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Plasmid#48118PurposeShuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein YngK under control of the xylose inducible promoterDepositorInsertsignal peptide of YngK
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSLAX13 HA (CT#233)
Plasmid#14027DepositorTypeEmpty backboneUseTags3x HAExpressionBacterialMutationPromoterAvailable sinceFeb. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
pZSm45 sodA
Plasmid#48890PurposeLux-regulated expression of sodA enzymeDepositorInsertsodA LuxR
UseSynthetic BiologyTagsssrA tag (AANDENYALAA)ExpressionBacterialMutationPromoterpLuxAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS415-SPT6
Plasmid#10993DepositorInsertSPT6 (SPT6 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationWild typePromoterAvailable sinceDec. 29, 2005AvailabilityAcademic Institutions and Nonprofits only -
pOGG014
Plasmid#113988PurposeVector construction module. Endlinker/terminator module to circularize plasmid following Part 4DepositorInsertEndlinker/terminator Part 4
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
39 pCOLI_K_SUMO
Plasmid#160182PurposeN-term 6xHis SUMO 3C cleavable Kan resistant bacterial expression vectorDepositorTypeEmpty backboneUseTags6xHis and SUMOExpressionBacterialMutationPromoterT7Available sinceNov. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZH051
Plasmid#47311PurposeExpresses Tsr-Venus-Ubiquitin-CIDepositorInsertwild type immunity region of lambda phage with tsr-venus-ub-cI
UseTagsCIExpressionBacterialMutationPromoterPR; PRMAvailable sinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-SunTag
Plasmid#198300PurposeProduces PVCs retargeted with SunTagDepositorInsertPVCpnf_pvc13-SunTag
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
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FKBP-YPet-RanGAP*
Plasmid#175245PurposeBacterial expression and purification, SUMOylation substrate that can be recruited to FRB with rapamycin, Ypet is a FRET acceptor for CyPetDepositorInsertRANGAP1 (RANGAP1 Human)
UseTagsExpressionBacterialMutationAmino acid 562 F to APromotertacAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
CyPet-FKBP-E2
Plasmid#175244PurposeBacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycinDepositorInsertUBE2I (UBE2I Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
PP-HSP90 (3Q5J)
Plasmid#28281DepositorInsertPP-HSP90
UseTagsHis tag with TEV cleavageExpressionBacterialMutationN-terminal domainPromoterAvailable sinceAug. 8, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHH0103_RARB_Hormone-recep
Plasmid#109845PurposeProtein expression and purification of RARB_Hormone-recepDepositorInsertRARB_Hormone-recep (RARB Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGT-B3
Plasmid#122581PurposeMAGIC donor plasmid (pBBR1 oriR with beta-lactamase/sfGFP payload)DepositorInsertsfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046832
Plasmid#145691PurposeBacterial Expression plasmid for SARS-CoV-2 nsp7DepositorInsertSARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;StrepIIExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pReporter_11
Plasmid#60571PurposeA reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).DepositorInsertReporter_11
UseTagsExpressionBacterialMutationPromoterBBa_J23119Available sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pAP1698-4
Plasmid#105242Purposemammalian eGFPDepositorInserteGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
SW5
Plasmid#40108DepositorInsertBistable switch using dynamic degradation
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 12, 2012AvailabilityAcademic Institutions and Nonprofits only