZP16 Wnt-1

    Plasmid

    #16925

    Depositor

    Randall Moon

    Insert

    wnt-1 (wnt1 Zebrafish)
    

    Expression

    Bacterial

    Available Since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

17. L-YARS2-9

    Plasmid

    #166533

    Purpose

    scFv of a human scaffold targeting Tyrosyl-tRNA synthetase 2, mitochondrial. Antigen coverage aa 31-477 of 477

    Depositor

    Susanne Gräslund

    Article

    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Promoter

    LacZ

    Available Since

    April 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRG/III-hs-MBP-A2aTr316-H10

    Plasmid

    #121683

    Purpose

    Bacterial expression of adenosine A2a receptor fusion protein

    Depositor

    Richard Henderson, Reinhard Grisshammer

    Article

    Weiss et al Eur J Biochem. 2002 Jan;269(1):82-92.

    Insert

    adenosine A2a receptor (ADORA2A Human)
    

    Tags

    His10

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTYB3-human PUM1-HD MUT6-2 7-2 (N1043S-Q1047E-S1079N-E1083Q)-intein-CBD

    Plasmid

    #73300

    Purpose

    Expresses mutant human PUM1-HD

    Depositor

    Traci Hall

    Article

    Cheong et al Proc Natl Acad Sci U S A. 2006 Sep 12;10

    Insert

    human PUM1 Pumilio homology domain (PUM1 Human)
    

    Tags

    intein

    Expression

    Bacterial

    Mutation

    N1043S-Q1047E-S1079N-E1083Q

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

20. B2-2

    Plasmid

    #111469

    Purpose

    Thr-tRNA synthetase

    Depositor

    Cheemeng Tan

    Article

    Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

    Insert

    Thr-tRNA synthetase
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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