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pRAM18dSGK[MCS]
Plasmid#84684PurposeCan be used for the transformation of rickettsiaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmSod1-G93A-N’His
Plasmid#117705Purposemutation on stability/solubility of SOD1DepositorInsertSod1 (Sod1 Mouse)
UseTagsHis tag with Thrombin cleavage siteExpressionBacterialMutationG93APromoterT7Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+) 6xHis-TEV-IFIT5
Plasmid#53560Purposebacterial expression of IFIT5DepositorInsertIFIT5 (IFIT5 Human)
UseTags6xHIS and TEVExpressionBacterialMutationPromoterT7Available sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBad-CA TAG93
Plasmid#105667Purposeexpresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 93 and C-terminal 6x his tagDepositorArticleInserthuman carbonic anhydrase II thermostable variant (CA2 Human)
UseTags6x his tagExpressionBacterialMutationTAG stop codon at site 93PromoterpBad/arabinoseAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
RBFOX3_RRM_pGEX
Plasmid#135131PurposeRecombinant expression of RBFOX3 with dual-affinity tagDepositorInsertRBFOX3 (RBFOX3 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceMarch 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pE-SUMO CRYGS
Plasmid#80753PurposeExpression of human gamma S-crystallin in E. coliDepositorInsertCRYGS (CRYGS Human)
UseTags6xHIS and Smt3 SUMOExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Orf10
Plasmid#156486PurposeBacterial expression of Sars-CoV2 Orf10 protein with His-tag and GST-tagDepositorInsertOrf10 (ORF10 Severe acute respiratory syndrome coronavirus 2, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMIND-Lx
Plasmid#25013DepositorInsertpMIND-Lx
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBS mTSP-5
Plasmid#12996DepositorInsertTSP-5 (Comp Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCFP
Plasmid#28011DepositorInsertCFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(RBSmut)_Psyn-sgRNAflaB
Plasmid#149588Purposeall-in-one CRISPRi vector for targeting B. burgdorferi flaBDepositorInsertdCas9, lacI, sgRNAflaB
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PsynAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSHS248
Plasmid#87354PurposeS867C/N1054C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-2 FRET constructDepositorInsertCas9
UseTagsHis-MBPExpressionBacterialMutationC80S/C574S/S867C/N1054CPromoterT7Available sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNVLTv2
Plasmid#169245PurposeGeneric suicide vector for knockouts and integrationsDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET30a-SRAP-delCys2
Plasmid#84568PurposeEncodes murine SRAP domain (250aa) of Hmces/Srap1 with deletion of catalytic Cys2DepositorInsertSrap1 (Hmces Mouse)
UseTags6xHis and S-TagExpressionBacterialMutationdeletion of Cys2PromoterT7Available sinceNov. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS huNFATc4-EE
Plasmid#17869DepositorInsertNFATc4 (NFATC4 Human)
UseTagsEEExpressionBacterialMutationPromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pECFP-Drp1
Plasmid#191942PurposeExpression of a Drp1-ECFP chimeraDepositorInsertDrp1
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMTS-HA-GFP11
Plasmid#177725PurposeEleventh β-strand of GFP (GFP11) fused to a mitochondria signal sequence for mitochondrial targeting, HA taggedDepositorInsertMTS-HA-GFP11
UseTagsGFP11 and HAExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
PHKG2_HUMAN_D0
Plasmid#79736PurposeThis plasmid encodes the kinase domain of PHKG2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertPHKG2 (PHKG2 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
VRK2_HUMAN_D0
Plasmid#79737PurposeThis plasmid encodes the kinase domain of VRK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertVRK2 (VRK2 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only
