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Addgene
Showing: 11721 - 11740 of 27133 results

  1. pJK614

    Plasmid
    #72387
    Purpose
    Produces Escherichia coli glycinamide ribonucleotide synthetase (EcPurDH6)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylamine-glycine ligase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    contains 4 silent mutations in codons 9-11 and 42…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72387-plasmid-map-sequence-id-133927

    Enlarge

  2. pJK621

    Plasmid
    #72388
    Purpose
    Produces Escherichia coli glycinamide ribonucleotide synthetase (EcPurDH6)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylamine-glycine ligase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    contains 3 silent mutations in codons 9-11 to fac…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72388-plasmid-map-sequence-id-133928

    Enlarge

  3. pJK630

    Plasmid
    #72389
    Purpose
    Produces Acetobacter aceti 1023 phosphoribosylpyrophosphate synthetase (AaPrsA-insV2)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylpyrophosphate synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    inserted Val2
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72389-plasmid-map-sequence-id-133978

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  4. pJK631

    Plasmid
    #72390
    Purpose
    Produces Acetobacter aceti 1023 phosphoribosylpyrophosphate synthetase (AaPrsAH6-insA2), C-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylpyrophosphate synthetase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    inserted Ala2 to facilitate cloning
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72390-plasmid-map-sequence-id-133987

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  5. pJK642

    Plasmid
    #72391
    Purpose
    Produces Escherichia coli glutamine synthetase, N-terminal His6 tag and Y397F mutation that prevents adenylation (H6EcGlnA-Y397F)
    Depositor
    T. J. Kappock
    Insert
    glutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutated tyrosine-397 to phenylalanine to block ad…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72391-plasmid-map-sequence-id-133988

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  6. pJK633

    Plasmid
    #72397
    Purpose
    Produces Escherichia coli aminoimidazole ribonucleotide synthetase, N-terminal His6 tag (H6EcPurM)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole synthetase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72397-plasmid-map-sequence-id-134629

    Enlarge

  7. pJK639

    Plasmid
    #72398
    Purpose
    Produces Escherichia coli phosphoribosylpyrophosphate amidotransferase, C-terminal His6 tag (EcPurFH6)
    Depositor
    T. J. Kappock
    Insert
    amidophosphoribosyltransferase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72398-plasmid-map-sequence-id-133991

    Enlarge

  8. pJK649

    Plasmid
    #72399
    Purpose
    Produces Escherichia coli phosphoribosylpyrophosphate amidotransferase (EcPurF)
    Depositor
    T. J. Kappock
    Insert
    amidophosphoribosyltransferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72399-plasmid-map-sequence-id-133993

    Enlarge

  9. pJK650

    Plasmid
    #72400
    Purpose
    Produces Escherichia coli glutamine synthetase with Y397F mutation that prevents adenylation (EcGlnA-Y397F)
    Depositor
    T. J. Kappock
    Insert
    glutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutated tyrosine-397 to phenylalanine to block ad…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72400-plasmid-map-sequence-id-133994

    Enlarge

  10. pJK361

    Plasmid
    #72403
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase, N-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    succinyl-CoA:acetate CoA-transferase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72403-plasmid-map-sequence-id-134630

    Enlarge

  11. pJK365

    Plasmid
    #72404
    Purpose
    Produces Acetobacter aceti 1023 Est01
    Depositor
    T. J. Kappock
    Insert
    acyl-CoA thioester hydrolase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72404-plasmid-map-sequence-id-134631

    Enlarge

  12. pJK366

    Plasmid
    #72405
    Purpose
    Produces Acetobacter aceti 1023 Est02
    Depositor
    T. J. Kappock
    Insert
    peptidase S9
    Use
    Tags
    Expression
    Bacterial
    Mutation
    additional N-terminal MVKNGS sequence, alternate …
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72405-plasmid-map-sequence-id-135520

    Enlarge

  13. pJK367

    Plasmid
    #72406
    Purpose
    Produces Acetobacter aceti 1023 Est06
    Depositor
    T. J. Kappock
    Insert
    lipase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    lacks Met1 and Thr2 in annotated (v1) sequence
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72406-plasmid-map-sequence-id-134674

    Enlarge

  14. pJK368

    Plasmid
    #72407
    Purpose
    Produces Acetobacter aceti 1023 Est12
    Depositor
    T. J. Kappock
    Insert
    acyl-CoA thioesterase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    additional N-terminal 15 residues MSETYGTGQKEQHC,…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72407-plasmid-map-sequence-id-135653

    Enlarge

  15. pJK373

    Plasmid
    #72408
    Purpose
    Produces Acetobacter aceti 1023 Est03
    Depositor
    T. J. Kappock
    Insert
    4-hydroxybenzoyl-CoA thioesterase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72408-plasmid-map-sequence-id-135523

    Enlarge

  16. pJK374

    Plasmid
    #72409
    Purpose
    Produces Acetobacter aceti 1023 Est10
    Depositor
    T. J. Kappock
    Insert
    feruloyl esterase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72409-plasmid-map-sequence-id-135540

    Enlarge

  17. pJK375

    Plasmid
    #72410
    Purpose
    Produces Acetobacter aceti 1023 Est13
    Depositor
    T. J. Kappock
    Insert
    arylesterase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72410-plasmid-map-sequence-id-135659

    Enlarge

  18. pJK377

    Plasmid
    #72411
    Purpose
    Produces Acetobacter aceti 1023 Est05
    Depositor
    T. J. Kappock
    Insert
    hypothetical protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    additional N-terminal MTFLYSICHIVMNIVSSTPHFNVWICF…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72411-plasmid-map-sequence-id-135530

    Enlarge

  19. pJK378

    Plasmid
    #72412
    Purpose
    Produces Acetobacter aceti 1023 Est07
    Depositor
    T. J. Kappock
    Insert
    4-hydroxybenzoyl-CoA thioesterase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72412-plasmid-map-sequence-id-135534

    Enlarge

  20. pJK379

    Plasmid
    #72413
    Purpose
    Produces Acetobacter aceti 1023 Est14
    Depositor
    T. J. Kappock
    Insert
    arylesterase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72413-plasmid-map-sequence-id-135785

    Enlarge

Showing: 11721 - 11740 of 27133 results