-
Plasmid#166930PurposeStop codon part (E)DepositorInsertSTOP CODON TAA and spacer - BLOCK E- Flanked by BsaI sites
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pD-01
Plasmid#166929PurposeTrrnB and L3S2P21 terminator part (D)DepositorInsertDOUBLE TERMINATOR ( TrrnB - L3S2P21) _BLOCK D - Flanked by BsaI sites
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC-01
Plasmid#166925PurposesRNA part (C)DepositorInsertsRNA ToeHold - Block C- Flanked by BsaI sites
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB-01
Plasmid#166924PurposePtac promoter part (B)DepositorInsertPTAC promoter - Block B- Flanked by BsaI restriction sites.
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pA-01
Plasmid#166923PurposeLacI expression cassette (A)DepositorInsertLacI expression cassette - Block A- Flanked by BsaI sites
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSopBWT
Plasmid#112512PurposePsopB driven sopB and sigEDepositorInsertsopB/sigE operon
UseTagsExpressionBacterialMutationPromoterPsopBAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOGG037
Plasmid#113995PurposeSC module. Super-folder GFP green fluorescence reporterDepositorInsertSuper-folder GFP
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-GP-2-CBX1 Chromo
Plasmid#59697PurposeContains histone modification interacting domain (CBX1 Chromo)DepositorInsertCBX1 Chromo (CBX1 Human)
UseTagsGSTExpressionBacterialMutationPromotertac promoterAvailable sinceOct. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB-PAiRFP1
Plasmid#44270DepositorInsertPAiRFP1
UseTagsExpressionBacterialMutationG127D/S141R/M163L/Q168L/A203V/G218S/R220P/V244F/A…PromoterpBADAvailable sinceMay 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMEG_BB3_YL001
Plasmid#117825PurposeOverexpession plasmid of glycerol kinase (GUT1) in Y. lipolyticaDepositorInsertYlpTEF_YlGUT1_YlCyc1TT
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-INTS3-N
Plasmid#128416PurposeExpress INTS3 N-termini (1-513 aa) in E. coli with a GST tag (N-terminal)DepositorInsertINTS3 (INTS3 Human)
UseTagsGSTExpressionBacterialMutationN-terminal region (1–513 aa)Promotertac promoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-INTS3-C
Plasmid#128417PurposeExpress INTS3 C-termini (514-1042 aa) in E. coli with a GST tag (N-terminal)DepositorInsertINTS3 (INTS3 Human)
UseTagsGSTExpressionBacterialMutationINTS3 C-terminal region (514-1042 aa)PromoterTac promoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
pET28b(+)_S. cerevisiae Hbs1(1-611)
Plasmid#39336DepositorInsertElongation factor 1 alpha-like protein
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
MAGEA3
Plasmid#73246PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMAGEA3 (MAGEA3 Human)
UseTagsHis6-Thioredoxin-TEVExpressionBacterialMutationpfam01454:MAGE 116-286PromoterT7Available sinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCK300
Plasmid#87766Purposeempty control plasmid, three terminators to place inserts between for insulation, ampR, pBR322 origin, lacIDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterNoneAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSZBAD1PG
Plasmid#11964DepositorInsertSspDnaEC
UseTagsGB1ExpressionBacterialMutationPromoterAvailable sinceSept. 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET-HJURP-CBP
Plasmid#30393DepositorInsertHJURP CenpA Binding Domain (CENPA Budding Yeast, Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
msEGFP-PACR_pRSETB
Plasmid#55775PurposeGenetically encoded caged Ca2+DepositorInsertPACR
UseTags6xHis and msEGFPExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 3, 2014AvailabilityAcademic Institutions and Nonprofits only -