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Plasmid#109855PurposeProtein expression and purification of AP2M1_Adap-comp-subDepositorInsertAP2M1_Adap-comp-sub (AP2M1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
LHPP
Plasmid#39043PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertLHPP (LHPP Human)
UseTagsHis TevExpressionBacterialMutationSurface mutant for crystallization: Q58K59 -> …PromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKS012
Plasmid#65465PurposepSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) Ntag and mCherryDepositorInsertNtag-mCherry
UseSynthetic BiologyTagsFLAGExpressionBacterialMutationPromoterpl-TetOAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSC300
Plasmid#31820DepositorInsertGFP (lacking ATG start codon)
UseTagsGFP (lacking ATG start codon)ExpressionBacterialMutationPromoterSODAvailable sinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET_RGG-RGG_withTEVsite
Plasmid#124940PurposeExpression of RGG-RGGDepositorInsertRGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
UseTagsHisExpressionBacterialMutationwith TEV site between RGG domainsPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSETB-roGFP1-R10
Plasmid#83310PurposeInducible expression of redox sensitive GFP in bacteriaDepositorInsertroGFP1-R10
UseTags6xHISExpressionBacterialMutationC48S/Q80R/S147C/Q204C/F223R/S202K/K41DPromoterT7Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAP758-1
Plasmid#99494PurposeTEV::eGFP::3XFlagDepositorInsertTEV::eGFP::3XFlag
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCON1-ProB.gfp
Plasmid#112516PurposeProB driven gfpmut3 expression with synTTDepositorInsertProB gfpmut3 TT
UseTagsExpressionBacterialMutationPromoterProBAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
S13_EcoRV_UBC_AscI
Plasmid#128477PurposeUBC promoterDepositorInsertUBC promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
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PacrAB-rfp + PmutS-yfp
Plasmid#121442Purposeplasmid reporting the expression from the acrAB and mutS promotersDepositorInsertsPmutS-yfp
PacrAB-rfp
UseSynthetic Biology; Pbb vectorTagsExpressionBacterialMutationPromoterPacrAB and PmutSAvailable sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSUM-Kan-MCS1-gfp
Plasmid#109288PurposeExpresses high levels of gfp in mycobacteriaDepositorInsertMultiple Cloning Site and GFP
UseTagsExpressionBacterialMutationPromoterKanamycinAvailable sinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
TrpLE-M-nMSP1E3D1
Plasmid#179949PurposeTagless membrane scaffold protein (MSP) for formation of nanodiscs.DepositorInsertTrpLE-M-nMSP1E3D1
UseTagsTrpLEExpressionBacterialMutationNative Trp residues have been mutated to Phe resi…PromoterT7Available sinceFeb. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
ARFGAP1
Plasmid#25252PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertARFGAP1:M1-S128 (ARFGAP1 Human)
UseTagsHisExpressionBacterialMutationContains amino acids M1-S128PromoterAvailable sinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJMP1346
Plasmid#119274Purposeknockdown folA in K. pneumoniaeDepositorInsertsgRNA folA (K. pneumoniae)
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable sinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1341
Plasmid#119272Purposecontrol sgRNA in cells lacking rfpDepositorInsertsgRNA NT1/RR1 (rfp)
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS314-hUD
Plasmid#11005DepositorInserthUD recombination system
UseTagsExpressionBacterial and YeastMutationDirect repeat recombination system. Recombination…PromoterAvailable sinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
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G-SP140-8
Plasmid#166520PurposescFv of a human scaffold targeting Nuclear body protein SP140. Antigen coverage aa 688-862 of 867, BromodomainDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialMutationPromoterLacZAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSR:MSMEG_0319:Dendra
Plasmid#159010PurposeT7 expression of C-term fluorescent fusion in E. coliDepositorInsertMSMEG_0319
UseIntegrating mycobacterial expression vector attl5TagsGly-Ala Linker/Dendra2/1x Flag TagExpressionBacterialMutationPromoterpmycTetOAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only